SELF ASSEMBLY OF BEADS ON SUBSTRATES
Provided herein are methods, systems, and kits for generating a self-assembled monolayer of beads adjacent to (e.g., on or across) a substrate. The methods, systems, and kits provided herein may comprise contacting a substrate with a plurality of beads, which may comprise nucleic acid molecules coupled thereto, and providing conditions sufficient for the plurality of beads to self-assemble adjacent to the substrate. Sequencing of the nucleic acid molecules may be performed.
1 .- 51 . (canceled)
52 . A method for self-assembly of a plurality of beads, comprising:
(a) providing a substrate and said plurality of beads, wherein said substrate is unpatterned and substantially planar;
(b) dispensing said plurality of beads adjacent to said substrate; and
(c) subjecting said substrate or said plurality of beads to conditions sufficient for self-assembly of said plurality of beads adjacent to said substrate.
53 . The method of claim 52 , wherein said substrate is a solid or semi-solid substrate, and wherein said plurality of beads is a plurality of solid or semi-solid beads.
54 . The method of claim 52 , wherein said plurality of beads comprises a plurality of nucleic acid molecules coupled thereto.
55 . The method of claim 54 , further comprising, sequencing said plurality of nucleic acid molecules.
56 . The method of claim 55 , wherein said sequencing comprises flow sequencing, which flow sequencing comprises (i) providing a reagent comprising a first plurality of nucleotides to said plurality of beads or said substrate, (ii) detecting a nucleotide from said first plurality of nucleotides, (iii) providing an additional reagent comprising a second plurality of nucleotides to said plurality of beads or said substrate, and (iv) detecting an additional nucleotide from said second plurality of nucleotides.
57 . The method of claim 52 , wherein beads of said plurality of beads are from about 0.1 microns to about 10 microns in diameter.
58 . The method of claim 52 , further comprising, prior to (b), wetting said substrate.
59 . The method of claim 58 , wherein said wetting comprises wetting said substrate with an ionic buffer.
60 . The method of claim 59 , wherein said ionic buffer comprises magnesium.
61 . The method of claim 58 , wherein said wetting renders said substrate hydrophilic.
62 . The method of claim 52 , wherein said substrate comprises a silicon wafer.
63 . The method of claim 52 , wherein said substrate comprises a silicon oxide layer.
64 . The method of claim 52 , wherein said substrate comprises a glass wafer.
65 . The method of claim 52 , wherein (a) comprises providing said plurality of beads in a solution, and wherein (b) comprises contacting said substrate with said solution.
66 . The method of claim 65 , wherein (b) comprises providing said solution in one or more droplets to said substrate and allowing said solution to spread adjacent to said substrate.
67 . The method of claim 65 , further comprising, rotating said substrate to disperse said plurality of beads across said substrate.
68 . The method of claim 67 , wherein said rotating is performed from about 1 revolution per minute (rpm) to about 8000 rpm.
69 . The method of claim 52 , wherein, subsequent to (c), said plurality of beads is arranged in a self-assembled monolayer.
70 . The method of claim 69 , wherein said self-assembled monolayer has a bead-to-bead tolerance of approximately twice a diameter of a bead of said plurality of beads.
71 . The method of claim 69 , wherein said self-assembled monolayer is arranged such that individual beads of said plurality of beads are distinguishable by microscopy.