ANTIBODY NEUTRALIZING HUMAN RESPIRATORY SYNCYTIAL VIRUS
The present invention relates to monoclonal antibodies which have high anti-RSV neutralizing titers. The invention further provides for isolated nucleic acids encoding the antibodies of the invention and host cells transformed therewith. The invention yet further provides for diagnostic, prophylactic and therapeutic methods employing the antibodies and nucleic acids of the invention, particularly as a passive immunotherapy agent in infants and the elderly.
1 - 25 . (canceled)
26 . A method of producing an antibody or antigen binding fragment comprising:
(a) culturing a host cell engineered to express one or more polypeptides of the antibody or antigen binding fragment thereof in a culture medium; and
(b) recovering the antibody or antigen binding fragment from the host cell and/or culture medium;
wherein the antibody or antigen binding fragment comprises a heavy chain variable region CDR1 comprising the amino acid sequence of SEQ ID NO:1, a heavy chain variable region CDR2 comprising the amino acid sequence of SEQ ID NO:2, a heavy chain variable region CDR3 comprising the amino acid sequence of SEQ ID NO:3, a light chain variable region CDR1 comprising the amino acid sequence of SEQ ID NO:4, a light chain variable region CDR2 comprising the amino acid sequence of SEQ ID NO: 5, and a light chain variable region CDR3 comprising the amino acid sequence of SEQ ID NO: 6.
27 . The method of claim 26 , wherein the antibody or antigen binding fragment thereof comprises a heavy chain variable region comprises the amino acid sequence of SEQ ID NO:7 and the antibody light chain variable region comprises the amino acid sequence of SEQ ID NO:8.
28 . The method of claim 26 , wherein the host cell comprises an expression vector having a polynucleotide encoding the heavy chain variable region of the antibody or antigen binding fragment thereof.
29 . The method of claim 26 , wherein the host cell comprises an expression vector having the polynucleotide encoding the light chain variable region of the antibody or antigen binding fragment thereof.
30 . The method of claim 26 , wherein the host cell comprises an expression vector having both a polynucleotide encoding the heavy chain variable region and the polynucleotide encoding the light chain variable region of the antibody or antigen binding fragment thereof.
31 . The method of claim 26 , wherein the host cell is a Pichia cell or a Chinese hamster ovary cell.
32 . The method of claim 26 , wherein the host cell is a Chinese hamster ovary cell.
33 . A method of preventing or treating an RSV disease, comprising administering to a subject an effective amount of an antibody or antigen binding fragment that binds to human RSV F protein, wherein the antibody or antigen binding fragment comprises a heavy chain variable region CDR1 comprising the amino acid sequence of SEQ ID NO:1, a heavy chain variable region CDR2 comprising the amino acid sequence of SEQ ID NO:2, a heavy chain variable region CDR3 comprising the amino acid sequence of SEQ ID NO:3, a light chain variable region CDR1 comprising the amino acid sequence of SEQ ID NO:4, a light chain variable region CDR2 comprising the amino acid sequence of SEQ ID NO:5, and a light chain variable region CDR3 comprising the amino acid sequence of SEQ ID NO:6.
34 . A method of detecting the presence of RSV in a sample, the method comprising,
a) contacting the sample to be tested for the presence of RSV with an anti-hRSV F protein antibody or antigen-binding fragment thereof,
wherein anti-hRSV F protein antibody or antigen-binding fragment thereof comprises a heavy chain variable region CDR1 comprising the amino acid sequence of SEQ ID NO:1, a heavy chain variable region CDR2 comprising the amino acid sequence of SEQ ID NO:2, a heavy chain variable region CDR3 comprising the amino acid sequence of SEQ ID NO:3, a light chain variable region CDR1 comprising the amino acid sequence of SEQ ID NO:4, a light chain variable region CDR2 comprising the amino acid sequence of SEQ ID NO:5, and a light chain variable region CDR3 comprising the amino acid sequence of SEQ ID NO:6; and
(2) detecting the antibody or fragment on or in the sample.