SUGAR MIXTURES AND METHODS FOR PRODUCTION AND USE THEREOF
A sugar mixture comprising: monosaccharides; oligosaccharides in a ratio ≥0.06 to total saccharides; disaccharides in a ratio to total saccharides ≥0.05; pentose in a ratio to total saccharides ≥0.05; at least one alpha-bonded di-glucose; and at least one beta-bonded di-glucose. Also disclosed are methods to make and/or use such mixtures.
1 . A sugar mixture comprising:
(i) monosaccharides;
(ii) oligosaccharides in a ratio to total saccharides ≥0.06;
(iii) disaccharides in a ratio to total saccharides ≥0.05;
(iv) pentose in a ratio to total saccharides ≥0.05;
(v) at least one alpha-bonded di-glucose; and
(vi) at least one beta-bonded di-glucose.
2 . A mixture according to claim 1 , having higher oligosaccharides in a ratio to total saccharides <0.2.
3 . A mixture according to claim 1 , wherein a ratio of at least one of said alpha-bonded di-glucose and said beta-bonded di-glucose relative to total saccharides is ≥0.01.
4 . A mixture according to claim 3 , wherein a ratio of at least one of said alpha-bonded di-glucose and said beta-bonded di-glucose relative to total saccharides is ≥0.03.
5 . A mixture according to claim 1 , wherein said alpha-bonded di-glucose includes at least one member of the group consisting of maltose, isomaltose and trehalose.
6 . A mixture according to claim 1 , wherein said beta-bonded di-glucose includes at least one member selected from the group consisting of gentiobiose, sophorose and cellobiose.
7 . A method comprising:
(a) hydrolyzing a lignocellulosic material in a medium containing HCl in a ratio to (HCl+water)≥0.37 to form a hydrolyzate comprising total saccharides in a ratio to (total saccharides+water)≥0.20 by weight;
(b) de-acidifying said hydrolyzate to form a de-acidified hydrolyzate comprising: (i) total saccharides in a ratio to (total saccharides+water)≥0.35 and; (ii) total disaccharides in a ratio to total saccharides ≥0.05; and
(c) adjusting a composition of said de-acidified hydrolyzate to form a sugar mixture comprising:
(i) monosaccharides
(ii) oligosaccharides in a ratio to total saccharides >0.06:
(iii) disaccharides in a ratio to total saccharides >0.05;
(iv) pentose in a ratio to total saccharides >0.05;
(v) at least one alpha-bonded di-glucose; and
(vi) at least one beta-bonded di-glucose.
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18 . A method comprising:
(a) providing a fermentor; and
(b) fermenting a medium comprising the sugar mixture according to claim 1 in said fermentor to produce a fermentation product.
19 . A method for producing a fermentation product, the method comprising:
(a) providing a fermentor; and
(b) fermenting a medium comprising a de-acidified hydrolyzate according to claim 7 or a de-acidified preparation,
the de-acidified preparation prepared by deacidifying a preparation, the preparation comprising:
HCL; and
a sugar mixture comprising:
(i) monosaccharides;
(ii) oligosaccharides in a ratio to total saccharides >0.06;
(iii) disaccharides in a ratio to total saccharides >0.05;
(iv) pentose in a ratio to total saccharides >0.05;
(v) at least one alpha-bonded di-glucose; and
(vi) at least one beta-bonded di-glucose;
to produce the fermentation product.
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40 . A method comprising:
(a) de-acidifying an acid hydrolyzate comprising total saccharides in a ratio to (total saccharides+water)≥0.20 by weight to produce a sugar mixture with total saccharides in a ratio to (total saccharides+water) ratio ≥0.35; said mixture comprising monosaccharides, said mixture having disaccharides in a ratio to total saccharides ≥0.05; and
(b) enzymatically hydrolyzing said mixture with an enzyme capable of catalyzing hydrolysis of alpha bonds in said mixture so that at least 10 % of said disaccharides are converted to monosaccharides; and
(c) converting at least a portion of said saccharides to a conversion product.
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47 . A method according to claim 40 , wherein said enzymatically hydrolyzing comprises use of an enzyme capable of catalyzing hydrolysis of beta bonds.
48 . A method according to claim 40 , wherein enzyme includes at least one enzyme selected from the group consisting of amylases, cellulases, hemicellulases, transglucosidases, glucoamylases, alpha-glucosidases and pullulanases.
49 . A method according to claim 40 , wherein said enzymatically hydrolyzing comprises use of an immobilized enzyme.
50 . The method according to claim 40 , wherein at least a portion of said converting is conducted simultaneously with said enzymatically hydrolyzing.
51 . A method according to claim 40 , wherein the total saccharides ratio to (total saccharides+water) is ≥0.15 during said enzymatically hydrolyzing.
52 . A method according to claim 40 , wherein said enzymatically hydrolyzing comprises incubation of said mixture with a microorganism.
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