PROCESS FOR OBTAINING A PURIFIED PSYCHOACTIVE ALKALOID SOLUTION

1 . A process for obtaining a purified psychoactive alkaloid solution, the process comprising:

contacting a psychoactive alkaloid extract in liquid or slurry form with an adsorbent material to obtain adsorbed psychoactive alkaloid, wherein:

the psychoactive alkaloid extract in liquid or slurry form comprises a primary aliphatic alcohol, a ketone, water or any combination selected therefrom and has a pH between 2.5-4.5 or 9-10;

the psychoactive alkaloid extract comprises psychoactive alkaloid that is psilocybin, psilocin, baeocystin, norbaeocystin, norpsilocin, aeruginascin, bufotenin, bufotenidine, 5-Me0-DMT (5-methoxy-N,N-dimethyltryptamine), N,N-dimethyltryptamine (DMT), ergine (LSA), ergonovine, ergometrine, muscimol, ibotenic acid, lysergic acid hydroxyethylamide (LSH), elymoclavine, ergometrinine, chanoclavine or any combination selected therefrom; and

the adsorbent material is a gel resin, a first non-ionic macroporous resin, an ion-exchange macroporous resin or any combination selected therefrom;

washing the adsorbent material with a first solvent that is water, a C1-4 primary aliphatic alcohol, a C3-4 ketone or any combination selected therefrom to remove one or more impurities from the adsorbent material; and

eluting the adsorbed psychoactive alkaloid from the adsorbent material using a second solvent different to the first solvent to obtain the purified psychoactive alkaloid solution, wherein the second solvent is water, a C1-4 primary aliphatic alcohol, a C3-4 ketone or any combination selected therefrom, under basic, acidic or neutral pH.

2 . The process of claim 1 , wherein the pH between 2.5-4.5 of the psychoactive alkaloid extract in liquid or slurry form is obtained by adding an acid to the psychoactive alkaloid extract, wherein the acid is one or more acids selected from the group consisting of acetic acid, adipic acid, ascorbic acid, phosphoric acid, ammonium aluminum sulphate, ammonium citrate dibasic, ammonium citrate monobasic, calcium citrate, calcium fumarate, calcium gluconate, calcium phosphate dibasic, calcium phosphate, hydrochloric acid, sulphuric acid monobasic, calcium phosphate tribasic, citric acid, fumaric acid, gluconic acid, magnesium fumarate, malic acid, phosphoric acid, potassium acid tartrate, potassium citrate, potassium fumarate, sodium citrate, sodium fumarate, sodium gluconate, sodium lactate, sodium potassium hexametaphosphate, sodium potassium tartrate, sodium potassium tripolyphosphate, sodium pyrophosphate tetrabasic, sodium tripolyphosphate and tartaric acid.

3 . The process of claim 1 , wherein the pH between 9-10 of the psychoactive alkaloid extract in liquid or slurry form is obtained by adding a base to the psychoactive alkaloid extract, wherein the base is one or more bases selected from the group consisting of ammonium bicarbonate, ammonium carbonate, ammonium hydroxide, calcium acetate, calcium carbonate, calcium chloride, calcium hydroxide, calcium lactate, calcium oxide, calcium phosphate dibasic, calcium phosphate monobasic, magnesium carbonate, potassium aluminum sulphate, potassium bicarbonate, potassium carbonate, potassium hydroxide, potassium lactate, potassium phosphate dibasic, potassium pyrophosphate tetrabasic, potassium phosphate tribasic, potassium tripolyphosphate, sodium acetate, sodium acid pyrophosphate, sodium aluminum phosphate, sodium aluminum sulphate, sodium bicarbonate, sodium bisulphate, sodium carbonate, sodium hexametaphosphate, sodium hydroxide, sodium lactate, sodium phosphate dibasic, sodium phosphate monobasic and sodium phosphate tribasic.

4 . The process of claim 1 , wherein the second solvent has a lower polarity than the first solvent.

5 . The process of claim 1 , wherein the process comprises further purifying the purified psychoactive alkaloid solution by repeating, with the purified psychoactive alkaloid solution, the contacting step with a different adsorbent material, the washing step with another solvent that is water, a C1-4 primary aliphatic alcohol, a C3-4 ketone or any combination selected therefrom, and the eluting step with yet another solvent that is water, a C1-4 primary aliphatic alcohol, a C3-4 ketone or any combination selected therefrom.

6 . The process of claim 1 , comprising:

evaporating a portion of the second solvent from the purified psychoactive alkaloid solution to obtain a purified psychoactive alkaloid slurry.

7 . The process of claim 6 , wherein the purified psychoactive alkaloid slurry comprises 5% by weight or more of the psychoactive alkaloid.

8 . The process of claim 6 , comprising:

standardizing the purified psychoactive alkaloid slurry by adding thereto a quantity of excipient measured to provide a specific concentration of the psychoactive alkaloid when the purified psychoactive alkaloid slurry is dried; and

drying the purified psychoactive alkaloid slurry by evaporating the remaining portion of the second solvent to obtain a standardized, purified, powdered psychoactive alkaloid extract having the specific concentration of the psychoactive alkaloid.

9 . The process of claim 8 , wherein the specific concentration is 0.1-99% by weight.

10 . The process of claim 8 , wherein the excipient is one or more excipients selected from the group consisting of silicon dioxide, ascorbic acid, maltodextrin, gum arabic, microcrystalline cellulose, sodium benzoate, sodium phosphate, sodium citrate, rice hulls and rice.

11 . The process of claim 1 , comprising:

prior to the contacting step:

adding an acid to the psychoactive alkaloid extract to bring its pH to 4±0.5; and

removing solids from the psychoactive alkaloid extract;

wherein:

the adsorbent material is the first non-ionic macroporous resin;

the first solvent is water; and

the second solvent is a hydro-ethanol solvent.

12 . The process of claim 11 , wherein the hydro-ethanol solvent is 5% ethanol.

13 . The process of claim 1 , comprising:

prior to the contacting step, adding an acid to the psychoactive alkaloid extract to bring its pH to 3±0.5;

after the contacting step and before the washing step, washing the adsorbent material with 100% ethanol, wherein the adsorbent material is a microporous strong cation exchange resin in an H+ or an Na+ form; and

after the eluting step:

adding alkali to the purified psychoactive alkaloid solution to bring its pH to 4±0.5;

removing solids from the purified psychoactive alkaloid solution;

evaporating a portion of the second solvent from the purified psychoactivealkaloid solution;

removing further solids from the purified psychoactive alkaloid solution;

contacting the purified psychoactive alkaloid solution with a second non-ionic macroporous resin to obtain second adsorbed psychoactive alkaloid;

washing the second non-ionic macroporous resin with purified water; and

eluting the second adsorbed psychoactive alkaloid from the second non-ionic macroporous resin using a hydro-ethanol solvent to obtain a twice purified psychoactive alkaloid solution;

wherein:

the first solvent is purified water; and

the second solvent is 2% hydrochloric acid and 80% ethanol.

14 . The process of claim 1 , comprising:

prior to the contacting step, adding a base to the psychoactive alkaloid extract to bring its pH to 9.5±0.5;

after the contacting step and before the washing step, washing the adsorbent material with 100% ethanol, wherein the adsorbent material is a microporous strong anion exchange resin in an OH— or a Cl— form; and

after the eluting step:

adding acid to the purified psychoactive alkaloid solution to bring its pH to 4±0.5;

removing solids from the purified psychoactive alkaloid solution;

evaporating a portion of the second solvent from the purified psychoactive alkaloid solution;

removing further solids from the purified psychoactive alkaloid solution;

contacting the purified psychoactive alkaloid solution with a second non-ionic macroporous resin to obtain second adsorbed psychoactive alkaloid;

washing the second non-ionic macroporous resin with purified water; and

eluting the second adsorbed psychoactive alkaloid from the second non-ionic macroporous resin using a hydro-ethanol solvent to obtain a twice purified psychoactive alkaloid solution;

wherein:

the first solvent is purified water; and

the second solvent is 2% sodium chloride and 80% ethanol.

15 . The process of claim 1 , wherein the psychoactive alkaloid extract is extracted from a psychoactive alkaloid source that comprises psychoactive fungus, by:

drying and pulverizing the psychoactive alkaloid source to obtain a dried biomass;

placing the dried biomass in a first extraction solvent at a temperature of 5-95° C. for a duration of 10 minutes to 12 hours to obtain a first slurry, wherein the first extraction solvent is a primary aliphatic alcohol, a ketone, purified water or any combination selected therefrom;

filtering the first slurry to obtain a first filtrate and a first residue;

placing the first residue in a second extraction solvent at a temperature of 5-95° C. for a duration of 10 minutes to 12 hours to obtain a second slurry, wherein the second extraction solvent is a primary aliphatic alcohol, a ketone, purified water or any combination selected therefrom;

filtering the second slurry to obtain a second filtrate and a second residue; and

mixing the first filtrate and the second filtrate to obtain the psychoactive alkaloid extract.

16 . The process of claim 1 , wherein the psychoactive alkaloid extract is from Psilocybe fungus.

17 . A powdered composition consisting essentially of:

an extract from one or more mushrooms, the extract comprising psychoactive alkaloid that is psilocybin, psilocin, baeocystin, norbaeocystin, norpsilocin, aeruginascin, bufotenin, bufotenidine, 5-MeO-DMT (5-methoxy-N, N-dimethyltryptamine), N,N-dimethyltryptamine (DMT), ergine (LSA), ergonovine, ergometrine, muscimol, ibotenic acid, lysergic acid hydroxyethylamide (LSH), elymoclavine, ergometrinine, chanoclavine or any combination selected therefrom; and

an excipient;

wherein the psychoactive alkaloid has a concentration between a lower limit of exactly 10.5% and an upper limit of 99% by weight in the powdered composition.

18 . The powdered composition of claim 17 , wherein the excipient comprises ascorbic acid, silicon dioxide, maltodextrin, gum arabic, microcrystalline cellulose, sodium citrate, sodium benzoate, sodium phosphate, rice, rice hulls, or any combination selected therefrom.

19 . The powdered composition of claim 17 , wherein the concentration of the psychoactive alkaloid is specified as a percentage with a precision of two decimal places.

20 . The powdered composition of claim 17 , wherein said one or more mushrooms are Psilocybe cubensis, Psilocybe cyanescens , or both Psilocybe cubensis and Psilocybe cyanescens.

21 . The powdered composition of claim 17 , wherein the extract comprises psilocybin, psilocin, baeocystin, norbaeocystin, or any combination selected therefrom.

22 . The process of claim 1 , wherein the primary aliphatic alcohol in the psychoactive alkaloid extract in liquid or slurry form is a C1-4 alcohol.

23 . The process of claim 1 , wherein the ketone in the psychoactive alkaloid extract in liquid or slurry form is a C3-4 ketone.

24 . The process of claim 8 , wherein the specific concentration is 0.1-68% by weight.

25 . The process of claim 8 , wherein the specific concentration is 5-68% by weight.

26 . The process of claim 8 , wherein the specific concentration is 0.1-54% by weight.

27 . The process of claim 8 , wherein the specific concentration is 0.1-16.7% by weight.