CULTURE MEDIUM COMPRISING DIPEPTIDES
The present invention relates to a culture medium, preferably a cell culture medium, comprising at least one dipeptide, the dipeptide comprising asparagine (Asn) and glutamine (Gln). The invention further relates to the use of a culture medium of the invention for culturing cells, preferably plant cells, animal cells or mammalian cells. Another aspect of the invention relates to a method of manufacturing a cell culture product comprising the steps of (i) providing a cell capable of producing said cell culture product; (ii) contacting said cell with a culture medium according to the invention; and (iii) obtaining said cell culture product from said culture medium or from said cell.
1 . A culture medium, comprising
at least one dipeptide,
said at least one dipeptide comprising asparagine (Asn) and glutamine (Gln).
2 . The culture medium of claim 1 , wherein said at least one dipeptide is Asn-Gln or Gln-Asn.
3 . The culture medium of claim 1 , wherein said at least one dipeptide is present in said culture medium at a concentration of from 0.1 to 10 mM.
4 . The culture medium of claim 1 , further comprising
at least one carbohydrate, at least one free amino acid, at least one inorganic salt, a buffering agent and/or at least one vitamin.
5 . The culture medium of claim 1 , which does not contain a growth factor.
6 . The culture medium of claim 1 , which is in a liquid form or in form of a gel, a powder, a granulate, a pellet or a tablet.
7 . The culture medium of claim 1 , which is a serum-free medium or a defined medium.
8 . The culture medium of claim 1 , which is in 2-fold, 3-fold, 3.33-fold, 4-fold, 5-fold or 10-fold concentrated form, relative to a concentration of said medium in use.
9 . A method for culturing cells, the method comprising culturing the cells using the culture medium of claim 1 .
10 . The method of claim 9 , wherein said cells are animal cells or plant cells.
11 . The method of claim 10 , wherein said cells are selected from the group consisting of CHO cells, COS cells, VERO cells, BHK cells, HEK cells, HELA cells, AE-1 cells, insect cells, fibroblast cells, muscle cells, nerve cells, stem cells, skin cells, endothelial cells and hybridoma cells.
12 . A method for culturing cells, the method comprising
culturing the cells using a dipeptide comprising asparagine (Asn) and glutamine (Gln).
13 . A method of manufacturing a cell culture product, the method comprising:
providing a cell capable of producing said cell culture product;
contacting said cell with the culture medium of claim 1 ; and
obtaining said cell culture product from said culture medium or from said cell.
14 . The method of claim 13 , wherein said cell culture product is selected from the group consisting of therapeutic protein, diagnostic protein, polysaccharide, antibody, monoclonal antibody, growth factor, interleukin, virus, virus-like particle and enzyme.