METHOD FOR PRODUCING CHROMATOGRAPHY CARRIER, METHOD FOR PRODUCING CHROMATOGRAPHY COLUMN, AND CHROMATOGRAPHY CARRIER
A chromatography carrier may exhibit high liquid permeability and an excellent pressure-resistant characteristic during liquid passage. A chromatography carrier production method may include: (1) providing a solid phase support, the solid phase support being formed of porous particles on which a ligand has or has not been immobilized; and (2) subjecting the solid phase support to sieve classification, the coefficient of variation of the volume particle size distribution of the porous particles when a ligand has been immobilized being adjusted to 1% to 22%. The skewness of the volume particle size distribution of the porous particles when a ligand has been immobilized may be adjusted to −0.1 to 5.
1 . A method for producing a chromatography carrier, the method comprising:
subjecting a solid phase support to sieve classification, the solid phase support being formed of porous particles on which a ligand has or has not been immobilized,
wherein a variation coefficient of volume particle size distribution of the porous particles when the ligand has been immobilized is adjusted to be in a range of from 1% to 22%, and
wherein a skewness of volume particle size distribution of the porous particles when the ligand has been immobilized is adjusted to be in a range of from −0.1 to 5.
2 . The method of claim 1 ,
wherein the solid phase support is formed of the porous particles on which the ligand has been immobilized.
3 . The method of claim 1 ,
wherein the solid phase support is formed of porous particles on which the ligand has not been immobilized.
4 . The method of claim 3 , further comprising:
immobilizing the ligand onto the porous particles which have undergone the sieve classification.
5 . method of claim 1 , wherein the sieve classification further comprises:
(2a) performing a sieve classification with a first sieve having a mesh opening of 10% or greater and smaller than 200%, with respect to a mean volume diameter of the solid phase support before sieve classification (2α-d) as 100%, to thereby recover a residue over a sieve;
(2b) performing a sieve classification of the residue with a second sieve having a mesh opening greater than that of the first sieve of the sieve classification (2a) and smaller than 600% of a particle size of the solid phase support before the sieve classification (2α-d), to thereby recover a matter having passed through the second sieve,
wherein the sieve classification (2a) and the sieve classification (2b) are performed in that order or in reverse order.
6 . The method of claim 1 , wherein the sieve classification is a wet process.
7 . The method of claim 1 , wherein the variation coefficient is in a range of from 5% to 20%, and
wherein the skewness is in a range of from 0.01 to 3.
8 . A chromatography carrier production method of claim 1 , further comprising:
dispersing a monomer composition in an aqueous medium and conducting suspension polymerization prior to the subjecting.
9 . A method for producing a chromatography column, the method comprising:
charging a carrier into a column vessel,
wherein the carrier is formed of porous particles onto which a ligand has been immobilized,
wherein a variation coefficient of volume particle size distribution of the carrier is in a range of from 1% to 22%, and
wherein a skewness of volume particle size distribution of the carrier is in a range of from −0.1 to 5.
10 . A chromatography carrier, which is dispersed in a dispersion medium, wherein the carrier is formed of porous particles onto which a ligand has been immobilized,
wherein a variation coefficient of volume particle size distribution of the carrier is in a range of from 1% to 22%, and
wherein a skewness of volume particle size distribution of the carrier is in a range of from −0.1 to 5.
11 . The chromatography carrier of claim 10 , wherein the carrier has a volume cumulative 50% particle size d50 in a range of from 55 μm to 100 μm.
12 . The chromatography carrier of claim 10 , wherein the carrier has a volume cumulative 1% particle size d1 in a range of from 30 μm to 75 μm.
13 . The method of claim 2 , wherein the solid phase support is a synthetic polymer porous particle onto which the ligand has been immobilized.
14 . The method of claim 3 , wherein the solid phase support is a synthetic polymer porous particle onto which the ligand has not been immobilized.
15 . The method of claim 5 , wherein the mesh opening is of 62% or greater and 90% or less of the particle size of the solid phase support before the sieve classification (2α-d).
16 . The method of claim 1 , wherein the variation coefficient is in a range of from 8% to 18%, and
wherein the skewness is in a range of from 0.1 to 3.
17 . The method of claim 1 , wherein, in the performing, the sieve classification is performed so that volume cumulative 1% particle size d1 is adjusted to be in a range of from 35 μm to 65 μm.
18 . The method of claim 1 , wherein, in the performing, the sieve classification is performed so that volume cumulative 5% particle size d1 is adjusted to be in a range of from 40 μm to 65 μm.
19 . The method of claim 1 , wherein, in the performing, the sieve classification is performed so that the ratio (d5/d50) of volume cumulative 5% particle size d5 to volume cumulative 50% particle size d50 is in a range of from 0.70 to 0.95.