POLYMER COATINGS FOR SOLID SUPPORTS
Disclosed herein, inter alia, are compositions and methods for making surface coatings for tissue adherence and/or retention.
1 . A solid support comprising a polymer and a cell or tissue section attached to the polymer, wherein the polymer comprises a subunit having the formula:
wherein
R 1 is hydrogen, halogen, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;
R 2 is hydrogen, halogen, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;
R 3 is hydrogen, halogen, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;
R 4 is —N 3 or —NR 5 R 6 ;
R 5 is hydrogen, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, or an amine protecting group;
R 6 is hydrogen, substituted or unsubstituted alkyl, or substituted or unsubstituted heteroalkyl, or an amine protecting group; and
L 1 is
wherein m is an integer from 0 to 24.
2 . The solid support of claim 1 , wherein R 4 is —NR 5 R 6 .
3 . The solid support of claim 1 , wherein R 4 is —NH 2 .
4 . The solid support of claim 1 , wherein L 1 is
wherein m is an integer from 6 to 24.
5 . The solid support of claim 1 , wherein L 1 is
wherein m is an integer from 2 to 12.
6 . The solid support of claim 1 , wherein L 1 is
7 . The solid support of claim 6 , wherein m is an integer from 4 to 12.
8 . The solid support of claim 6 , wherein m is an integer from 6 to 12.
9 . The solid support of claim 1 , wherein R 2 and R 3 are hydrogen.
10 . The solid support of claim 1 , wherein R 1 is hydrogen or substituted or unsubstituted alkyl.
11 . The solid support of claim 1 , wherein R 1 is unsubstituted alkyl.
12 . The solid support of claim 1 , wherein the polymer comprises a plurality of subunits having the formula:
wherein m is an integer from 2 to 24.
13 . The solid support of claim 1 , wherein the polymer comprises a plurality of subunits having the formula:
wherein m is an integer from 2 to 24.
14 . The solid support of claim 1 , further comprising a spacer subunit having the formula
wherein
R 7 is hydrogen, halogen, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;
R 8 is hydrogen, halogen, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;
R 9 is hydrogen, halogen, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;
R 10 is
hydrogen, halogen, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;
R 11 is hydrogen, substituted or unsubstituted alkyl, or substituted or unsubstituted heteroalkyl;
R 12 is hydrogen, substituted or unsubstituted alkyl, or substituted or unsubstituted heteroalkyl;
R 13 is hydrogen, substituted or unsubstituted alkyl, or substituted or unsubstituted heteroalkyl; and
L 2 is a covalent linker.
15 . The solid support of claim 14 , wherein the spacer subunit has the formula:
16 . The solid support of claim 1 , wherein the solid support comprises an IR reflective coating.
17 . The solid support of claim 1 , further comprising a resist, wherein the resist is between the polymer and the solid support.
18 . The solid support of claim 17 , wherein the resist is a polydimethylsiloxane (PDMS), poly(methyl methacrylate) (PMMA), cyclic olefin copolymer (COC), silsesquioxane resist, an epoxy-based polymer resist, poly(vinylpyrrolidone-vinyl acrylic acid) copolymer resist, an Off-stoichiometry thiol-enes (OSTE) resist, amorphous fluoropolymer resist, a crystalline fluoropolymer resist, polysiloxane resist, SU-8 resist, or an organically modified ceramic polymer resist.
19 . The solid support of claim 17 , wherein the resist is an organically modified ceramic polymer resist.
20 . A method of making a solid support, the method comprising: binding a first polymer to the solid support and contacting the first polymer with a reducing agent to generate a second polymer, wherein the first polymer comprises polymerized subunits having the formula:
and the second polymer comprises polymerized subunits having the formula:
wherein
R 1 is independently hydrogen, substituted or unsubstituted alkyl, or substituted or unsubstituted heteroalkyl;
R 2 is independently hydrogen or substituted or unsubstituted alkyl;
R 3 is independently hydrogen or substituted or unsubstituted alkyl;
L 1 is -L 1A -L 1B -L 1C -L 1E -; and
L 1A , L 1B , L 1C , L 1D , and L 1E are independently a bond, —NH—, —O—, —S—, substituted or unsubstituted alkylene, or substituted or unsubstituted heteroalkylene.