Granted Patent B2

US 12,467,099 · App. 19/028,916 · Granted Nov 11, 2025

Inventors: Dietrich Stephan (San Francisco, CA); Vern Norviel (San Francisco, CA); Janet Warrington (Los Altos, CA); Doug Dolginow (Basalt, CO)

Assignee: Aqtual, Inc.

C12Q1/6886G01N33/57484C12Q2600/106C12Q2600/112C12Q2600/136C12Q2600/142C12Q2600/154C12Q2600/156C12Q2600/158C12Q2600/16

Abstract

Claims (29)

1 . A method comprising:

(a) obtaining a sample from a subject, wherein said sample comprises nucleic acid; and

(b) detecting methylation of a gene in said nucleic acid using massively parallel sequencing, wherein said massively parallel sequencing comprises use of reversibly terminating nucleotides.

2 . The method of claim 1 , further comprising treating said nucleic acid from said sample with bisulfite prior to (b).

3 . The method of claim 1 , wherein said method does not comprise treating said nucleic acid from said sample with bisulfite.

4 . The method of claim 1 , further comprising, prior to (b), preparing a whole genome library of nucleic acids from said nucleic acid of said sample.

5 . The method of claim 1 , wherein said sample is a tumor biopsy.

6 . The method of claim 1 , wherein said sample comprises cancer cells and wherein said method further comprises purifying said cancer cells from said sample.

7 . The method of claim 6 , wherein said purifying comprises laser capture microdissection.

8 . The method of claim 6 , wherein said purifying comprises flow sorting.

9 . The method of claim 1 , wherein said subject has a cancer.

10 . The method of claim 9 , wherein said cancer is a lung cancer, a non-small-cell lung cancer, a breast cancer, a prostate cancer, a gastric cancer, a head and neck cancer, an advanced gastric adenocarcinoma, a squamous cell carcinoma, a pancreatic adenocarcinoma, Hodgkin's Disease, non-Hodgkin's lymphoma, a testicular carcinoma, a colon cancer, a rectal cancer, an esophageal cancer, a hepatocellular carcinoma, a cholangiocarcinoma, a chronic myeloid leukemia, a chronic lymphocytic leukemia, a small lymphocytic lymphoma, or a follicular lymphoma.

11 . The method of claim 1 , wherein said methylation is associated with silencing of said gene.

12 . The method of claim 1 , further comprising generating a report comprising a treatment option, wherein said treatment option is based at least partially on said methylation of said gene.

13 . The method of claim 12 , wherein said treatment option comprises a drug treatment selected from the group consisting of imatinib, cetuximab, panitumumab, 5-FU, and temozolomide.

14 . The method of claim 1 , further comprising generating a report, wherein said report comprises a technique used for detecting said methylation.

15 . The method of claim 1 , further comprising:

obtaining an additional sample, wherein said additional sample comprises additional nucleic acid;

performing additional massively parallel sequencing of said additional sample; and

determining an additional methylation of an additional gene in said additional nucleic acid in said additional sample based on said additional massively parallel sequencing, wherein said additional massively parallel sequencing comprises use of reversibly terminating nucleotides.

16 . The method of claim 15 , further comprising:

comparing said methylation of said gene with said additional methylation of said additional gene;

determining a disease progression of said subject based on said comparing; and

generating a report comprising a treatment option, wherein said treatment option is based at least partially on said disease progression.

17 . The method of claim 1 , wherein said gene comprises a drug absorption gene, a drug distribution gene, a drug metabolism gene, or a drug excretion gene.

18 . The method of claim 1 , wherein said gene comprises a Cytochrome P450 (CYP450) gene.

19 . The method of claim 1 , further comprising detecting a presence or absence of a rearrangement, a nonsense mutation, a missense mutation, a silent mutation, a frameshift mutation, a point mutation, a single nucleotide polymorphism, an interstitial deletion, a chromosomal inversion, a loss of function mutation, a gain of function mutation, or a chromosomal translocation in said nucleic acid.

20 . The method of claim 1 , further comprising detecting a presence or absence of a substitution, an insertion, or a deletion in said nucleic acid.

21 . The method of claim 1 , wherein said massively parallel sequencing comprises bridge amplification.

Assignments (5)

Continuity (11)

References Cited (400)