METHOD TO PRODUCE SEEDS RAPIDLY THROUGH ASEXUAL PROPAGATION OF CUTTINGS IN LEGUMES
The present invention is directed at a method to produce a substantial number of seeds (e.g., 10,000) from a single plant. The method involves growing a mother plant under conditions designed to substantially maintain the mother plant in a vegetative state and substantially delay reaching reproductive stage. Then, removing the apical meristem once the mother plant has reached a desired stage of growth. The method further involves removing one or more branches from the mother plant (as branch cuttings). The method additionally involves maintaining each of the branch cuttings in growth media under acclimation conditions until each of the branch cuttings resume growing vegetatively as clone plants, then inducing the clone plants to flower and once the clone plants have been induced to flower, returning them to vegetative growth conditions. The clone plants are then maintained under vegetative growth conditions until they have produced a desired number of seeds.
1 . A method to produce seeds from a single mother plant having an apical meristem, wherein the single mother plant is in one or both of a vegetative state and reproductive state, the vegetative state having vegetative stages and the reproductive state having reproductive stages, the method comprising:
(a) growing the mother plant under conditions designed to substantially maintain the mother plant in a vegetative state and substantially delay reaching reproductive stage;
(b) removing the apical meristem from the mother plant once the mother plant has reached a desired stage of growth;
(c) removing one or more branches from the mother plant as one or more branch cuttings wherein the one or more branches were sufficiently developed on the mother plant following the removal of the apical meristem;
(d) maintaining each of the one or more branch cuttings in growth media under acclimation conditions until each of the branch cuttings resume growing vegetatively as clone plants;
(e) inducing the clone plants to flower;
(f) once the clone plants have been induced to flower, returning the clone plants to vegetative growth conditions; and
(g) maintaining the clone plants under vegetative growth conditions until they have produced a desired number of seeds.
2 . The method of claim 1 wherein the mother plant is a short-day plant, the conditions to substantially maintain the mother plant in a vegetative state and substantially delay reaching reproductive stage comprising applying long-day conditions to the mother plant.
3 . The method of claim 1 wherein the desired level of growth is determined by assessing at least one of the vegetative and reproductive stages of the mother plant to determine whether the mother plant has progressed enough to remove the apical meristem.
4 . The method of claim 1 further comprising exposing each of the one or more branch cuttings to an auxin solution before maintaining the branch cuttings in the growth media.
5 . The method of claim 4 wherein exposing is selected from the group comprising applying the auxin solution to the branch cutting by brushing on, dripping on, pouring on, or spraying on and placing the branch cutting in a container with the auxin solution.
6 . The method of claim 5 wherein the auxin solution includes auxin, nutrients, and pH balanced water solution
7 . The method of claim 1 wherein maintaining the branch cuttings in growth media under acclimation conditions until the branch cutting resumes growing vegetatively further comprises exposing the branch cuttings to high relative humidity and vegetative growing conditions.
8 . The method of claim 1 further comprises exposing the growth media to an auxin solution prior to receiving the branch cuttings therein.
9 . The method of claim 1 further comprises maintaining the apical meristem cutting in a growth media under acclimation conditions until the apical meristem cutting begins growing vegetatively.
10 . The method of claim 9 further comprises exposing the apical meristem cutting to an auxin solution.
11 . The method of claim 10 wherein maintaining the apical meristem cutting in growth media under acclimation conditions until the apical meristem cutting resumes growing vegetatively further comprises exposing the apical meristem cutting to high relative humidity and vegetative growing conditions.