IP Library › Granted Patent US 12,338,427
Granted Patent B2
US 12,338,427 · App. 18/628,247 · Granted Jun 24, 2025

Distributed systems for the efficient production and use of microbe-based compositions

Inventors: Sean Farmer (Ft. Lauderdale, FL); Paul S. Zorner (Encinitas, CA); Ken Alibek (Solon, OH); Kent Adams (Oro Valley, AZ); Tyler Dixon (Madison, OH)
Assignee: LOCUS SOLUTIONS IPCO, LLC
C12M41/26C12M23/44C12M23/52C12M23/58C12M41/12C12N1/14C12N1/16C12N1/20C12P7/64C12P19/44Y02A40/10
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Quick Facts
Patent No.
US 12,338,427
App. No.
18/628,247
Granted
Jun 24, 2025
Kind
B2
Abstract

The invention relates to systems and methods for effective production and use of microorganisms and/or the fermentation broth in which they are produced. Advantageously, the system is cost-effective, scalable, quick, versatile, efficacious, and helpful in reducing resistance to chemical compounds and residue that concerns consumers.

Claims (9)

1. A method for improving water flow through soil and/or improving aeration of soil wherein said method comprises applying to the soil a microbe-based product comprising microbes, a broth in which said microbes were grown, and a biosurfactant produced by said microbes; wherein said method further comprises growing the microbes in a microbe growth facility, harvesting said microbes, said broth and said biosurfactant; creating the microbe-based product comprising the harvested microbes, broth and biosurfactant; transporting the microbe-based product to a location for use; and applying the product to the soil;

wherein the biosurfactant is selected from the group consisting of rhamnolipids, sophorolipids (SLP) and mannosylerythritol lipids (MEL); and

wherein said microbes are selected from the group consisting of Pseudomonas aeruginosa, Starmerella bombicola, Pichia anomala, Wickerhamomyces anomalus, Trichoderma harzianum, Bacillus amyloliquefaciens, Bacillus subtilis , and Pseudozyma aphidis.

2. The method, according to claim 1 , wherein a cellular mass of the microbes in the microbe-based product is less than 50% of the total weight of the microbe-based product.

3. The method, according to claim 1 , wherein the microbe-based product comprises Starmerella bombicola.

4. The method of claim 1 , which improves water drainage through the soil.

5. The method of claim 1 , which improves aeration and/or porosity of compacted soil.

6. The method, according to claim 1 , wherein the composition microbe-based product comprises a MEL as a biosurfactant.

7. The method, according to claim 1 , wherein the composition microbe-based product comprises a SLP as a biosurfactant.

Assignments (4)
SECURITY INTEREST Recorded Nov 11, 2024
From: LOCUS SOLUTIONS IPCO, LLC
To: U.S. BANK TRUST COMPANY, NATIONAL ASSOCIATION, AS COLLATERAL AGENT
Reel/Frame 069333/0911 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 12, 2024
From: LOCUS SOLUTIONS, LLC
To: LOCUS IP COMPANY, LLC
Reel/Frame 067084/0557 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 12, 2024
From: LOCUS IP COMPANY, LLC
To: LOCUS SOLUTIONS IPCO, LLC
Reel/Frame 067097/0405 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 11, 2024
From: FARMER, SEAN; ZORNER, PAUL S.; ALIBEK, KEN; ADAMS, KENT; DIXON, TYLER
To: LOCUS SOLUTIONS, LLC
Reel/Frame 067080/0601 →
Continuity (4)
Continuation 18447387 · Aug 10, 2023
Continuation 16331438
Provisional Application 62385057 · Sep 8, 2016
Related Publication 20240271076A1 · Aug 15, 2024
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