MODIFIED POLYMER FILM SURFACES FOR SINGLE-USE BIOREACTOR BAGS AND BIOCONTAINMENT AND METHODS OF FORMING SAME
In some embodiments, a polymer film includes a base composition of poly(ethylene-vinyl acetate) and a surface composition comprising hydroxy groups. In some embodiments, a polymer film includes a base layer of a first composition of poly(ethylene-vinyl acetate), a surface layer at a surface of the base layer, and a coating layer of a second composition of a copolymer of glycerol and sebacic acid. The surface layer includes surface hydroxy groups converted from acetate groups of the poly(ethylene-vinyl acetate). The second composition is attached to the surface layer by ester bonds between carboxyl groups of the copolymer and the hydroxy groups. A single-use bioreactor bag includes a polymer film including a base composition of poly(ethylene-vinyl acetate) and a surface composition comprising hydroxy groups. A method of modifying a poly(ethylene-vinyl acetate) film includes converting acetate groups at a first surface of the poly(ethylene-vinyl acetate) film to hydroxy groups.
1 . A polymer film comprising:
a base layer of poly(ethylene-vinyl acetate); and
a surface layer at a surface of the base layer, the surface layer comprising surface hydroxy groups converted from acetate groups of the poly(ethylene-vinyl acetate).
2 . The polymer film of claim 1 further comprising:
a coating layer of a copolymer of glycerol and sebacic acid;
wherein the coating layer is attached to the surface layer by ester bonds between carboxyl groups of the copolymer of glycerol and sebacic acid and the hydroxy groups.
3 . The polymer film of claim 2 , wherein the copolymer of glycerol and sebacic acid is poly(glycerol sebacate).
4 . The polymer film of claim 2 , wherein the copolymer of glycerol and sebacic acid is poly(glycerol sebacate urethane).
5 . The polymer film of claim 2 , wherein the copolymer of glycerol and sebacic acid is poly(glycerol sebacate) acrylate.
6 . The polymer film of claim 2 , wherein the coating layer further comprises a short chain fatty acid.
7 . A single-use bioreactor bag comprising a polymer film comprising a base layer of poly(ethylene-vinyl acetate) and a surface layer at a surface of the base layer, the surface layer comprising surface hydroxy groups converted from acetate groups of the poly(ethylene-vinyl acetate).
8 . The single-use bioreactor bag of claim 7 , wherein the surface layer is on a single side of the polymer film.
9 . The single-use bioreactor bag of claim 7 , wherein the surface layer is on a first side of the polymer film and a second side of the polymer film opposite the first side.
10 . The single-use bioreactor bag of claim 7 , wherein the surface layer extends a conversion depth of up to about 1 mil into the polymer film.
11 . The single-use bioreactor bag of claim 7 further comprising a surface layer cured to the surface composition, the surface layer comprising a copolymer of glycerol and sebacic acid.
12 . The single-use bioreactor bag of claim 11 , wherein the surface layer is an inner surface of the single-use bioreactor bag.
13 . The single-use bioreactor bag of claim 7 , wherein the single-use bioreactor bag is a blood bag.
14 . The single-use bioreactor bag of claim 7 , wherein the single-use bioreactor bag is a cryopreservation bag.
15 . A method of modifying a poly(ethylene-vinyl acetate) film, the method comprising:
converting acetate groups at a first surface of the poly(ethylene-vinyl acetate) film to hydroxy groups.
16 . The method of claim 15 , wherein the converting comprises a process selected from the group consisting of saponification, radiation oxidation, plasma treatment, laser ablation, and laser oxidation.
17 . The method of claim 15 , wherein the converting converts acetate groups to hydroxy groups to a conversion depth of up to about 1 mil into the polymer film.
18 . The method of claim 15 , wherein the converting also converts acetate groups at a second surface of the polyvinyl acetate film opposite the first surface to hydroxy groups.
19 . The method of claim 15 further comprising applying a coating comprising an alcohol-reactable polymer to the first surface.
20 . The method of claim 19 , wherein the applying comprises spraying the coating on the first surface.
21 . The method of claim 19 , wherein the applying comprises dip coating the coating on the first surface.
22 . The method of claim 19 further comprising curing the coating to the first surface to covalently attach the coating to the first surface.
23 . The method of claim 22 , wherein the coating comprises a copolymer of glycerol and sebacic acid.