ANTI-TRANSFERRIN RECEPTOR ANTIBODIES AND USES THEREOF
Disclosed herein, in certain embodiments, are anti-transferrin receptor antibodies, anti-transferrin receptor antibody conjugates, and pharmaceutical compositions which comprise the anti-transferrin receptor antibodies or conjugates. In some embodiments, also disclosed herein are methods of delivering a payload utilizing an anti-transferrin receptor antibody described herein, and methods of treatment with use of an anti-transferrin receptor antibody described herein.
1 . A method of treating muscular dystrophy in a subject in need thereof, comprising:
administering to the subject an anti-transferrin receptor antibody conjugate comprising i) anti-transferrin receptor antibody comprising a variable heavy chain (VH) region and a variable light chain (VL) region, wherein the VH region comprises HCDR1 sequence comprising SEQ ID NO: 1; HCDR2 sequence EINPIX 1 GRSNYAX 2 KFQG (SEQ ID NO: 88), wherein X 1 is selected from N or Q and X 2 is selected from Q or E; and HCDR3 sequence comprising SEQ ID NO: 3, wherein the VL region comprises LCDR1 sequence RTSENIYX 3 NLA (SEQ ID NO: 89), LCDR2 sequence AX 4 TNLAX 5 (SEQ ID NO: 90), and LCDR3 sequence QHFWGTPLTX 6 (SEQ ID NO: 91), wherein X 3 is selected from N or S, X 4 is selected from A or G, X 5 is selected from D or E, and X 6 is present or absence, and if present, is F, and ii) a payload, wherein the payload hybridizes to a target region of an mRNA that causes muscular dystrophy, thereby treating muscular dystrophy in the subject.
2 . The method of claim 1 , wherein the payload comprises a small molecule, a peptide, a protein, or a polynucleic acid molecule.
3 . The method of claim 2 , wherein the polynucleic acid molecule is a single stranded polynucleotide.
4 . The method of claim 3 , wherein the single stranded polynucleotide is a phosphorodiamidate morpholino oligonucleotide (PMO).
5 . The method of claim 1 , wherein the muscular dystrophy is Duchenne Muscular Dystrophy (DMD), Becker muscular dystrophy, facioscapulohumeral muscular dystrophy (FSHD), or congenital muscular dystrophy.
6 . The method of claim 1 , wherein the muscular dystrophy is Duchenne Muscular Dystrophy (DMD).
7 . The method of claim 1 , wherein reticulocyte levels in the subject are not reduced following the administration of the anti-transferrin receptor antibody.
8 . The method of claim 1 , wherein the administration of the anti-transferrin receptor antibody conjugate delivers the payload to the target region of the mRNA.
9 . The method of claim 6 , wherein the payload is a polynucleic acid molecule that hybridizes to a target region of an mRNA of the DMD gene.
10 . The method of claim 9 , wherein the target region of the mRNA has an incorrectly spliced mRNA transcript.
11 . The method of claim 10 , wherein the polynucleic acid molecule induces exon skipping or exon inclusion of the incorrectly spliced mRNA transcript in the subject.
12 . The method of claim 11 , wherein the exon skipping or exon inclusion is in muscle.
13 . The method of claim 12 , wherein the muscle is skeletal muscle.
14 . The method of claim 12 , wherein the muscle is cardiac muscle.
15 . The method of claim 9 , wherein the target region of the mRNA has at least one mutated exon in the DMD gene.
16 . The method of claim 15 , wherein the at least one mutated exon of the DMD gene is an exon selected from the group consisting of exon 3, 4, 5, 6, 7, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, and 63.
17 . The method of claim 15 , wherein the at least one mutated exon of the DMD gene is an exon selected from the group consisting of exon 8, 23, 35, 43, 44, 45, 50, 51, 52, 53, and 55.
18 . The method of claim 15 , wherein the at least one mutated exon of the DMD gene is an exon selected from the group consisting of exon 44, 45, 50, 51, and 52.
19 . The method of claim 1 , wherein the anti-transferrin receptor antibody comprises a humanized antibody or antigen binding fragment thereof, a chimeric antibody or antigen binding fragment thereof, or a multi-specific antibody or antigen binding fragment thereof.
20 . The method of claim 1 , wherein the anti-transferrin receptor antibody comprises an IgG-scFv, nanobody, BiTE, diabody, DART, TandAb, scDiabody, scDiabody-CH3, triple body, mini-antibody, minibody, TriBi minibody, scFv-CH3 KIH, Fab-scFv-Fc KIH, Fab-scFv, scFv-CH-CL-scFv, Fab′, F(ab′)2, F(ab′)3, F(ab′)2-scFv2, scFv, scFv-KIH, Fab-scFv-Fc, tetravalent HCAb, scDiabody-Fc, diabody-Fc, tandem scFv-Fc, or intrabody.
21 . The method of claim 1 , wherein the anti-transferrin receptor antibody further comprises at least one mutation in the Fc region, and the at least one mutation is at residue position D265, N297, K322, L328, or P329, wherein the residue position is in reference to IgG1.
22 . The method of claim 1 , wherein the anti-transferrin receptor antibody further comprises at least one mutation in the Fc region at L233 and L234, and wherein the residues correspond to position 233 and 234 of SEQ ID NO: 23.