Granted Patent B2

US 12,280,370 · App. 18/466,696 · Granted Apr 22, 2025

Inventors: Randall D. Lowe, Jr. (Emeryville, CA); Alexander J. Mastroianni (Alameda, CA); Mark P. White (Orinda, CA); Gregory G. Lavieu (Vitry sur Seine, FR); Kristin G. Beaumont (New York, NY)

Assignee: Bruker Cellular Analysis, Inc.

B01L3/502707B01L3/502715B01L3/502761B81C1/00206C12M23/16G01N33/54393B01L2300/0636B01L2300/0816B01L2300/0864B01L2300/088B81B2201/058

Abstract

Claims (24)

1. A kit including:

a microfluidic device comprising: an enclosure including a base, a cover, and microfluidic circuit material defining a fluidic circuit therein, where at least one inner surface of the base, the cover and the microfluidic circuit material has a plurality of first covalently bound surface modifications, each including a first linking group and a first moiety, where the first moiety is a first reactive moiety or a first surface contact moiety;

where at least one inner surface of the base, the cover and the microfluidic circuit material has a plurality of second covalently bound surface modifications, each including a second linking group and a second moiety, where the second moiety is a second reactive moiety or a second surface contact moiety; and

a first surface modifying reagent having a structure of Formula XII 1 :

RP 1 -L 1 -surface contact moiety 1   (Formula XII 1 )

wherein RP 1 is a reaction pair moiety; surface contact moiety 1 is a moiety configured to support cell growth, viability, portability, or any combination thereof; and L 1 is a linker; wherein L 1 is a bond or a chain of 1 to 200 non-hydrogen atoms selected from any combination of silicon, carbon, nitrogen, oxygen, sulfur and phosphorus atoms, and may further include 0 or 1 coupling groups CG.

2. The kit of claim 1 , wherein the first moiety is a first reactive moiety.

3. The kit of claim 2 , wherein the first reactive moiety comprises an alkyne moiety, an azide moiety, a carboxylic acid moiety, an amine moiety, an olefinic moiety, a tetrazinyl moiety, a trans-cyclooctenyl moiety, a thiol moiety, a maleimide moiety, a biotin moiety, a streptavidin moiety, a halide moiety, a cyano moiety, isocyanate moiety, an epoxide moiety, a hydroxyamine moiety, or a sulfonyl fluoride moiety; and/or where the second reactive moiety comprises an alkyne moiety, an azide moiety, a carboxylic acid moiety, an amine moiety, an olefinic moiety, a tetrazinyl moiety, a trans-cyclooctenyl moiety, a thiol moiety, a maleimide moiety, a biotin moiety, a streptavidin moiety, a halide moiety, a cyano moiety, isocyanate moiety, an epoxide moiety, a hydroxyamine moiety, or a sulfonyl fluoride moiety.

4. The kit of claim 2 , wherein the first reactive moiety has a structure selected from Formula XXX, Formula V, and Formula VII:

where: LG is —W—Si(OZ) 2 O— or —OP(O) 2 O—; L fm is a linker including 1 to 200 non-hydrogen atoms selected from any combination of silicon, carbon, nitrogen, oxygen, sulfur and phosphorus atoms and may further include 0 or 1 coupling groups CG; R x is a reactive moiety; W is O, S, or N; Z is a bond to an adjacent silicon atom or is a bond to the surface; n is an integer of 3 to 21; and

is the surface.

5. The kit of claim 2 , wherein the second moiety is a second reactive moiety.

6. The kit of claim 5 , wherein the second reactive moiety comprises an alkyne moiety, an azide moiety, a carboxylic acid moiety, an amine moiety, an olefinic moiety, a tetrazinyl moiety, a trans-cyclooctenyl moiety, a thiol moiety, a maleimide moiety, a biotin moiety, a streptavidin moiety, a halide moiety, a cyano moiety, isocyanate moiety, an epoxide moiety, a hydroxyamine moiety, or a sulfonyl fluoride moiety; and/or where the second reactive moiety comprises an alkyne moiety, an azide moiety, a carboxylic acid moiety, an amine moiety, an olefinic moiety, a tetrazinyl moiety, a trans-cyclooctenyl moiety, a thiol moiety, a maleimide moiety, a biotin moiety, a streptavidin moiety, a halide moiety, a cyano moiety, isocyanate moiety, an epoxide moiety, a hydroxyamine moiety, or a sulfonyl fluoride moiety.

7. The kit of claim 5 , wherein the second reactive moiety has a structure selected from Formula XXX′, Formula V′, and Formula VII′:

where: LG′ is —W—Si(OZ) 2 O— or —OP(O) 2 O—; L′ fm is a linker including 1 to 200 non-hydrogen atoms selected from any combination of silicon, carbon, nitrogen, oxygen, sulfur and phosphorus atoms and may further include 0 or 1 coupling groups CG; R′ x is a reactive moiety; W′ is O, S, or N; Z′ is a bond to an adjacent silicon atom or is a bond to the surface; n′ is an integer of 3 to 21; and is the surface.

8. The kit of claim 5 , wherein the first reactive moiety and the second reactive moiety have the same formula.

9. The kit of claim 1 , wherein the surface contact moiety 1 is polymeric.

10. The kit of claim 1 , wherein the surface contact moiety includes one or more of an alkyl, fluoroalkyl, monosaccharide, polysaccharide, alcohol, polyalcohol, alkylene ether, polyelectrolytes, amino, carboxylic acid, phosphonic acid, sulfonate anion, carboxybetaines, sulfobetaine, sulfamic acid, amino acid moiety, or cleavable moiety.

11. The kit of claim 1 further comprising a second surface modifying reagent having a structure of Formula XII 2 :

RP 2 -L 2 -surface contact moiety 2   (Formula XII 2 )

wherein RP 2 is a reaction pair moiety; surface contact moiety 2 is a moiety configured to support cell growth, viability, portability, or any combination thereof; L 2 is a linker; wherein L 2 is a bond or 1 to 200 non-hydrogen atoms selected from any combination of silicon, carbon, nitrogen, oxygen, sulfur and phosphorus atoms, and may further include 0 or 1 coupling groups CG; and

wherein the second surface modifying reagent is different than the first surface modifying reagent.

12. The kit of claim 11 , wherein the surface contact moiety 2 is polymeric.

13. The kit of claim 11 , wherein the surface contact moiety 2 includes one or more of an alkyl, fluoroalkyl, monosaccharide, polysaccharide, alcohol, polyalcohol, alkylene ether, polyelectrolytes, amino, carboxylic acid, phosphonic acid, sulfonate anion, carboxybetaines, sulfobetaine, sulfamic acid, amino acid moiety, or cleavable moiety.

Assignments (3)

Continuity (9)

References Cited (367)