Mononuclear Cell Derived NK Cells
Cord blood or peripheral blood NK cells are prepared from whole blood mononuclear cells without the need to isolate CD34+ hematopoietic stem cells or NK cells, and without the need for a feeder layer. Advantageously, the methods presented herein use an enrichment process that uses antiCD16 agonist antibodies, antiCD3 antibodies, and N-803. Moreover, contemplated processes are suitable for adaptation into a fully automated production process (GMP in a box).
1 . A method of expanding NK cells from a mixture of mononuclear cells, the method comprising:
providing in a container a mixture of the mononuclear cells that contains equal or less than 5% NK cells;
contacting the mixture of the mononuclear cells with an anti-CD16 antibody and a stabilized Interleukin-15 (IL-15) compound to activate NK cells; and
feeding in the same container the activated NK cells with a medium containing the stabilized IL-15 compound until the activated NK cells constitute at least about 80% of all live cells or until the activated NK cells are enriched to an at least 80-fold expansion.
2 . The method of claim 1 wherein the mixture of the mononuclear cells is obtained from whole blood or cord blood.
3 . The method of claim 1 , wherein the mixture of the mononuclear cells contains equal or less than 3% NK cells.
4 . The method of claim 1 , wherein the anti-CD16 antibody is present at a concentration of between 0.05-0.5 mcg/ml.
5 . The method of claim 1 , wherein the stabilized IL-15 compound is present at a concentration of between 0.1-1.0 nM.
6 . The method of claim 1 , wherein the stabilized IL-15 compound is N-803.
7 . The method of claim 1 , wherein the stabilized IL-15 compound is a TxM type fusion protein complex.
8 . The method of claim 1 , wherein the medium containing the stabilized IL-15 compound comprises human AB serum.
9 . The method of claim 1 , wherein the step of feeding comprises sequentially feeding at an interval of about every 72 hours.
10 . The method of claim 1 , wherein the step of feeding is performed until a total cell number of about 0.5-5.0×10 9 cells is reached.
11 . The method of claim 1 , wherein the step of feeding the activated NK cells is performed until NK cells are enriched to an at least 100-fold expansion.
12 . The method of claim 1 , wherein the step of feeding the activated NK cells is performed in an automated manner.
13 . A method of expanding NK cells in an automated bioreactor, the method comprising:
incubating a mixture of mononuclear cells in an activation medium containing a stabilized IL-15 compound and an anti-CD16 antibody for a time sufficient to activate NK cells;
wherein the mixture of mononuclear cells is contained in a cell culture container while incubating the mixture;
measuring growth of the cells while the cells are in the container; automatically feeding the cells using a medium containing the stabilized IL-15 compound until the activated NK cells constitute at least about 80% of all live cells or until the activated NK cells are enriched to an at least 80-fold expansion, wherein the feeding is controlled by a predetermined schedule and/or a result from the step of measuring growth of the cells;
terminating feeding the cells wherein the terminating is controlled by a predetermined schedule and/or a result from the step of measuring growth of the cells.
14 . The method of claim 13 , wherein the container has a volume of between about 200 ml and about 2,500 ml.
15 . The method of claim 13 , wherein the step of measuring growth of the cells is performed through a wall of the container.
16 . The method of claim 13 , wherein the activation medium contains the stabilized IL-15 compound at a concentration of between 0.1-1.0 nM and the anti-CD16 antibody at a concentration of between 0.05-0.5 mcg/ml.
17 . The method of claim 13 , wherein the time sufficient to activate NK cells is between 24 hours and 96 hours.
18 . The method of claim 13 , wherein the medium containing the stabilized IL-15 compound contains the stabilized IL-15 compound at a concentration of between 0.1-1.0 nM.
19 . The method of claim 13 , wherein the cells are fed until a total cell number of about 0.5-5.0×10 9 cells is reached.
20 . The method of claim 13 , wherein the cells are fed until NK cells are enriched to an at least 100-fold expansion.