SOLID SUPPORTS USEFUL FOR TISSUE ADHERENCE

1 . A flow cell assembly comprising:

a first solid support;

a resist attached to the first solid support, wherein the resist is a poly dimethylsiloxane (PDMS), poly(methyl methacrylate) (PMMA), cyclic olefin copolymer (COC), silsesquioxane resist, an epoxy-based polymer resist, poly(vinylpyrrolidone-vinyl acrylic acid) copolymer resist, an Off-stoichiometry thiol-enes (OSTE) resist, amorphous fluoropolymer resist, a crystalline fluoropolymer resist, polysiloxane resist, or an organically modified ceramic polymer resist;

a coupling agent attached to the resist;

a cell or tissue attached to the coupling agent;

a second solid support attached to the first solid support, wherein the second solid support is configured to define a reaction chamber when attached to the first solid support.

2 . The flow cell assembly of claim 1 , further comprising a frame configured to retain the flow cell assembly.

3 . The flow cell assembly of claim 2 , wherein the frame comprises a ferromagnetic pin.

4 . The flow cell assembly of claim 1 , further comprising a microchip.

5 . The flow cell assembly of claim 4 , wherein the microchip is an electronically erasable programmable read only memory (EEPROM) chip.

6 . The flow cell assembly of claim 1 , wherein the second solid support comprises a channel bored into the second solid support.

7 . The flow cell assembly of claim 1 , wherein the second solid support comprises a gasket, wherein the gasket defines the reaction chamber.

8 . The flow cell assembly of claim 7 , wherein the gasket comprises silicone, polyimide, fluorocarbon elastomer, ethylene propylene diene, polychloroprene, polytetrafluoroethylene, nitrile rubber, butyl rubber, natural rubber, thermoplastic elastomer, or a combination thereof.

9 . The flow cell assembly of claim 1 , further comprising 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 reaction chambers.

10 . The flow cell assembly of claim 1 , wherein the reaction chamber comprises a depth of about 50 μm to about 150 μm.

11 . The flow cell assembly of claim 1 , wherein the reaction chamber comprises a depth of about 80 μm to about 110 μm.

12 . The flow cell assembly of claim 1 , wherein the reaction chamber comprises a width of about 4 μm to about 15 μm.

13 . The flow cell assembly of claim 1 , wherein the second solid support is attached to the first solid support via a pressure sensitive adhesive.

14 . The flow cell assembly of claim 1 , wherein the tissue is embedded in an embedding material comprising paraffin wax, polyepoxide polymer, polyacrylic polymer, agar, gelatin, celloidin, cryogel, optimal cutting temperature (OCT) compositions, glycols, or a combination thereof.

15 . The flow cell assembly of claim 1 , wherein the first solid support or the second solid support comprises an inlet port and an outlet port.

16 . The flow cell assembly of claim 1 , wherein the first solid support or the second solid support further comprises an IR reflective coating.

17 . The flow cell assembly of claim 1 , wherein the first solid support further comprises a plurality of particles.

18 . The flow cell assembly of claim 1 , wherein the coupling agent comprises (3-aminopropyl)triethoxysilane (APTES), (3-Aminopropyl)trimethoxysilane (APTMS), γ-Aminopropylsilatrane (APS), N-(6-aminohexyl)aminomethyltriethoxysilane (AHAMTES), polyethylenimine (PEI), 5,6-epoxyhexyltriethoxysilane, or triethoxysilylbutyraldehyde, or a combination thereof.

19 . A method of making a flow cell assembly, the method comprising:

binding a resist to a first solid support;

binding a coupling agent to the resist;

attaching a cell or tissue to the coupling agent; and

affixing a second solid support to the first solid support, wherein the first solid support or the second solid support comprises an inlet port.

20 . A method of detecting a biomolecule in or on a cell or tissue, said method comprising:

immobilizing a cell or tissue comprising a biomolecule to a solid support, wherein said solid support comprises a resist attached to the solid support, and a coupling agent attached to the resist;

attaching a second solid support to the first solid support;

contacting the biomolecule in or on said cell or tissue with a detection agent comprising a label;

detecting the label, thereby detecting the biomolecule.