MICROPLATE CARRIER
Disclosed herein, inter alia, are devices, microplates, compositions, kits, and methods for interrogating biological samples.
1 . An imaging system, comprising:
an image sensor configured to detect light emissions from a microplate assembly, wherein said microplate assembly comprises:
a microplate receiver frame defining a pocket;
at least one microplate section; and
a planar support positioned on a bottom of the at least one microplate section, and wherein the at least one microplate section comprises a maximum coefficient of linear thermal expansion of about 2.0×10 −4 /K to about 1.0×10 −5 /K;
wherein the pocket of the microplate receiver frame is sized and shaped to receive a one or more microplate sections.
2 . The imaging system of claim 1 , wherein the microplate section comprises a maximum coefficient of linear thermal expansion of about 1.0×10 −5 /K, about 1.5×10 −5 /K, about 2.0×10 −5 /K, about 2.5×10 −5 /K, about 3.0×10 −5 /K, about 3.5×10 −5 /K, about 4.0×10 −5 /K, about 4.5×10 −5 /K, about 5.0×10 −5 /K, about 5.5×10 −5 /K, about 6.0×10 −5 /K, about 6.5×10 −5 /K, about 7.0×10 −5 /K, about 7.5×10 −5 /K, about 8.0×10 −5 /K, about 8.5×10 −5 /K, about 9.0×10 −5 /K, about 9.5×10 −5 /K.
3 . (canceled)
4 . (canceled)
5 . The imaging system of claim 1 , wherein the planar support comprises a maximum coefficient of linear thermal expansion less than the coefficient of linear thermal expansion of said microplate section.
6 . (canceled)
7 . The imaging system of claim 1 , wherein a bottom region of the microplate section is glass.
8 . The imaging system of claim 7 , wherein said glass is functionalized glass, comprising one or more topographical modifications, bioconjugate reactive moieties, or biomolecules.
9 . (canceled)
10 . The imaging system of claim 1 , wherein the pocket of the frame is sized and shaped to receive a plurality of the microplate sections arranged in a side-by-side configuration.
11 . The imaging system of claim 1 , wherein the microplate section includes a first retention element and the microplate receiver includes a second retention element, and wherein the first retention element mechanically interacts with the second retention element to retain the microplate section within the pocket.
12 .- 16 . (canceled)
17 . The imaging system of claim 1 , comprising 1, 2, 3, or 4 microplate sections.
18 . The imaging system of claim 1 , comprising 2 microplate sections.
19 . The imaging system of claim 18 , wherein each microplate section comprises 6, 8, 10, 12, 16, 18, 24, 30, 36, 40, 42, 46, 48, 96, 144, or 192 wells.
20 .- 22 . (canceled)
23 . The imaging system of claim 1 , wherein the planar support is glass and comprises a cell or tissue section.
24 . (canceled)
25 . The imaging system of claim 23 , wherein said planar support is attached via an adhesive to said microplate section.
26 . (canceled)
27 . (canceled)
28 . (canceled)
29 . A microplate section, comprising
a planar support attached to a solid support comprising a plurality of wells;
wherein solid support comprises a maximum coefficient of linear thermal expansion of about 2.0×10 −4 /K to about 1.0×10 −5 /K; and
and the planar support comprises a maximum coefficient of linear thermal expansion of about 0.5×10 −5 /K to about 8.0×10 −7 /K.
30 . (canceled)
31 . The microplate section of claim 29 , comprising one or more bores, slots, seats, retention mechanisms, clips, or other structures sized and shaped to receive, align, and secure a microplate section within a microplate receiver.
32 . (canceled)
33 . (canceled)
34 . The microplate section of claim 29 , wherein the planar support comprises a cell-permissive coating capable of adhering live cells.
35 . (canceled)
36 .- 37 . (canceled)
38 . The microplate section of claim 29 , wherein the planar support comprises a hydrogel.
39 . The microplate section of claim 38 , wherein said hydrogel comprises polymerized monomers of acrylamide, bis-acrylamide, polyacrylamide and derivatives thereof, poly(ethylene glycol) and derivatives thereof (e.g., PEG-acrylate (PEG-DA), PEG-RGD), gelatin-methacryloyl (GelMA), methacrylated hyaluronic acid (MeHA), polyaliphatic polyurethanes, polyether polyurethanes, polyester polyurethanes, polyethylene copolymers, polyamides, polyvinyl alcohols, polypropylene glycol, polytetramethylene oxide, polyvinyl pyrrolidone, polyacrylamide, poly(hydroxyethyl acrylate), and poly(hydroxyethyl methacrylate), collagen, hyaluronic acid, chitosan, dextran, agarose, gelatin, alginate, protein polymers, methylcellulose, or combinations thereof.
40 .- 44 . (canceled)
45 . A method of imaging a cell, said method comprising:
contacting a microplate section and immobilizing the with the cell to a planar support at the bottom of the microplate section; and obtaining an image of the cell with the imaging system of claim 1 , thereby imaging the cell.
46 . A method of detecting a biomolecule in a tissue section, the method comprising: immobilizing the tissue section onto a microplate section, optionally permeabilizing the immobilized tissue section; and contacting the biomolecule in the tissue section with a detection agent, and detecting the detection agent with the imaging system of claim 1 , thereby detecting the biomolecule in the tissue section.
47 .- 51 . (canceled)