ANTIBODY COMPOSITIONS TARGETING NON-PHOSPHORYLATED ALPHA-SYNUCLEIN AGGREGATES
The present specification provides a monoclonal antibody that specifically binds aggregated, non-phosphorylated α-synuclein and a hybridoma producing it. Also disclosed are methods of generating antibodies that specifically binds aggregated, non-phosphorylated α-synuclein and uses thereof. Uses of anti-α-synuclein antibody in detection and diagnostic assays, and for prophylaxis or therapy of α-synuclein-associated neurodegenerative diseases, are also disclosed.
1 . A nucleic acid molecule comprising a sequence encoding a monoclonal antibody that specifically binds aggregated, non-phosphorylated α-synuclein comprising a heavy chain variable region (VH) and a light chain variable region (VL).
2 . A host cell comprising the nucleic acid molecule of claim 1 , wherein the host cell is capable of expressing the monoclonal antibody.
3 . The host cell of claim 2 , wherein the nucleic acid molecule encoding the monoclonal antibody is integrated into host cell genomic DNA.
4 . A vector comprising the nucleic acid molecule of claim 1 .
5 . A host cell comprising the nucleic acid molecule of claim 1 , wherein the host cell is capable of replicating the nucleic acid molecule.
6 . The nucleic acid molecule of claim 1 , wherein the sequence encoding the monoclonal antibody is cDNA.
7 . The nucleic acid molecule of claim 1 , wherein the sequence encoding the monoclonal antibody is mRNA.
8 . A nucleic acid molecule comprising a sequence encoding the monoclonal antibody of claim 7 .
9 . A host cell comprising the nucleic acid molecule of claim 8 , wherein the host cell is capable of expressing the monoclonal antibody.
10 . The host cell of claim 9 , wherein the nucleic acid molecule encoding the monoclonal antibody is integrated into host cell genomic DNA.
11 . A vector comprising the nucleic acid molecule of claim 8 .
12 . A host cell comprising the nucleic acid molecule of claim 8 , wherein the host cell is capable of replicating the nucleic acid molecule.
13 . The nucleic acid molecule of claim 8 , wherein the sequence encoding the monoclonal antibody is cDNA.
14 . The nucleic acid molecule of claim 8 , wherein the sequence encoding the monoclonal antibody is mRNA.
15 . An immunoassay for the detection or quantitation of aggregated, non-phosphorylated α-synuclein comprising a step for contacting a sample with the monoclonal antibody of claim 1 .
16 . A method of treating an α-synuclein-associated neurodegenerative disorder comprising administering the anti-α-synuclein antibody of claim 1 to a subject in need thereof.