Conjugates And Conjugating Reagents
The invention relates to a conjugate of a protein or peptide with a therapeutic, diagnostic or labelling agent, said conjugate containing a protein or peptide bonding portion and a polyethylene glycol portion; in which said protein or peptide bonding portion has the general formula: in which Pr represents said protein or peptide, each Nu represents a nucleophile present in or attached to the protein or peptide, each of A and B independently represents a C 1-4 alkylene or alkenylene chain, and W′ represents an electron withdrawing group or a group obtained by reduction of an electron withdrawing group; and in which said polyethylene glycol portion is or includes a pendant polyethylene glycol chain which has a terminal end group of formula —CH 2 CH 2 OR in which R represents a hydrogen atom, an alkyl group, or an optionally substituted aryl group. Also claimed are a method for making such a conjugate, and novel reagents useful in that method.
1 - 24 . (canceled)
25 . A conjugate of an antibody or antibody fragment with a therapeutic, diagnostic or labelling agent, said conjugate being a conjugate formed from the conjugation of an antibody or antibody fragment with a conjugating reagent selected from conjugation reagent 1:
conjugation reagent 5:
conjugation reagent 8:
conjugation reagent 9:
conjugation reagent 11A:
conjugation reagent 13:
conjugation reagent 16A:
conjugation reagent 20:
conjugation reagent 21:
and
conjugation reagent 35:
26 . The conjugate of claim 25 , wherein the conjugation reagent is conjugation reagent 9.
27 . The conjugate of claim 26 , wherein the antibody or antibody fragment is brentuximab.