Devices useful for sample manipulation
Disclosed herein, inter alia, are devices and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections.
1. A tissue catch tray assembly, comprising:
a tissue catch tray formed of a frame that forms a periphery of a cavity having an upper end and unenclosed lower end, wherein the frame has an outer peripheral wall and an inner peripheral wall concentrically positioned within the outer peripheral wall wherein the outer peripheral wall and the inner peripheral wall collectively form therebetween a peripheral trough that surrounds the cavity, and wherein the cavity is configured to contain a three-dimensional polymer slab;
a handle extending outwardly from the tissue catch tray; and
a cover film removably positionable on a bottom of the frame such that the cover film encloses a lower end of the cavity and forms a bottom surface of the cavity when positioned on the bottom of the frame.
2. The tissue catch tray assembly of claim 1 , wherein the tissue catch tray comprises a rib on the frame, wherein the rib extends into the cavity.
3. The tissue catch tray assembly of claim 2 , wherein the rib extends into the cavity from a peripheral wall of the frame, and wherein the rib extends around an inner perimeter of the cavity.
4. The tissue catch tray assembly of claim 3 , wherein the rib extends around an entire inner perimeter of the cavity.
5. The tissue catch tray assembly of claim 3 , wherein the rib extends around only a portion of the inner perimeter of the cavity.
6. The tissue catch tray assembly of claim 1 , further comprising a planar substrate removably positionable on a top of the frame such that the substrate encloses a top end of the cavity such that the cavity is positioned between the planar substrate and the cover film.
7. The tissue catch tray assembly of claim 6 , further comprising a lid that removably attachable to a top end of the frame such that the substrate is interposed between the lid and the cavity.
8. The tissue catch tray assembly of claim 1 , wherein the frame forms an outer peripheral wall that surrounds the cavity and an inner peripheral wall that surrounds the cavity, and wherein the peripheral trough is positioned between the outer peripheral wall and the inner peripheral wall.
9. The tissue catch tray assembly of claim 1 , wherein the cavity forms a mold for forming a three-dimensional polymer slab.
10. The tissue catch tray assembly of claim 1 , wherein the handle comprises a planar structure that extends outwardly from a side of the outer peripheral wall of the tissue catch tray.
11. A method of immobilizing a tissue section, the method comprising:
contacting a vessel comprising a tissue section with a tissue catch tray having an outer peripheral wall and an inner peripheral wall concentrically positioned within the outer peripheral wall wherein the outer peripheral wall and the inner peripheral wall collectively form therebetween a trough, wherein the vessel comprises water and wherein the tissue section is at or on the surface of the water, and wherein the tissue catch tray comprises:
a frame comprising a three-dimensional polymer slab;
a handle extending outwardly from the tissue catch tray; and
removing the tissue catch tray from the vessel using the handle, wherein the tissue section is immobilized to the tissue catch tray.