SYSTEMS, DEVICES, AND METHODS FOR COMBINED CELL PROCESSES
The present disclosure relates to systems, devices, and methods for spinoculation and counterflow centrifugal elutriation. In an embodiment, the present disclosure relates to a cartridge in an automated system, comprising a liquid transfer bus, a module fluidically coupled to the liquid transfer bus, the module comprising two sub-modules, each configured to perform separate cell processing steps, and at least one selector valve configured to direct fluid to at least one of the sub-modules.
1 .- 19 . (canceled)
20 . A method of processing cells in an automated system comprising:
coupling, via a robotic arm, a cartridge having cells therein to an instrument within a cell processing workcell;
transferring the cells from a liquid transfer bus of the cartridge via a first flow path to a module of the cartridge, the module comprising two sub-modules that share a rotor, each sub-module configured to perform separate cell processing steps;
performing at least one cell processing step within at least one of the sub-modules while the cartridge is coupled to the instrument; and
transferring the cells from the module to the liquid transfer bus via a second flow path.
21 . The method of claim 20 , wherein the two sub-modules comprise separation sub-module configured to perform cell separation and a transduction sub-module configured to perform cell transduction.
22 . The method of claim 21 , further comprising flowing a buffer through at least one sub-module while performing at least one cell processing step.
23 . The method of claim 22 , wherein the buffer maintains at least one seal in at least one of the first and second flow paths.
24 . The method of claim 21 , wherein the cell separation and cell transduction are performed series.
25 . The method of claim 23 , wherein the buffer is recirculated through at least one of the sub-modules.
26 . The method of claim 20 , further comprising measuring the rate of rotation of the rotor using at least one sensor.
27 . The method of claim 21 , further comprising generating image data of the separation sub-module using at least one sensor.
28 . The method of claim 27 , wherein the image data is generated via a viewing window of the module.
29 . The method of claim 20 , wherein the first and second fluid flow paths are at least partially integrated within the rotor.
30 . The method of claim 20 , wherein transferring the cells to the module comprises directing the cells to the at least one sub-module.
31 . The method of claim 30 , wherein the rotor comprises at least one integrated selector valve configured to direct the cells.
32 . The method of claim 31 , further comprising adjusting the at least one selector valve between different positions to define different fluid flow paths through the rotor.
33 . The method of claim 32 , wherein adjusting the at least one selector valve comprises rotating a gear coupled to the rotor using a controller of the cell processing workcell.
34 . The method of claim 32 , wherein the at least one selector valve comprises a first selector valve that is adjustable to define the first fluid flow path, and a second selector valve that is adjustable to define the second fluid flow path.
35 . The method of claim 21 , wherein the cell separation comprises magnetic separation.
36 . The method of claim 21 , wherein the cell transduction comprises spinoculation.