MULTIPLEXED BIOLOGICAL ASSAY DEVICE WITH ELECTRONIC READOUT
This invention relates generally to devices, systems, and methods for performing biological assays by using indicators that modify one or more optical properties of the assayed biological samples. The subject methods include generating a reaction product by carrying out a biochemical reaction on the biological sample introduced into a device and reacting the reaction product with an indicator capable of generating a detectable change in an optical property of the biological sample to indicate the presence, absence, or amount of analyte suspected to be present in the sample.
1 - 126 . (canceled)
127 . An assembly for performing a biological assay, the assembly comprising:
a first piece, a second piece, a common sample receiving inlet, and a single sensing region,
wherein the first and second pieces are operatively coupled to create a plurality of fluidic pathways comprising (i) a plurality of fluid channels extending from and in fluidic communication with the common sample receiving inlet and (ii) a plurality of fluidic chambers, each fluidic chamber comprising a fluid inlet in fluidic communication with a terminus of one of the plurality of fluid channels;
wherein the second piece comprises a plurality of light pipes; and
wherein each fluidic chamber is substantially equidistant from a single sensing region.
128 . The assembly of claim 127 , further comprising a gasket located between the first piece and the second piece, the gasket operatively coupled to the first piece and the second piece to form fluid seals between the plurality of fluidic chambers.
129 . The assembly of claim 127 , wherein each of the plurality of fluidic chambers comprises an outlet vent that is sealed by a self-sealing vent material.
130 . The assembly of claim 127 , wherein each of the plurality of fluidic chambers comprises an outlet vent that is sealed by a hydrophobic membrane.
131 . The assembly of claim 127 , further comprising a circuit board comprising a plurality of light emitting elements.
132 . A system for performing a biological assay, the system comprising:
(i) the assembly of claim 127 ; and
(ii) a circuit board operatively coupled to the fluidic chambers, the circuit board comprising:
a microprocessor,
a plurality of light emitting elements,
a photosensor optically coupled to the single sensing region,
a heating element,
a temperature sensor, and
an electronic display mechanism,
wherein the microprocessor is programmed to analyze signals received from the photosensor, to generate signals transmitted to the heating element, to analyze signals received from the temperature sensor, and to generate signals transmitted to the electronic display mechanism.
133 . The system of claim 132 , wherein the photosensor is configured to detect a color change, an absorbance change, or a fluorescence change.
134 . A system for performing a biological assay, the system comprising:
(i) an assembly, the assembly comprising a first piece and a second piece operatively coupled to create a plurality of fluidic chambers, wherein the second piece comprises a plurality of light pipes, the first plurality of light pipes capable of transmitting light between the plurality of fluidic chambers and a single sensing region; and
(ii) a circuit board operatively coupled to the fluidic chambers, the circuit board comprising:
a microprocessor,
a plurality of light emitting elements,
a photosensor optically coupled to the single sensing region,
a heating element,
a temperature sensor, and
an electronic display mechanism,
wherein the microprocessor is programmed to analyze signals received from the photosensor, to generate signals transmitted to the heating element, to analyze signals received from the temperature sensor, and to generate signals transmitted to the electronic display mechanism.
135 . A method of determining one or more characteristics of a sample based on a modified optical property of the sample, the method comprising:
providing a reaction mixture to the plurality of fluidic chambers of the system of claim 132 ,
wherein the reaction mixture comprises a biological sample, an optical property modifying reagent, and an assay reagent;
generating a reaction using the biological sample and the assay reagents, the reaction generating a product;
reacting the product with the optical property modifying reagent, wherein the reacting is capable of modifying an optical property of the optical property modifying reagent to allow detection of the modified optical property indicative of the presence of a suspected analyte in the biological sample;
causing each of the plurality of light emitting elements to emit light; and
determining one or more characteristics of the sample using the photosensor based on the modified optical property.