METHOD FOR FILLING COLUMN WITH CHROMATOGRAPHY CARRIER, METHOD FOR STORING SLURRY, AND SLURRY
Provided is a method for filling a column with a chromatography carrier, including substituting a slurry with an aqueous solvent not containing a buffer, in which the slurry contains a target substance-capturing chromatography carrier, a buffer having an acid dissociation constant (pKa) within a range of ±1.0 of the isoelectric point of the carrier, and an aqueous solvent and has a pH of a liquid phase adjusted within a range of ±2.0 of the isoelectric point of the carrier, and filling a column with the slurry subjected to solvent substitution. The method may be advantageous for suppressing a decrease in liquid permeability and pressure resistance characteristics during liquid passage of a chromatography carrier when a solvent is substituted with an aqueous solvent not containing a buffer.
1 . A method for filling a column with a chromatography carrier, comprising:
substituting a slurry with an aqueous solvent not containing a buffer, wherein the slurry contains a target substance-capturing chromatography carrier, a buffer having an acid dissociation constant (pKa) within a range of ±1.0 of an isoelectric point of the carrier and an aqueous solvent, and has a pH of a liquid phase adjusted within a range of ±2.0 of the isoelectric point of the carrier; and
filling a column with the slurry subjected to solvent substitution in the substituting.
2 . The method according to claim 1 ,
wherein the pH of the liquid phase of the slurry subjected to solvent substitution in the substituting is in a range of 3 or more and 10 or less.
3 . The method according to claim 1 ,
wherein an electrical conductivity of the liquid phase of the slurry subjected to solvent substitution in the substituting is in a range of 0.006 mS/m or more and 200 mS/m or less.
4 . The method according to claim 1 ,
wherein the carrier is an affinity chromatography carrier or an ion exchange chromatography carrier.
5 . The method according to claim 1 ,
wherein the buffer is a buffer selected from a compound having a plurality of acidic groups in a molecule and a salt thereof.
6 . The method according to claim 1 ,
wherein a content of the buffer is 0.1 mM or more in the liquid phase of the slurry used in the substituting.
7 . A method for storing a slurry which is used by substituting with an aqueous solvent not containing a buffer before filling to a chromatography column,
wherein the slurry contains a target substance-capturing chromatography carrier, a buffer having an acid dissociation constant (pKa) within a range of ±1.0 of an isoelectric point of the carrier, and an aqueous solvent, and
a pH of a liquid phase of the slurry is in a range of ±2.0 of the isoelectric point of the carrier.
8 . A slurry which is used by substituting with an aqueous solvent not containing a buffer before filling to a column, comprising:
a target substance-capturing chromatography carrier;
a buffer having an acid dissociation constant (pKa) within a range of ±1.0 of an isoelectric point of the carrier, and
an aqueous solvent,
wherein a pH of a liquid phase is in a range of ±2.0 of the isoelectric point of the carrier.
9 . The slurry according to claim 8 ,
wherein the buffer is a buffer selected from a compound having a plurality of acidic groups in a molecule and a salt thereof.