BACTERIOPHAGE COMPOSITIONS FOR TREATING PSEUDOMONAS INFECTION
The present disclosure relates to bacteriophages and compositions capable of infecting and killing Pseudomonas , and use of the same for treating Pseudomonas , e.g. Pseudomonas aeruginosa , bacterial infections.
1 - 205 . (canceled)
206 . A bacteriophage composition comprising one or more bacteriophages comprising a polynucleotide sequence selected from a polynucleotide sequence with at least 90% identity to SEQ ID NO:1, a polynucleotide sequence with at least 90% identity to SEQ ID NO:2, a polynucleotide sequence with at least 90% identity to SEQ ID NO:3, a polynucleotide sequence with at least 90% identity to SEQ ID NO:4, a polynucleotide sequence with at least 90% identity to SEQ ID NO:5, and/or a polynucleotide sequence with at least 90% identity to SEQ ID NO:6; wherein the composition comprises between 1×10 8 and 1×10 11 plaque-forming units (PFU) per milliliter of each bacteriophage.
207 . The bacteriophage composition of claim 206 , further comprising at least one additional bacteriophage comprising a nucleic acid sequence having 90%-100% identity to a sequence selected from: a polynucleotide sequence of SEQ ID NO:7, a polynucleotide sequence of SEQ ID NO:8, a polynucleotide sequence of SEQ ID NO:9, a polynucleotide sequence of SEQ ID NO:10, a polynucleotide sequence of SEQ ID NO:11, a polynucleotide sequence of SEQ ID NO:12, a polynucleotide sequence of SEQ ID NO:13, a polynucleotide sequence of SEQ ID NO:14, a polynucleotide sequence of SEQ ID NO:15, a polynucleotide sequence of SEQ ID NO:16, a polynucleotide sequence of SEQ ID NO:17, a polynucleotide sequence of SEQ ID NO:18, a polynucleotide sequence of SEQ ID NO:19, a polynucleotide sequence of SEQ ID NO:20, a polynucleotide sequence of SEQ ID NO:21, and a polynucleotide sequence of SEQ ID NO:22.
208 . The bacteriophage composition of claim 207 , wherein the at least one additional bacteriophage comprises a polynucleotide sequence with at least 90% identity to SEQ ID NO:7, a polynucleotide with at least 93% identity to SEQ ID NO:8, a polynucleotide sequence with at least 93% identity to SEQ ID NO:9, a polynucleotide sequence with at least 89% identity to SEQ ID NO:10, a polynucleotide sequence with at least 95% identity to SEQ ID NO:11, a polynucleotide sequence with at least 91% identity to SEQ ID NO:12, a polynucleotide sequence with at least 92% identity to SEQ ID NO:13, a polynucleotide sequence with at least 96% identity to SEQ ID NO:14 a polynucleotide sequence with at least 95% identity to SEQ ID NO:15, a polynucleotide sequence with at least 90% identity to SEQ ID NO:16, a polynucleotide sequence with at least 92% identity to SEQ ID NO:17, a polynucleotide sequence with at least 95% identity to SEQ ID NO:18, a polynucleotide sequence with at least 96% identity to SEQ ID NO:19, a polynucleotide sequence with at least 96% identity to SEQ ID NO:20, a polynucleotide sequence with at least 96% identity to SEQ ID NO:21, or a polynucleotide sequence with at least 95% identity to SEQ ID NO:22.
209 . The bacteriophage composition of claim 206 , wherein the one or more bacteriophages comprises a polynucleotide sequence with at least 95% identity to SEQ ID NO:1, a polynucleotide sequence with at least 95% identity to SEQ ID NO:2, a polynucleotide sequence with at least 95% identity to SEQ ID NO:3, a polynucleotide sequence with at least 95% identity to SEQ ID NO:4, a polynucleotide sequence with at least 95% identity to SEQ ID NO:5, and/or a polynucleotide sequence with at least 95% identity to SEQ ID NO:6.
210 . The bacteriophage composition of claim 206 , wherein the one or more bacteriophages comprises the polynucleotide sequence of SEQ ID NO:1, the polynucleotide sequence of SEQ ID NO:2, the polynucleotide sequence of SEQ ID NO:3, the polynucleotide sequence of SEQ ID NO:4, the polynucleotide sequence of SEQ ID NO:5, and/or the polynucleotide sequence of SEQ ID NO:6.
211 . The bacteriophage composition of claim 206 comprising two or more bacteriophages.
212 . The bacteriophage composition of claim 206 comprising three or more bacteriophages.
213 . The bacteriophage composition of claim 206 comprising four or more bacteriophages.
214 . The bacteriophage composition of claim 206 , wherein the one or more bacteriophages target one or more of Pseudomonas aeruginosa , antibiotic-resistant Pseudomonas aeruginosa , and multiple antibiotic-resistant Pseudomonas aeruginosa.
215 . The bacteriophage composition of claim 206 , further comprising a storage medium for storage at room temperature or a temperature at or below 8° C.
216 . The bacteriophage composition of claim 215 , wherein the storage medium comprises a cryoprotectant.
217 . The bacteriophage composition of claim 2011 , wherein the composition's target bacteria range is broader than the cumulative range of the individual bacteriophages in the composition.
218 . The bacteriophage composition of claim 217 , wherein the target bacteria range is broadened within a bacterial species that the bacteriophage is able to infect.
219 . The composition of claim 206 , wherein the composition further comprises a pharmaceutically acceptable carrier, diluent, excipient or combinations thereof.
220 . The composition of claim 219 , wherein the composition is a liquid, semi-liquid, solid, frozen, freeze-dried, cryodesiccated, or lyophilized formulation.
221 . The composition of claim 206 , wherein the composition is formulated for nebulization.
222 . The composition of claim 206 , wherein the one or more bacteriophages are not naturally occurring.
223 . The composition of claim 206 , wherein the one or more bacteriophages reduce biofilm mass.
224 . The composition of claim 206 , wherein the one or more bacteriophages are resistant to sputum inactivation, remain in the lung and/or bronchoalveolar lavage fluid up to 96 hours after administration, and/or maintain activity in the presence of a pulmonary treatment selected from salbutamol, tobramycin, aztreonam, colistin, inhaled hypertonic saline, and inhaled beta-agonist.
225 . The composition of claim 206 , wherein the sequence of at least one bacteriophage is genetically modified.
226 . The composition of claim 206 , wherein at least one of the bacteriophages infect and kill Pseudomonas aeruginosa.
227 . The composition of claim 206 , wherein one or more of the bacteriophages belong to the family Podoviridae or Myoviridae.
228 . The composition of claim 227 , wherein the one or more bacteriophages belong to the family Podoviridae.
229 . The composition of claim 228 , wherein the one or more bacteriophages belong to the genus Phikmvvirus, Litunavirus, Bruynoghevirus, or Luzseptimavirus.
230 . The composition of claim 227 , wherein the one or more bacteriophages belong to the family Myoviridae.
231 . The composition of claim 230 , wherein the one or more bacteriophages belong to the genus Pbunavirus, Pakpunanavirus, or Nankokuvirus.
232 . The composition of claim 206 , wherein the composition is substantially free of a bacterial component and wherein the bacterial component comprises bacterial host protein and/or exotoxin.