AUTOMATED POINT-OF-CARE DEVICES FOR COMPLEX SAMPLE PROCESSING AND METHODS OF USE THEREOF
The present invention provides methods and devices for simple, low power, automated processing of biological samples through multiple sample preparation and assay steps. The methods and devices described facilitate the point-of-care implementation of complex diagnostic assays in equipment-free, non-laboratory settings.
1 - 21 . (canceled)
22 . A microfluidic device comprising:
a reagent-dispensing unit comprising at least one reagent pouch comprising one or more reagents and a sealing layer;
at least one actuator element comprising spatially oriented magnets;
a microfluidic cartridge comprising a plurality of wells;
wherein said microfluidic cartridge is configured to receive magnetic particles;
wherein said microfluidic cartridge is configured for a) incremental forwards and backwards rotation about a central axis and in a predetermined sequence corresponding to steps in an assay process sequence, or b) continuous rotation for less than or equal to 360 degrees about a central axis;
wherein said microfluidic cartridge and said at least one actuator element are positioned proximate and substantially parallel to each other, and wherein rotation of said microfluidic cartridge causes the spatially oriented magnets to transport said magnetic particles between said wells; and
at least one plunger configured to contact said at least one reagent pouch and apply an actuation force to said at least one reagent pouch to rupture said sealing layer and dispense said one or more reagents to said plurality of wells.
23 . The microfluidic device of claim 22 , wherein said plurality of wells comprises at least one amplification well coupled to an amplification well pouch comprising a sealing layer and a sharp object or protrusion configured to rupture said sealing layer and dispense an amplified product contained within said amplification well when an actuation force is applied to the amplification well pouch.
24 . The microfluidic device of claim 23 , wherein the at least one actuator element comprises a plurality of spatially oriented mechanical elements configured to apply said actuation force to said amplification well pouch at a predefined time in an assay sequence and actuate the at least one sharp object or protrusion to rupture the sealing layer.
25 . The microfluidic device of claim 24 , wherein said microfluidic cartridge further comprises an integrated lateral flow strip separated from said amplification well by said amplification well pouch sealing layer, wherein rupture of said amplification well pouch allows said amplification product to flow to said lateral flow strip for detection.
26 . The microfluidic device of claim 25 , wherein said at least one actuator element comprises one or more spatially oriented heater elements configured to provide stable single temperature heat or thermal cycling for isothermal or polymerase chain reaction (PCR) based amplification of nucleic acids.
27 . The microfluidic device of claim 26 , wherein said at least one actuator element comprises a first actuator element comprising said spatially oriented magnets and a second actuator element comprising said one or more spatially oriented heater elements, and wherein said microfluidic cartridge is sandwiched between said first actuator element and said second actuator element.
28 . The microfluidic device of claim 22 , wherein said continuous rotation is powered by a wind-up spring.
29 . The microfluidic device of claim 22 further comprising:
at least one inlet conduit;
at least one reagent well;
at least one waste well;
wherein the inlet conduit, the reagent well, and the waste well are fluidically connected and configured such that there is an interface between the sealing layer of said at least one reagent pouch and the inlet conduit such that one or more reagents are delivered into the reagent well via the inlet conduit when an actuation force is applied to the at least one reagent pouch of the reagent dispensing unit and any excess reagent that overflows out of the reagent well is collected in the waste well.
30 . The microfluidic device of claim 22 further comprising a locking mechanism configured to lock the at least one plunger in a depressed position, thereby preventing backflow of said one or more reagents into the at least one reagent pouch.
31 . The microfluidic device of claim 30 , wherein the locking mechanism comprises barbed pins inside a locking bore configured to restrict the motion of the at least one plunger to a direction that facilitates the depressing of the at least one reagent pouch during the application of actuation force.
32 . The microfluidic device of claim 22 , wherein said microfluidic cartridge comprises a primary channel connecting said plurality of reagent wells.
33 . The microfluidic device of claim 22 comprising a plurality of plungers and a plurality of reagent pouches and wherein said plurality of plungers are configured to simultaneously apply said actuation force to each of the plurality of reagent pouches so as to dispense the one or more reagents from the plurality of reagent pouches simultaneously.
34 . The microfluidic device of claim 22 comprising a plurality of plungers and a plurality of reagent pouches, wherein said plurality of plungers comprise spatially oriented protrusions with varying depths so as to make contact with a desired reagent pouch from among the plurality of reagent pouches in a predetermined sequence so as to sequentially dispense the one or more reagents into the microfluidic cartridge.
35 . The microfluidic device of claim 22 further comprising a sample inlet port through which a sample may be injected introduced into the microfluidic device.
36 . The microfluidic device of claim 22 wherein said microfluidic cartridge comprises one or more lyophilized or gel reagents.
37 . A microfluidic device comprising:
a microfluidic cartridge comprising a reaction chamber;
a reagent-dispensing unit comprising at least one flow through reagent pouch, wherein said at least one flow through reagent pouch comprises a reagent, a sealing layer and a rupture element configured to rupture said sealing layer; and
wherein said flow through reagent pouch is fluidically connected to a transfer reagent source wherein, upon actuation, a transfer reagent flows into said flow through reagent pouch.
38 . The microfluidic cartridge of claim 37 , wherein said transfer reagent comprises an immiscible fluid that flows into said flow through reagent pouch upon actuation of said transfer reagent source and displaces said reagent in said flow through reagent pouch such that said reagent flows into said reaction chamber.
39 . A microfluidic device comprising:
a microfluidic cartridge comprising an inlet conduit, at least one reagent well, and a waste well;
a reagent-dispensing unit comprising a plurality of reagent pouches;
wherein said plurality of reagent pouches comprises at least a first dual reagent pouch comprising a single reagent compartment configured to contain an aqueous reagent and a non-aqueous immiscible reagent packaged together in a single reagent compartment.
40 . The microfluidic device of claim 39 , wherein said first dual reagent pouch comprises a sealing layer and wherein the aqueous reagent contained in said single reagent compartment is closer to the interface between the sealing layer and the inlet conduit than the non-aqueous immiscible reagent contained in said single reagent compartment.
41 . The microfluidic device of claim 40 , wherein said first dual reagent pouch is configured such that said aqueous reagent is dispensed to the reagent well via inlet conduit followed by said non-aqueous immiscible reagent and wherein any excess non-aqueous immiscible reagent is collected in the waste well.
42 . The microfluidic device of claim 41 , wherein the non-aqueous immiscible reagent is less dense than the aqueous reagent thereby forming an aqueous reagent layer and a non-aqueous immiscible reagent layer and wherein said aqueous reagent layer is above the non-aqueous immiscible reagent.
43 . The microfluidic device of claim 22 comprising a single motor configured to power said microfluidic device and complete an assay process sequence.