ALGICIDAL ORGANISMS
Provided are compositions derived from Beauveria bassiana, Brevibacillus brevis, Streptomyces spororaveus, Paenibacillus elgii , or Streptomyces sp. fermentation, having algicidal properties, and method of use thereof.
1 . A method for inhibiting the growth of an algae comprising the steps of:
forming an algicidal composition by:
shake flask fermenting, for at least 72 hours, Beauveria bassiana 0379 (NRRL-67406) in a broth containing one or more of: nitrogen, phosphate, carbon, and inorganic salts;
collecting a whole cell broth from the shake flask fermentation, the whole cell broth comprising Beauveria bassiana 0379 (NRRL-67406) cells having a final concentration of at least 42 ppm;
combining the whole cell broth with a carrier, diluent, adjuvant and/or surfactant; and
introducing the algicidal composition in a location where inhibition is desired, in amounts effective for inhibiting said algae.
2 . The method of claim 1 , wherein said location is a liquid comprising a body of water or paint.
3 . The method of claim 1 , wherein said location is a solid surface selected from the group consisting of plastic, concrete, wood, fiberglass, pipes made of iron and polyvinyl chloride, and surfaces covered with coating materials and/or paint.
4 . The method of claim 1 , wherein said algae comprises Chlamydomonas reinhardtii, Pesudokirchneriella subacpitata, Scenedesmus quadricata, Anabaena sp., Oscillatoria temuis, Microcystis aeruginosa, Navicula sp., Spirogyra sp., or Chara sp.
5 . The method of claim 1 , wherein the algicidal composition is in one or more of: a dustable powder, a soluble powder, water soluble granules, water dispersible granules, wettable powders, granules, soluble concentrates, oil miscible liquids, ultra-low volume liquids, emulsifiable concentrates, dispersible concentrates, emulsions, micro-emulsions, suspension concentrates, oil-based suspension concentrate, aerosols, fogging/smoke, capsule suspensions or seed treatment formulation.
6 . The method of claim 1 , wherein the algicidal composition further comprises a chemical and/or biological algaecide.
7 . The method of claim 6 , wherein the biological algaecide comprises Burkholderia sp.
8 . The method of claim 6 , wherein the chemical algaecide comprises 3-(4-chloro-2,6-dimethylphenyl)-4-hydroxy-8-oxa-1-azaspiro[4,5]dec-3-en-2-one; 3-(4′-chloro-2,4-dimethyl[1,1′-biphenyl]-3-yl)-4-hydroxy-8-oxa-1-azaspiro-[4,5]dec-3-en-2-one; 4-[[(6-chloro-3-pyridinyl)methyl]methylamino]-2 (5H)-furanone; 4-[[(6-chloro-3-pyridinyl)methyl]cyclopropylamino]-2 (5H)-furanone; 3-chloro-N2-[(1S)-1-methyl-2-(methylsulfonyl)ethyl]-N1-[2-methyl-4-[1,2,2,2-tetrafluoro-1-(trifluoromethyl)ethyl]phenyl]-1,2-benzenedicarboxamide; 2-cyano-N-ethyl-4-fluoro-3-methoxy-benenesulfonamide; 2-cyano-N-ethyl-3-methoxy-benzenesulfonamide; 2-cyano-3-difluoromethoxy-N-ethyl-4-fluoro-benzenesulfonamide; 2-cyano-3-fluoromethoxy-N-ethyl-benzenesulfonamide; 2-cyano-6-fluoro-3-methoxy-N,N-dimethyl-benzenesulfonamide; 2-cyano-N-ethyl-6-fluoro-3-methoxy-N-methyl-benzenesulfonamide; 2-cyano-3-difluoromethoxy-N,N-dimethylbenzenesulfon-amide; 3-(difluoromethyl)-N-[2-(3,3-dimethylbutyl)phenyl]-1-methyl-1H-pyrazole-4-carboxamide; N-ethyl-2,2-dimethylpropionamide-2-(2,6-dichloro-α,α,α,-trifluoro-p-tolyl) hydrazone; N-ethyl-2,2-dichloro-1-methylcyclopropane-carboxamide-2-(2,6-dichloro-α,α,α,-trifluoro-p-tolyl) hydrazone nicotine; O-{(E-)-[2-(4-chloro-phenyl)-2-cyano-1-(2-trifluoromethylphenyl)-vinyl]}S-methyl thiocarbonate; (E)-N1-[(2-chloro-1,3-thiazol-5-ylmethyl)]-N2-cyano-N1-methylacetamidine; 1-(6-chloropyridin-3-ylmethyl)-7-methyl-8-nitro-1,2,3,5,6,7-hexahydro-imidazo[1,2-a]pyridin-5-ol; 4-[4-chlorophenyl-(2-butylidine-hydrazono)methyl)]phenyl mesylate; or N-Ethyl-2,2-dichloro-1-methylcyclopropanecarboxamide-2-(2,6-dichloro-α,α,α,-trifluoro-p-tolyl) hydrazone.
9 . A method for inhibiting the growth of an algae comprising the steps of:
forming an algicidal composition by:
shake flask fermenting Beauveria bassiana 0379 (NRRL-67406) in a broth containing one or more of: nitrogen, phosphate, carbon, and inorganic salts;
collecting a whole cell broth from the shake flask fermentation, the whole cell broth comprising Beauveria bassiana 0379 (NRRL-67406) cells having a final concentration of at least 42 ppm;
combining the whole cell broth with a carrier, diluent, adjuvant and/or surfactant, and a chemical algaecide, wherein said chemical algaecide comprises at least one of: 3-(4-chloro-2,6-dimethylphenyl)-4-hydroxy-8-oxa-1-azaspiro[4,5]dec-3-en-2-one; 3-(4′-chloro-2,4-dimethyl[1,1′-biphenyl]-3-yl)-4-hydroxy-8-oxa-1-azaspiro-[4,5]dec-3-en-2-one; 4-[[(6-chloro-3-pyridinyl)methyl]methylamino]-2 (5H)-furanone; 4-[[(6-chloro-3-pyridinyl)methyl]cyclopropylamino]-2 (5H)-furanone; 3-chloro-N2-[(1S)-1-methyl-2-(methylsulfonyl)ethyl]-N1-[2-methyl-4-[1,2,2,2-tetrafluoro-1-(trifluoromethyl)ethyl]phenyl]-1,2-benzenedicarboxamide; 2-cyano-N-ethyl-4-fluoro-3-methoxy-benenesulfonamide; 2-cyano-N-ethyl-3-methoxy-benzenesulfonamide; 2-cyano-3-difluoromethoxy-N-ethyl-4-fluoro-benzenesulfonamide; 2-cyano-3-fluoromethoxy-N-ethyl-benzenesulfonamide; 2-cyano-6-fluoro-3-methoxy-N,N-dimethyl-benzenesulfonamide; 2-cyano-N-ethyl-6-fluoro-3-methoxy-N-methyl-benzenesulfonamide; 2-cyano-3-difluoromethoxy-N,N-dimethylbenzenesulfon-amide; 3-(difluoromethyl)-N-[2-(3,3-dimethylbutyl)phenyl]-1-methyl-1H-pyrazole-4-carboxamide; N-ethyl-2,2-dimethylpropionamide-2-(2,6-dichloro-α,α,α,-trifluoro-p-tolyl) hydrazone; N-ethyl-2,2-dichloro-1-methylcyclopropane-carboxamide-2-(2,6-dichloro-α,α,α,-trifluoro-p-tolyl) hydrazone nicotine; O-{(E-)-[2-(4-chloro-phenyl)-2-cyano-1-(2-trifluoromethylphenyl)-vinyl]}S-methyl thiocarbonate; (E)-N1-[(2-chloro-1,3-thiazol-5-ylmethyl)]-N2-cyano-N1-methylacetamidine; 1-(6-chloropyridin-3-ylmethyl)-7-methyl-8-nitro-1,2,3,5,6,7-hexahydro-imidazo[1,2-a]pyridin-5-ol; 4-[4-chlorophenyl-(2-butylidine-hydrazono)methyl)]phenyl mesylate; or N-Ethyl-2,2-dichloro-1-methylcyclopropanecarboxamide-2-(2,6-dichloro-α,α,α,-trifluoro-p-tolyl) hydrazone; and
introducing the algicidal composition in a location where inhibition is desired, in amounts effective for inhibiting said algae.
10 . The method of claim 9 , wherein the location is a liquid comprising a body of water or paint.
11 . The method of claim 9 , wherein said location is a solid surface selected from the group consisting of plastic, concrete, wood, fiberglass, pipes made of iron and polyvinyl chloride, and surfaces covered with coating materials and/or paint.
12 . The method of claim 9 , wherein said algae comprises Chlamydomonas reinhardtii, Pesudokirchneriella subacpitata, Scenedesmus quadricata, Anabaena sp., Oscillatoria temuis, Microcystis aeruginosa, Navicula sp., Spirogyra sp., or Chara sp.
13 . A method for inhibiting the growth of an algae comprising the steps of:
forming an algicidal composition by:
shake flask fermenting Beauveria bassiana 0379 having NRRL deposit number 67406 in a broth containing one or more of nitrogen, carbon, and inorganic salts, until the shake flask fermentation has an increase in turbidity of at least two fold compared to a starting turbidity of the shake flask fermentation;
collecting a whole cell broth, filtrate, supernatant, and/or extract collected from the shake flask fermentation, the whole cell broth, filtrate, supernatant, and/or extract containing Beauveria bassiana 0379 (NRRL-67406) cells;
adding a carrier, diluent, adjuvant and/or surfactant to the whole cell broth, filtrate, supernatant, and/or extract containing Beauveria bassiana 0379 (NRRL-67406) cells; and
introducing the algicidal composition in a location where inhibition is desired, in amounts effective for inhibiting said algae.
14 . The method of claim 13 , wherein said location is a liquid comprising a body of water or paint.
15 . The method of claim 13 , wherein said location is a solid surface selected from the group consisting of plastic, concrete, wood, fiberglass, pipes made of iron and polyvinyl chloride, and surfaces covered with coating materials and/or paint.
16 . The method of claim 13 , wherein said algae comprises Chlamydomonas reinhardtii, Pesudokirchneriella subacpitata, Scenedesmus quadricata, Anabaena sp., Oscillatoria temuis, Microcystis aeruginosa, Navicula sp., Spirogyra sp., or Chara sp.
17 . The method of claim 13 , wherein the algicidal composition is in one or more of: a dustable powder, a soluble powder, water soluble granules, water dispersible granules, wettable powders, granules, soluble concentrates, oil miscible liquids, ultra-low volume liquids, emulsifiable concentrates, dispersible concentrates, emulsions, micro-emulsions, suspension concentrates, oil-based suspension concentrate, aerosols, fogging/smoke, capsule suspensions or seed treatment formulation.
18 . The method of claim 13 , wherein the algicidal composition further comprises a chemical and/or a biological algaecide.
19 . The method of claim 13 , wherein the biological algaecide comprises Burkholderia sp.
20 . The method of claim 13 , wherein the chemical algaecide comprises 3-(4-chloro-2,6-dimethylphenyl)-4-hydroxy-8-oxa-1-azaspiro[4,5]dec-3-en-2-one; 3-(4′-chloro-2,4-dimethyl[1,1′-biphenyl]-3-yl)-4-hydroxy-8-oxa-1-azaspiro-[4,5]dec-3-en-2-one; 4-[[(6-chloro-3-pyridinyl)methyl]methylamino]-2 (5H)-furanone; 4-[[(6-chloro-3-pyridinyl)methyl]cyclopropylamino]-2 (5H)-furanone; 3-chloro-N2-[(1S)-1-methyl-2-(methylsulfonyl)ethyl]-N1-[2-methyl-4-[1,2,2,2-tetrafluoro-1-(trifluoromethyl)ethyl]phenyl]-1,2-benzenedicarboxamide; 2-cyano-N-ethyl-4-fluoro-3-methoxy-benenesulfonamide; 2-cyano-N-ethyl-3-methoxy-benzenesulfonamide; 2-cyano-3-difluoromethoxy-N-ethyl-4-fluoro-benzenesulfonamide; 2-cyano-3-fluoromethoxy-N-ethyl-benzenesulfonamide; 2-cyano-6-fluoro-3-methoxy-N,N-dimethyl-benzenesulfonamide; 2-cyano-N-ethyl-6-fluoro-3-methoxy-N-methyl-benzenesulfonamide; 2-cyano-3-difluoromethoxy-N,N-dimethylbenzenesulfon-amide; 3-(difluoromethyl)-N-[2-(3,3-dimethylbutyl)phenyl]-1-methyl-1H-pyrazole-4-carboxamide; N-ethyl-2,2-dimethylpropionamide-2-(2,6-dichloro-α,α,α,-trifluoro-p-tolyl) hydrazone; N-ethyl-2,2-dichloro-1-methylcyclopropane-carboxamide-2-(2,6-dichloro-α,α,α,-trifluoro-p-tolyl) hydrazone nicotine; O-{(E-)-[2-(4-chloro-phenyl)-2-cyano-1-(2-trifluoromethylphenyl)-vinyl]}S-methyl thiocarbonate; (E)-N1-[(2-chloro-1,3-thiazol-5-ylmethyl)]-N2-cyano-N1-methylacetamidine; 1-(6-chloropyridin-3-ylmethyl)-7-methyl-8-nitro-1,2,3,5,6,7-hexahydro-imidazo[1,2-a]pyridin-5-ol; 4-[4-chlorophenyl-(2-butylidine-hydrazono)methyl)]phenyl mesylate; or N-Ethyl-2,2-dichloro-1-methylcyclopropanecarboxamide-2-(2,6-dichloro-α,α,α,-trifluoro-p-tolyl) hydrazone.