SYSTEMS, DEVICES, AND METHODS FOR AUTOMATIC CELL SORTING
The present disclosure relates to systems, devices, and methods for automated cell sorting within a cell processing system. In an embodiment, the present disclosure relates to an automated cell sorting system comprising a cartridge having a cell sorting module, where the cell sorting module comprises a flow cell, and an instrument within a bay of a cell processing workcell, where the instrument comprises a magnetic array couplable to the flow cell, each of the magnets within the magnetic array having a width of w and being spaced apart by between about w/3 to about ¾w, and wherein the flow cell has a height of between about 1/12w to about ⅛w.
1 .- 19 . (canceled)
20 . An automated cell sorting method comprising:
coupling a cell sorting module within a cartridge to a magnetic array of an instrument within a workcell; and
flowing a cell suspension through a flow cell of the cell sorting module in batches,
wherein the cell suspension comprises cells tagged by micrometer-sized or nanometer-sized magnetic particles and wherein each batch is maintained within the workcell for between about 3 and about 6 minutes.
21 . The cell sorting method of claim 20 , wherein a batch of the cell suspension has a volume of between about 8 mL to about 10 mL.
22 . The cell sorting method of claim 20 , further comprising decoupling the magnetic array by retracting a piston coupled to magnetic array.
23 . The cell sorting method of claim 22 , wherein decoupling the magnetic array is performed automatically.
24 . The cell sorting method of claim 20 , further comprising monitoring cell stiction within the flow cell using a prism and a sensor within the instrument.
25 . The cell sorting method of claim 24 , wherein monitoring cell stiction is performed automatically.
26 . The cell sorting method of claim 20 , further comprising purging the flow cell using a purge line.
27 . The cell sorting method of claim 26 , wherein purging the flow cell is performed automatically.
28 . The cell sorting method of claim 20 , wherein the nanometer-sized magnet particles have a diameter between about 50 nanometers to about 150 nanometers.
29 . The cell sorting method of claim 20 , wherein the micrometer-sized magnetic particles have a diameter between about 1 micron and about 6 microns.
30 . The cell sorting method of claim 20 , wherein between 1 to 20 batches are flowed through the flow cell.
31 . The cell sorting method of claim 20 , further comprising automatically transferring the magnetically tagged cells to a second module within the cartridge in accordance with a predefined workflow, wherein the second module is configured to perform a second cell processing step.
32 . An automated cell sorting method comprising:
moving a cartridge including a cell sorting module from a feedthrough to a bay of a workcell, wherein the workcell comprises an instrument including a magnetic array coupled to an actuator;
translating the magnetic array via the actuator to engage the cell sorting module;
flowing a cell suspension through a flow cell of the cell sorting module in batches;
wherein the cell suspension comprises cells that are tagged with micrometer-sized or nanometer-sized magnetic particles.
33 . The cell sorting method of claim 32 , wherein a batch of the cell suspension has a volume of between about 8 mL to about 10 mL.
34 . The cell sorting method of claim 32 , further comprising disengaging the cell sorting module by translating the magnetic array.
35 . The cell sorting method of claim 32 , further comprising monitoring cell stiction within the flow cell using a prism and a sensor within the instrument.
36 . The cell sorting method of claim 32 , further comprising purging the flow cell using a purge line.
37 . The cell sorting method of claim 32 , wherein the nanometer-sized magnet particles have a diameter between about 50 nanometers to about 150 nanometers.
38 . The cell sorting method of claim 32 , wherein the micrometer-sized magnetic particles have a diameter between about 1 micron and about 6 microns.
39 . The cell sorting method of claim 32 , wherein at least 1 to 20 batches are flowed through the flow cell.
40 . The cell sorting method of claim 32 , wherein the cell suspension comprises cells that are tagged with micrometer-sized and nanometer-sized magnetic particles.