PROTEINS AND IMMUNIZING COMPOSITIONS CONTAINING PASTEURELLA PROTEINS AND METHODS OF USE
The present invention provides isolated proteins isolatable from a Pasteurella spp. such as P. multocida . Also provided by the present invention are compositions that include one or more of the proteins, and methods for making and methods for using the proteins.
1 - 27 . (canceled)
28 . A composition comprising:
an isolated protein having at least 80% similarity to amino acids 26-805 of SEQ ID NO: 44; and
an adjuvant.
29 . The composition of claim 1 , further comprising:
an isolated protein having at least 80% similarity to amino acids 25-968 of SEQ ID NO:2,
an isolated protein having at least 80% similarity to amino acids 27-790 of SEQ ID NO:4,
an isolated protein having at least 80% similarity to amino acids 23-727 of SEQ ID NO:6,
an isolated protein having at least 80% similarity to amino acids 25-964 of SEQ ID NO:8,
an isolated protein having at least 80% similarity to amino acids 26-848 of SEQ ID NO: 10,
an isolated protein having at least 80% similarity to amino acids 27-784 of SEQ ID NO: 12,
an isolated protein having at least 80% similarity to amino acids 25-742 of SEQ ID NO: 14, or a combination thereof.
30 . The composition of claim 28 further comprising: at least one isolated protein having a molecular weight of 249 kDa, 60 kDa, 42 kDa, 38 kDa, 27 kDa, 26 kDa, or 22 kDa, wherein the proteins are isolated from a P. multocida.
31 . The composition of claim 28 further comprising a pharmaceutically acceptable carrier.
32 . A composition comprising a bacterium engineered to express the protein of claim 28 .
33 . The composition of claim 32 , wherein the bacterium is gram negative.
34 . The composition of claim 33 , wherein the gram negative bacterium is E. coli, Salmonella spp., or Pasteurella spp.
35 . The composition of claim 34 , wherein the Pasteurella spp. is P. multocida.
36 . The composition of claim 32 , wherein the bacterium is inactivated.
37 . The composition of claim 32 , further comprising an andjuvant.
38 . A composition comprising a whole cell expressing the protein of claim 28 and an adjuvant.
39 . The composition of claim 38 , wherein the bacterium is P. multocida.
40 . The composition of claim 38 , wherein the bacterium is inactivated.
41 . A method comprising: administering to a subject an amount of the composition of claim 28 effective to induce the subject to produce antibody that specifically binds to the protein.
42 . A method for treating an infection in a subject, the method comprising: administering an effective amount of the composition of claim 28 to a subject.
43 . The method of claim 42 , wherein the subject has an infection caused by a P. multocida.
44 . The method of claim 42 , wherein the subject is a mammal.
45 . The method of claim 44 , wherein the mammal is a bovine.
46 . The method of claim 42 , wherein the subject is an avian.
47 . The method of claim 42 , wherein the avian is a chicken or a turkey.
48 . The method of claim 42 , wherein at least 0.01 micrograms and no greater than 3000 milligrams of protein is administered.
49 . A kit for detecting antibody that specifically binds a protein, comprising in separate containers:
the isolated protein of the composition of claim 28 ; and
a reagent that detects an antibody that specifically binds the protein.