Compositions for use in treatment of
This invention relates to compositions (e.g., vaccine compositions) which can be used to immunise against Chlamydia infections. The compositions comprise Chlamydia sp. antigens and antigen combinations which can be used to immunise against Chlamydia sp., used in the form of nucleic acids (e.g., mRNAs) encoding antigenic proteins or in the form of recombinant protein antigens.
1. A composition comprising:
(i) a nucleic acid comprising a nucleotide sequence encoding a modified Major Outer Membrane Protein (MOMP) polypeptide, wherein the modified MOMP polypeptide has an amino acid sequence comprising two or more conserved domain sequences of a native Chlamydia trachomatis MOMP polypeptide and a non-native loop sequence between the conserved domain sequences;
(ii) a nucleic acid that comprises a nucleotide sequence encoding a chimeric Chlamydia trachomatis MOMP variable domain (VD) polypeptide, wherein the chimeric MOMP VD polypeptide comprises an amino acid sequence comprising two or more Chlamydia trachomatis MOMP VD sequences of different serovars of Chlamydia trachomatis;
(iii) a nucleic acid that comprises a nucleotide sequence encoding a Chlamydia trachomatis CT443 polypeptide; and
(iv) a nucleic acid that comprises a nucleotide sequence encoding a Chlamydia trachomatis CT584 polypeptide.
2. A nucleic acid comprising a nucleotide sequence encoding a modified Major Outer Membrane Protein (MOMP) polypeptide, wherein the modified MOMP polypeptide has an amino acid sequence comprising two or more conserved domain sequences of a native Chlamydia sp. MOMP polypeptide and a non-native loop sequence between the conserved domain sequences.
3. The nucleic acid of claim 2 , wherein:
(i) the modified MOMP polypeptide does not comprise a native Chlamydia sp. MOMP variable domain between the two or more conserved domain sequences;
(ii) the modified MOMP polypeptide comprises three, four or five conserved domain sequences of a native Chlamydia sp. MOMP polypeptide;
(iii) the modified MOMP polypeptide comprises three, four or five conserved domain sequences of a native Chlamydia sp. MOMP polypeptide and a non-native loop sequence between each of the conserved domain sequences; and/or
(iv) the non-native loop sequence is between 3 and 30 amino acids in length.
4. The nucleic acid of claim 2 , wherein:
(i) a non-native loop sequence comprising a sequence according to SEQ ID NO: 462 or 466 replaces variable domain 1 between the conserved domain sequences;
(ii) a non-native loop sequence comprising a sequence according to SEQ ID NO: 463 or 467 replaces variable domain 2 between the conserved domain sequences;
(iii) a non-native loop sequence comprising a sequence according to SEQ ID NO: 464 or 468 replaces variable domain 3 between the conserved domain sequences; and/or
(iv) a non-native loop sequence comprising a sequence according to SEQ ID NO: 465 or 469 replaces variable domain 4 between the conserved domain sequences.
5. The nucleic acid of claim 2 , wherein:
(i) the modified MOMP polypeptide comprises a sequence according to any one of SEQ ID NOs: 486-489 or a sequence that has at least 70% identity thereto;
(ii) the modified MOMP polypeptide further comprises a secretion signal peptide sequence; and/or
(iii) the nucleic acid comprises a nucleotide sequence according to any one of SEQ ID NOs: 551-566 or a sequence that has at least 50% identity thereto.
6. A modified Major Outer Membrane Protein (MOMP) polypeptide having an amino acid sequence comprising two or more conserved domain sequences of a native Chlamydia sp. MOMP polypeptide and a non-native loop sequence between the conserved domain sequences.
7. The modified MOMP polypeptide of claim 6 , wherein:
(i) the modified MOMP polypeptide does not comprise a native Chlamydia sp. MOMP variable domain between the two or more conserved domain sequences;
(ii) the modified MOMP polypeptide comprises three, four or five conserved domain sequences of a native Chlamydia sp. MOMP polypeptide;
(iii) the modified MOMP polypeptide comprises three, four or five conserved domain sequences of a native Chlamydia sp. MOMP polypeptide and a non-native loop sequence between each of the conserved domain sequences; and/or
(iv) the non-native loop sequence is between 3 and 30 amino acids in length.
8. The modified MOMP polypeptide of claim 6 , wherein:
(i) a non-native loop sequence comprising a sequence according to SEQ ID NO: 462 or 466 replaces variable domain 1 between the conserved domain sequences;
(ii) a non-native loop sequence comprising a sequence according to SEQ ID NO: 463 or 467 replaces variable domain 2 between the conserved domain sequences;
(iii) a non-native loop sequence comprising a sequence according to SEQ ID NO: 464 or 468 replaces variable domain 3 between the conserved domain sequences; and/or
(iv) a non-native loop sequence replacing VD4 comprises a sequence according to SEQ ID NO: 465 or 469 replaces variable domain 4 between the conserved domain sequences.
9. The modified MOMP polypeptide of claim 6 , wherein the modified MOMP polypeptide comprises a sequence according to any one of SEQ ID NOs: 486-489 or a sequence that has at least 70% identity thereto.
10. A composition comprising the nucleic acid of claim 2 .
11. A composition comprising the modified MOMP polypeptide of claim 6 .
12. A method of treating or preventing at least one symptom of a Chlamydia sp. infection in a subject, the method comprising administering the composition of claim 1 to the subject, wherein the infection is a C. trachomatis infection, and wherein the nucleic acid of (i), the nucleic acid of (ii), the nucleic acid of (iii) and the nucleic acid of (iv) are each expressed in the subject.
13. A method of treating or preventing at least one symptom of a Chlamydia sp. infection in a subject, the method comprising administering the modified MOMP polypeptide of claim 6 to the subject, wherein the infection is a C. trachomatis infection.
14. The composition of claim 1 , wherein one or more nucleic acids is a mRNA.
15. The composition of claim 14 , wherein:
(i) the mRNA comprises at least one 5′ untranslated region (5′ UTR), at least one 3′ untranslated region (3′ UTR), and/or at least one polyadenylation (poly(A)) sequence; and/or
(ii) the mRNA is chemically modified and wherein the chemical modification consists of N 1 -methylpseudouridine in place of every uridine.
16. The composition of claim 1 , wherein the composition further comprises a lipid nanoparticle (LNP).
17. The composition of claim 16 , wherein:
(i) the LNP comprises at least one cationic lipid, wherein the cationic lipid is selected from the group consisting of OF-02, cKK-E10, OF-Deg-Lin, GL-HEPES-E3-E10-DS-3-E18-1, GL-HEPES-E3-E12-DS-4-E10, GL-HEPES-E3-E12-DS-3-E14, SM-102, ALC-0315, ATX-126, and IM-001; and/or
(ii) the LNP comprises a polyethylene glycol (PEG) conjugated (PEGylated) lipid, a cholesterol-based lipid, and a helper lipid.
18. The composition of claim 1 , wherein:
(i) the nucleic acid that comprises a nucleotide sequence encoding a Chlamydia trachomatis modified Major Outer Membrane Protein (MOMP) polypeptide is a mRNA which comprises the following structural elements:
a 5′ cap;
a 5′ untranslated region (5′ UTR);
a nucleotide sequence encoding the modified Major Outer Membrane Protein (MOMP) polypeptide comprising the sequence according to SEQ ID NO: 486;
a 3′ untranslated region (3′ UTR); and
a polyA tail;
(ii) the nucleic acid comprising a nucleotide sequence encoding the chimeric Chlamydia trachomatis MOMP VD polypeptide is a mRNA which comprises the following structural elements:
a 5′ cap;
a 5′ untranslated region (5′ UTR);
a nucleotide sequence encoding the chimeric Chlamydia trachomatis MOMP VD polypeptide comprising the sequence according to SEQ ID NO: 503;
a 3′ untranslated region (3′ UTR); and
a polyA tail;
(iii) the nucleic acid that comprises a nucleotide sequence encoding a Chlamydia trachomatis CT443 polypeptide is a mRNA which comprises the following structural elements:
a 5′ cap;
a 5′ untranslated region (5′ UTR);
a nucleotide sequence encoding the Chlamydia trachomatis CT443 polypeptide comprising the sequence according to SEQ ID NO 507;
a 3′ untranslated region (3′ UTR); and
a polyA tail; and
(iv) the nucleic acid that comprises a nucleotide sequence encoding a Chlamydia trachomatis CT584 polypeptide is a mRNA which comprises the following structural elements:
a 5′ cap;
a 5′ untranslated region (5′ UTR);
a nucleotide sequence encoding the Chlamydia trachomatis CT584 polypeptide comprising the sequence according to SEQ ID NO: 510;
a 3′ untranslated region (3′ UTR); and
a polyA tail;
wherein the mRNA is chemically modified and wherein the chemical modification consists of N 1 -methylpseudouridine in place of every uridine.
19. The composition of claim 1 , wherein the composition is immunogenic.
20. The nucleic acid of claim 2 , wherein the Chlamydia sp. is Chlamydia trachomatis.
21. The nucleic acid of claim 3 , wherein:
(i) the modified MOMP polypeptide comprises all five full-length conserved domains of a native Chlamydia sp. MOMP polypeptide;
(ii) the conserved domains of the modified MOMP polypeptide collectively have at least 95% sequence identity to the conserved domains of a native MOMP polypeptide;
(iii) the modified MOMP polypeptide comprises four non-native loop sequences and does not comprise any native Chlamydia sp. MOMP variable domains between the conserved domain sequences; and/or
(iv) the non-native loop sequence is between 4 and 20 amino acids in length.
22. The nucleic acid of claim 2 , wherein the nucleic acid is a mRNA.
23. The nucleic acid of claim 22 , wherein:
(i) the mRNA comprises at least one 5′ untranslated region (5′ UTR), at least one 3′ untranslated region (3′ UTR), and/or at least one polyadenylation (poly(A)) sequence; and/or
(ii) the mRNA is chemically modified and wherein the chemical modification consists of N 1 -methylpseudouridine in place of every uridine.
24. The modified MOMP polypeptide of claim 6 , wherein the Chlamydia sp. is Chlamydia trachomatis.
25. The modified MOMP polypeptide of claim 7 , wherein:
(i) the modified MOMP polypeptide comprises all five full-length conserved domains of a native Chlamydia sp. MOMP polypeptide;
(ii) the conserved domains of the modified MOMP polypeptide collectively have at least 95% sequence identity to the conserved domains of a native MOMP polypeptide;
(iii) the modified MOMP polypeptide comprises four non-native loop sequences and does not comprise any native Chlamydia sp. MOMP variable domains between the conserved domain sequences; and/or
(iv) the non-native loop sequence is between 4 and 20 amino acids in length.
26. The composition of claim 10 , wherein the composition further comprises a lipid nanoparticle (LNP).
27. The composition of claim 26 , wherein:
(i) the LNP comprises at least one cationic lipid, wherein the cationic lipid is selected from the group consisting of OF-02, cKK-E10, OF-Deg-Lin, GL-HEPES-E3-E10-DS-3-E18-1, GL-HEPES-E3-E12-DS-4-E10, GL-HEPES-E3-E12-DS-3-E14, SM-102, ALC-0315, ATX-126, and IM-001; and/or
(ii) the LNP comprises a polyethylene glycol (PEG) conjugated (PEGylated) lipid, a cholesterol-based lipid, and a helper lipid.
28. The composition of claim 10 , wherein the composition further comprises:
(i) a nucleic acid that comprises a nucleotide sequence encoding a chimeric Chlamydia sp. MOMP variable domain (VD) polypeptide, wherein the chimeric MOMP VD polypeptide comprises an amino acid sequence comprising two or more Chlamydia sp. MOMP VD sequences of different serovars of the Chlamydia sp.; and/or
(ii) one or more of:
(a) a nucleic acid that comprises a nucleotide sequence encoding a Chlamydia sp. CT443 polypeptide;
(b) a nucleic acid that comprises a nucleotide sequence encoding a Chlamydia sp. CT584 polypeptide;
(c) a nucleic acid that comprises a nucleotide sequence encoding a Chlamydia sp. CT600 polypeptide; and
(d) a nucleic acid that comprises a nucleotide sequence encoding a Chlamydia sp. CT812 polypeptide.
29. The composition of claim 11 , wherein the composition further comprises:
(i) a chimeric Chlamydia sp. MOMP variable domain (VD) polypeptide, wherein the chimeric MOMP VD polypeptide comprises an amino acid sequence comprising two or more Chlamydia sp. MOMP VD sequences of different serovars of the Chlamydia sp.; and/or
(ii) one or more of:
(a) a polypeptide comprising amino acid sequence of a Chlamydia sp. CT443 polypeptide;
(b) a polypeptide comprising amino acid sequence of a Chlamydia sp. CT584 polypeptide;
(c) a polypeptide comprising amino acid sequence of a Chlamydia sp. CT600 polypeptide; or
(d) a polypeptide comprising amino acid sequence of a Chlamydia sp. CT812 polypeptide.
30. A method of treating or preventing at least one symptom of a Chlamydia sp. infection in a subject, the method comprising administering the composition of claim 10 to the subject, wherein the infection is a C. trachomatis infection, and wherein the nucleic acid is expressed in the subject.
31. The method of claim 12 , wherein the composition further comprises a lipid nanoparticle (LNP).
32. The method of claim 31 , wherein the nucleic acid is encapsulated in the LNP.
33. The method of claim 30 , wherein the composition further comprises a lipid nanoparticle (LNP).
34. The method of claim 33 , wherein the nucleic acid is encapsulated in the LNP.
35. A method of generating an immune response against a Chlamydia sp. infection in a subject, the method comprising administering the composition of claim 1 to the subject, wherein the infection is a C. trachomatis infection, and wherein the nucleic acid of (i), the nucleic acid of (ii), the nucleic acid of (iii), and the nucleic acid of (iv) are each expressed in the subject.
36. A method of generating an immune response against a Chlamydia sp. infection in a subject, the method comprising administering the modified MOMP polypeptide of claim 6 to the subject, wherein the infection is a C. trachomatis infection.
37. A method of generating an immune response against a Chlamydia sp. infection in a subject, the method comprising administering the composition of claim 10 to the subject, wherein the infection is a C. trachomatis infection, and wherein the nucleic acid is expressed in the subject.
38. The composition of claim 1 , wherein:
(i) the modified MOMP polypeptide comprises the sequence according to SEQ ID NO: 486;
(ii) the chimeric Chlamydia trachomatis MOMP VD polypeptide comprises the sequence according to SEQ ID NO: 503;
(iii) the Chlamydia trachomatis CT443 polypeptide comprises the sequence according to SEQ ID NO 507; and
(iv) the Chlamydia trachomatis CT584 polypeptide comprises the sequence according to SEQ ID NO: 510.