METHOD FOR PROVIDING PROTEINACEOUS COMPOSITION
A protein-rich composition can be provided from egg albumen via a process that involves diafiltering with purified water an egg albumen concentrate. Collected retentate from the diafiltration step constitutes a protein-rich composition which, on a dry basis, can include less than 1% (w/w) lipids. The composition can be spray dried to a protein isolate solid which is substantially free of sugars.
1 . A method for providing a protein-enriched composition from egg albumen, the method comprising:
a) providing an egg albumen concentrate by removing at least 25% (v/v) of water from the egg albumen;
b) diafiltering the egg albumen concentrate; and
c) providing a protein-enriched composition by collecting a retentate from step (b),
wherein the egg albumen concentrate of steps (a) and (b) and the protein-enriched composition of step (c) are not pH-adjusted prior to subsequent processing.
2 . The method of claim 1 , wherein at least 33% (v/v) of water is removed from the egg albumen.
3 . The method of claim 1 , wherein water is removed from the egg albumen using reverse osmosis.
4 . The method of claim 1 , wherein the egg albumen concentrate has a protein content of greater than or equal to 15%.
5 . The method of claim 4 , wherein the egg albumen concentrate has a protein content of greater than or equal to 20%.
6 . The method of claim 1 , wherein the egg albumen concentrate is diafiltered with purified water and an ultrafiltration membrane having a nominal molecular weight cutoff of greater than or equal to 7 kDa.
7 . The method of claim 1 , wherein the diafiltration is performed with from about 1 to about 7.5 volumes of diafiltration buffer relative to the volume of the egg albumen concentrate.
8 . The method of claim 1 , wherein the retentate has an electrical conductance of less than or equal to 5 mS.
9 . The method of claim 8 , wherein the retentate has an electrical conductance of less than or equal to 3 mS.
10 . The method of claim 1 , wherein the protein-enriched composition includes, on a dry basis, less than 1% (w/w) lipids.
11 . The method of claim 1 , wherein the protein-enriched composition includes, on a dry basis, less than 0.5% (w/w) lipids.
12 . The method of claim 1 , wherein the protein-enriched composition is substantially free of sugars.
13 . The method of claim 12 , wherein the protein-enriched composition that is substantially free of sugars is provided without the use of fermentative organisms or enzymatic de-sugaring agents.
14 . The method of claim 1 , further comprising pasteurizing the protein-enriched composition.
15 . The method of claim 14 , further comprising providing a solid protein-enriched composition by spray drying the protein-enriched composition.
16 . The method of claim 14 , further comprising providing a solid protein-enriched composition by pulse combustion drying the protein-enriched composition.
17 . The method of claim 14 , further comprising providing a solid protein-enriched composition by spray drying the protein-enriched composition.
18 . The method of claim 17 , further comprising pasteurizing the solid protein-enriched composition.
19 . The method of claim 17 , wherein the solid protein-enriched composition is a free-flowing powder.
20 . The method of claim 17 , further comprising instantizing the powder.