MULTIPLEX EDITING WITH CAS ENZYMES

1 - 73 . (canceled)

74 . A method of editing two or more loci within a cell, said method comprising introducing into said cell:

a) a class 2, type II Cas endonuclease or a nucleic acid encoding said class 2, type II Cas endonuclease; and

b) a first engineered guide ribonucleic acid or a nucleic acid encoding said first engineered guide ribonucleic acid, wherein said first engineered guide ribonucleic acid comprises:

i) a first ribonucleic acid sequence configured to bind to said class 2, type II Cas endonuclease, and

ii) a first spacer sequence configured to hybridize to a first set of one or more target loci; and

c) a class 2, type V Cas endonuclease or a nucleic acid encoding said class 2, type V Cas endonuclease; and

d) a second engineered guide ribonucleic acid or a nucleic acid encoding said second engineered guide ribonucleic acid, wherein said second engineered guide ribonucleic acid comprises:

i) a second ribonucleic acid sequence configured to bind to said class 2, type V Cas endonuclease, and

ii) a second spacer sequence configured to hybridize to a second set of one or more target loci.

75 . The method of claim 74 , wherein said class 2, type II Cas endonuclease comprises a sequence having at least 75% sequence identity to SEQ ID NO: 1.

76 . The method of claim 74 , wherein said class 2, type V Cas endonuclease is a Cas12a endonuclease.

77 . The method of claim 74 , wherein said class 2, type V Cas endonuclease comprises a sequence having at least 75% sequence identity to SEQ ID NO: 7.

78 . The method of claim 74 , wherein said class 2, type II Cas endonuclease comprises a sequence having at least 85% sequence identity to SEQ ID NO: 1, and said class 2, type V Cas endonuclease comprises a sequence having at least 85% sequence identity to SEQ ID NO: 7.

79 . The method of claim 78 , wherein said class 2, type II Cas endonuclease comprises a sequence of SEQ ID NO: 1, and said class 2, type V Cas endonuclease comprises a sequence of SEQ ID NO: 7.

80 . The method of claim 79 , wherein said first engineered guide ribonucleic acid comprises a sequence of SEQ ID NO: 3 and said second engineered guide ribonucleic acid comprises a sequence of SEQ ID NO: 9.

81 . The method of claim 74 , wherein said first engineered guide ribonucleic acid comprises a sequence having at least 80% sequence identity to SEQ ID NO: 3.

82 . The method of claim 74 , wherein said second engineered guide ribonucleic acid comprises a sequence having at least 80% sequence identity to SEQ ID NO: 9.

83 . The method of claim 74 , wherein said first set of one or more target loci or said second set of one or more target loci comprises a T-cell receptor (TCR) locus, an albumin (ALB) locus or a Nuclear Receptor Subfamily 3 Group C Member 1 (NR3C1) locus.

84 . The method of claim 74 , wherein said first spacer sequence configured to hybridize to said first set of one or more target loci or said second spacer sequence configured to hybridize to said second set of one or more target loci has at least 80% sequence identity to any one of SEQ ID NOs: 10-22 or a complement thereof.

85 . The method of claim 74 , wherein said editing comprises insertion of an indel, a premature termination codon, a missense codon, a frameshift mutation, an adenine deamination, a cytosine deamination, or any combination thereof.

86 . A method of making a glucocorticoid-resistant engineered T cell, said method comprising introducing to a T-cell or a precursor thereof:

a) a first ribonucleic acid guided endonuclease or a nucleic acid encoding said first ribonucleic acid guided endonuclease; and

b) a first engineered guide ribonucleic acid or a nucleic acid encoding said first engineered guide ribonucleic acid, said first engineered guide ribonucleic acid comprising:

i) a first ribonucleic acid sequence configured to form a complex with said first ribonucleic acid guided endonuclease, and

ii) a first spacer sequence configured to hybridize to at least part of a T-cell receptor (TCR) locus; and

c) a second ribonucleic acid guided endonuclease or a nucleic acid encoding said second ribonucleic acid guided endonuclease; and

d) a second engineered guide ribonucleic acid or a nucleic acid encoding said second engineered guide ribonucleic acid, said second engineered guide ribonucleic acid comprising:

i) a second ribonucleic acid sequence configured to form a complex with said second ribonucleic acid guided endonuclease, and

ii) a second spacer sequence configured to hybridize to at least part of a T-cell receptor Nuclear Receptor Subfamily 3 Group C Member 1 (NR3C1) locus.

87 . The method of claim 86 , wherein said first ribonucleic acid guided endonuclease is a class 2, type II Cas endonuclease and said second ribonucleic acid guided endonuclease is a class 2, type V Cas endonuclease.

88 . A population of glucocorticoid-resistant T cells, comprising:

a) a heterologous sequence within 100, 75, 50, 25, or 10 nucleotides of a hybridization region of any one of SEQ ID NOs: 10-15 within a TCR locus; and

b) an NR3C1 locus comprising an indel.

89 . The population of glucocorticoid-resistant T cells of claim 88 , wherein said heterologous sequence is an indel.

90 . An isolated nucleic acid comprising any one of SEQ ID NOs: 63-65 or a sequence having at least 75% sequence identity thereto.

91 . A cell comprising said isolated nucleic acid of claim 90 .

92 . The cell of claim 91 , wherein said cell is a T-cell or precursor thereof.

93 . A vector comprising said isolated nucleic acid sequence of claim 90 .