Granted Patent
B2
US 12,399,193 · App. 18/652,602 · Granted Aug 26, 2025
Systems, devices, and methods for combined cell processes
Assignee:
Cellares Corporation
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Abstract
The present disclosure relates to systems, devices, and methods for spinoculation and counterflow centrifugal elutriation. In an embodiment, the present disclosure relates to a cartridge in an automated system, comprising a liquid transfer bus, a module fluidically coupled to the liquid transfer bus, the module comprising two sub-modules, each configured to perform separate cell processing steps, and at least one selector valve configured to direct fluid to at least one of the sub-modules.
Claims (35)
1. A cartridge for processing cells in an automated system comprising:
a liquid transfer bus;
a module fluidically coupled to the liquid transfer bus, wherein the module comprises:
a housing comprising a first portion and a second portion configured to couple together to form a cavity;
a spinoculation sub-module; and
an elutriation sub-module, wherein the spinoculation and elutriation sub-modules are enclosed within the cavity formed by the first and second portions of the housing; and
a rotor comprising at least one integrated selector valve configured to direct fluid to at least one of the spinoculation and elutriation sub-modules.
2. The cartridge of claim 1 , wherein the at least one selector valve comprises a first selector valve and a second selector valve.
3. The cartridge of claim 2 , further comprising a third selector valve.
4. The cartridge of claim 3 , wherein the first selector valve is adjustable to define a first fluid flow path, the second selector valve is adjustable to define a second fluid flow path, and the third selector valve is adjustable to define a third fluid flow path.
5. The cartridge of claim 4 , wherein the first and second selector valves are adjustable to direct fluid to a spinoculation chamber of the spinoculation sub-module.
6. The cartridge of claim 4 , wherein the first and second selector valves are adjustable to direct fluid to an elutriation chamber of the elutriation sub-module.
7. The cartridge of claim 4 , wherein the first, second, and third selector valves are adjustable to direct fluid to an elutriation chamber of the elutriation sub-module and fluid to a spinoculation chamber of the spinoculation sub-module in series.
8. The cartridge of claim 1 further comprising a first fluid opening fluidically coupled to the at least one selector valve via at least one fluid channel.
9. The cartridge of claim 8 , wherein the first fluid opening is fluidically coupled to the liquid transfer bus.
10. The cartridge of claim 1 , wherein the spinoculation sub-module and the elutriation sub-module share the rotor.
11. The cartridge of claim 1 , wherein the spinoculation sub-module comprises a spinoculation chamber having a first wall and a second wall that define a gap therebetween.
12. The cartridge of claim 1 , wherein the elutriation sub-module comprises an elutriation chamber having an elutriation cone.
13. The cartridge of claim 1 , wherein the at least one selector valve comprises a detent configured to prevent rotation of the at least one selector valve.
14. The cartridge of claim 1 , wherein the rotor further comprises an optical feature configured to be detected by an optical sensor external to the cartridge.
15. A cartridge for processing cells in an automated system comprising:
a liquid transfer bus; and
a module comprising:
a spinoculation sub-module;
an elutriation sub-module; and
a rotor positioned between and shared by the spinoculation and elutriation sub-modules, wherein the rotor comprises at least one integrated selector valve configured to direct fluid to at least one of the spinoculation and the elutriation sub-modules.
16. The cartridge of claim 15 , wherein the module further comprises a housing, and wherein the rotor, the spinoculation sub-module, and the elutriation sub-module are enclosed within a cavity formed by first and second portions of the housing.
17. The cartridge of claim 16 , wherein the housing comprises at least one viewing window.
18. The cartridge of claim 15 , wherein the at least one selector valve comprises first and second selector valves, each configured to selectively direct fluid to the spinoculation or elutriation sub-module.
19. The cartridge of claim 15 , wherein the module further comprises a seal between the spinoculation sub-module and elutriation sub-module.
20. The cartridge of claim 19 , wherein the seal comprises a first fluid opening and a second fluid opening, and wherein the first fluid opening is in fluid communication with the liquid transfer bus.
21. The cartridge of claim 20 , wherein the second fluid opening is in fluid communication with the at least one selector valve.
22. The cartridge of claim 16 further comprising a magnetic hub for attaching the cartridge to a corresponding instrument within a cell processing workcell.
23. The cartridge of claim 15 , wherein the at least one selector valve is adjustable between different positions to define different fluid flow paths through the rotor.
24. The cartridge of claim 15 , wherein the at least one selector valve comprises a rotary valve.
Assignments (2)
SECURITY INTEREST
Recorded May 5, 2026
From: CELLARES CORPORATION
To: TRINITY CAPITAL INC.
Reel/Frame 075545/0558 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Sep 12, 2024
From: TIAN, ISABEL; TOY, WILSON WAI
To: CELLARES CORPORATION
Reel/Frame 068574/0538 →
Continuity (2)
Provisional Application
63464386
· May 5, 2023
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