Imaging Analyzer For Testing Analytes
The invention provides analyzers that improve tests for detecting specific cellular, viral, and molecular targets in clinical, industrial, or environmental samples. The invention permits efficient and specific selection and sensitive imaging detection of individual microscopic targets at low magnification. Automated embodiments allow efficient walk-away, on-demand, random-access high-throughput testing. The analyzers perform tests without requiring wash steps thus streamlining engineering and lowering costs. Thus, the invention provides analyzers that can deliver rapid, accurate, and quantitative, easy-to-use, and cost-effective tests for analytes.
1 . An imaging analyzer comprising:
a) a housing that accepts a sample container, wherein said sample container contains a sample and comprises a detection area having a shortest linear dimension of ≥1 mm for detection of a target potentially in said sample;
b) a component for applying a selective force to said sample container;
c) a photoelectric array detector disposed for large area imaging of the detection area; and
d) imaging optics that magnify less than 5 fold.
2 . The analyzer of claim 1 wherein said selective force moves a magnetic particles with an average diameter of less than 0.5 mm and an average density of less than 2 g/cm 3 n through a liquid in said sample container that is held a fixed position in said analyzer over distance of greater than 5 mm at an average speed of greater than 0.5 mm/min wherein said liquid is essentially equivalent in density and viscosity to saline.
3 . The analyzer of claim 1 wherein said device for applying a selective force comprises a magnet that has a shortest overall linear dimension of greater than 10 mm and magnetization greater than 3.5 kilojoules per cubic meter.
4 . The analyzer of claim 1 wherein said imaging optics magnifies less than 2 fold.
5 . The analyzer of claim 1 wherein said analyzer comprises automatic focusing.
6 . The analyzer of claim 1 wherein said analyzer comprises a mechanism for ensuring a fixed distance between said detector and the surface of said sample container that is proximal to said detector.
7 . The analyzer of claim 1 wherein said analyzer illuminates said sample container.
8 . The analyzer of claim 1 wherein said analyzer comprises light emitting diodes for illuminating said sample container.
9 . The analyzer of claim 1 wherein said analyzer accommodates introduction of said sample containers as single or as multiple units.
10 . The analyzer of claim 1 wherein said analyzer comprises automated liquid transfer devices.
11 . The analyzer of claim 1 wherein said analyzer comprises a mechanism that causes liquid flow inside said sample container.
12 . The analyzer of claim 1 wherein said analyzer comprises a robotic gantry that can move a sample container between locations on said analyzer.
13 . The analyzer of claim 1 wherein said analyzer comprises a carousel mechanism that can move a sample container between locations on said analyzer.
14 . The analyzer of claim 1 wherein said analyzer comprises a mechanical track mechanism that can move a sample container between locations on said analyzer.
15 . The analyzer of claim 1 wherein said analyzer comprises a bar code reader.
16 . The analyzer of claim 1 wherein said analyzer comprises an incubator that accommodates said sample containers in an enclosure that stably maintains and average temperature within 2 degrees Celsius of a temperature set point.
17 . The analyzer of claim 1 wherein said analyzer comprises a printer, electronic monitor, and/or system for connections to an external communication network.
18 . The analyzer of claim 1 wherein said analyzer comprises means for automatic cleaning sample containers for reuse in said analyzer.
19 . The analyzer of claim 1 wherein said analyzer comprises one or more receptacles that accept sample containers after imaging on said analyzer.
20 . The analyzer of claim 1 wherein said analyzer comprises one or more receptacles for waste liquids.
21 . The analyzer of claim 1 wherein said analyzer comprises integrated image analysis software with object finding algorithms.
22 . The analyzer of claim 1 wherein said analyzer comprises integrated scheduling software for managing the movement of one or more sample containers between different locations in said analyzer.
23 . The analyzer of claim 1 wherein said analyzer accommodates a sample container that has height greater than 8 mm.
24 . The analyzer, as shown in the Figures.