COMPOSITIONS HAVING HIGH LEVELS OF DPA (n-3) AND METHODS FOR PRODUCING
Disclosed herein are compositions produced from microorganisms that are capable of producing high levels of docosapentaenoic acid (DPA(n-3)), and methods for producing the compositions. The invention is further directed to microbial strains and derivatives therefrom, biomasses, and microbial oils.
1 . A composition comprising a first microbial oil, wherein the first microbial oil comprises long chain polyunsaturated fatty acids (LC-PUFAs), and wherein the LC-PUFAs comprise docosapentaenoic acid (DPA(n-3)) and docosahexaenoic acid (DHA) in a ratio of DPA(n-3):DHA greater than 0.2.
2 . The composition of claim 1 , wherein the ratio of DPA(n-3):DHA in the first microbial oil is between 0.2:1 and 1:1.
3 . The composition of claim 1 , wherein the ratio of DPA(n-3):DHA in the first microbial oil is between 0.3:1 and 0.8:1.
4 . The composition of any preceding claim , wherein the LC-PUFAs in the first microbial oil comprise DPA(n-3) in an amount at least about 7% by weight of the total fatty acids.
5 . The composition of any preceding claim , wherein the LC-PUFAs in the first microbial oil comprise DPA(n-3) in an amount between about 7% to about 12% by weight of the total fatty acids.
6 . The composition of any preceding claim , wherein the LC-PUFAs in the first microbial oil comprise DPA(n-3) in an amount between about 8% to about 11% by weight of the total fatty acids.
7 . The composition of any preceding claim , wherein the first microbial oil is mixed with a second oil, and wherein the second oil comprises a vegetable, plant, fish, microbial oil, or combination thereof.
8 . The composition of claim 7 , wherein the first microbial oil has a ratio of DHA:DPA(n-3) of 0.2-0.7:1.
9 . The composition of claim 7 or claim 8 , wherein the second oil has a ratio of DHA:DPA(n-3):EPA of 0.01-0.7:1:0.01-0.7.
10 . The composition of any preceding claim wherein the first microbial oil is obtained from a microorganism selected from the group consisting of algae, bacteria, fungi and protists.
11 . The composition of claim 10 , wherein the first microbial oil is obtained from a microorganism of the Ichthyosporea class.
12 . The composition of claim 11 , wherein the microorganism is Sphaeroforma arctica.
13 . The composition of any one of claims 7-12 , wherein the second oil is a microorganism selected from the group consisting of Thraustochytriales, dinoflagellates, and Mortierella.
14 . The composition of claim 13 , wherein the microorganism is selected from the group consisting of Schizochytrium, Thraustochytrium , and Crypthecodinium.
15 . The composition of any preceding claim , wherein the composition is a food product, cosmetic or pharmaceutical composition.
16 . The composition of claim 15 , wherein the composition is an infant formula.
17 . The composition of claim 15 , wherein the composition is a dietary supplement.
18 . The composition of claim 15 , wherein the composition is a beverage, therapeutic drink, a nutritional drink, or combination thereof.
19 . A method of preparing a composition comprising combining nutritional components and LC-PUFAs, wherein the LC-PUFAs comprise DPA(n-3) and DHA, and wherein the ratio of DPA(n-3):DHA is from about 0.2:1 to about 1:1.
20 . The method of claim 19 , wherein the composition is an infant formula.
21 . The method of claim 19 , wherein the composition is a dietary supplement.
22 . The method of claim 19 , wherein the composition is a beverage, therapeutic drink, a nutritional drink, or combination thereof.
23 . A method of preparing a composition, comprising combining nutritional components and an oil blend, wherein the oil blend comprises a first microbial oil having a DHA:DPA(n-3) ratio of 0.2-0.7:1, and a second oil comprising one or more of a vegetable oil, a plant oil, a fish oil, or a microbial oil having a DHA:DPA(n-3):EPA ratio of 0.2-0.7:1:0.2-0.7
24 . The method of claim 23 , wherein the composition is an infant formula.
25 . The method of claim 23 , wherein the composition is a dietary supplement.
26 . The method of claim 23 , wherein the composition is a beverage, therapeutic drink, a nutritional drink, or combination thereof.
27 . A method of feeding an infant comprising administering an infant formula composition of any one of claims 1-18 to an infant.
28 . A composition for consumption by a human, wherein the composition comprises long chain polyunsaturated fatty acids (LC-PUFAs) and wherein, when ready for consumption by the human, the LC-PUFAs comprise docosapentaenoic acid n-3 (DPA(n-3)) and docosahexaenoic acid (DHA) in a ratio of DPA(n-3):DHA greater than 0.2.
29 . The composition of claim 28 , wherein the ratio of DPA(n-3):DHA is between 0.2:1 and 1:1.
30 . The composition of claim 28 , wherein the ratio of DPA(n-3):DHA is between 0.3:1 and 0.8:1.
31 . The composition of any one of claims 28-30 , wherein the LC-PUFAs are supplied in a source oil, wherein the source oil comprises DPA(n-3) in an amount of at least about 7% by weight.
32 . The composition of any one of claims 28-31 , wherein the LC-PUFAs are supplied in a source oil, wherein the source oil comprises DPA(n-3) in an amount between about 7% to about 12% by weight.
33 . The composition of any one of claims 28-32 , wherein the LC-PUFAs are supplied in a source oil, wherein the source oil comprises DPA(n-3) in an amount between about 8% to about 11% by weight.
34 . The composition of any one of claims 28-33 , wherein the ratio of DPA(n-3):DHA greater than 0.2 is obtained by mixing a first plant, fish, or microbial oil, or combinations thereof, with a second microbial oil having a DHA:DPA(n-3):EPA ratio of 0.01-0.7:1:0.01-0.7, optionally having a DHA:DPA(n-3):EPA ratio of 0.2-0.7:1:0.2-0.7.
35 . The composition of claim 34 , wherein the second microbial oil is obtained from a microorganism selected from the group consisting of algae, bacteria, fungi and protists.
36 . The composition of claim 35 , wherein the second microbial oil is obtained from a microorganism of the Ichthyosporea class.
37 . The composition of claim 36 , wherein the microorganism is Sphaeroforma arctica.
38 . The composition of any one of claims 34-37 , wherein the first oil is obtained from a microorganism selected from the group consisting of Thraustochytriales, dinoflagellates, and Mortierella.
39 . The composition of claim 38 , wherein the microorganism is selected from the group consisting of Schizochytrium, Thraustochytrium , and Crypthecodinium.
40 . An isolated microbial strain deposited in the Culture Collection of Algae and Protozoa under accession number CCAP 3067/1, or a strain derived therefrom.
41 . A microbial oil produced by the microbial strain of claim 40 .
42 . An isolated biomass comprising microorganisms of claim 40 .
43 . A method of preparing a composition comprising combining nutritional components and LC-PUFAs, wherein the LC-PUFAs comprise DPA(n-3) and DHA, and wherein the ratio of DPA(n-3):DHA is from about 0.2:1 to about 1:1.
44 . A method of preparing a composition, comprising combining nutritional components and an oil blend, wherein the oil blend comprises a first oil comprising one or more of a plant, fish, or microbial oil, and a second oil, wherein the oil is a microbial oil having a DHA:DPA(n-3):EPA ratio of 0.01-0.7:1:0.01-0.7