FAR-RED DYE PROBE FORMULATIONS
Disclosed are formulations, including both liquid and lyophilized formulations, comprising a far-red dye probe and a non-linear surfactant or foamban. Also disclosed are related methods for preparing a lyophilized far-red dye probe formulation as well as related kits and diagnostic products.
1 . A method of preparing a stabilized, aqueous far-red dye probe formulation, the method comprising:
dissolving a far-red dye probe comprising a far-red dye conjugated to a carrier molecule in a diluent to provide a stabilized, aqueous far-red dye probe formulation comprising:
the far-red dye probe;
a non-linear surfactant at a concentration from about 0.5% (v/v) to about 20% (v/v); and
at least one buffering agent;
wherein the far-red dye probe formulation is an aqueous solution.
2 . The method of claim 1 , wherein the diluent comprises the non-linear surfactant.
3 . The method of claim 1 , wherein the non-linear surfactant is selected from the group consisting of a polyoxyethylene sorbitan fatty acid ester and digitonin.
4 . The method of claim 1 , wherein the non-linear surfactant is a polyoxyethylene sorbitan fatty acid ester selected from the group consisting of polysorbate 20, polysorbate 40, and polysorbate 60.
5 . The method of claim 1 , wherein the non-linear surfactant concentration is from about 0.5% (v/v) to about 10% (v/v), from about 1% (v/v) to about 20% (v/v), or from about 1% (v/v) to about 10% (v/v).
6 . The method of claim 21 , wherein the far-red dye is a far-red cyanine dye.
7 . The method of claim 6 , wherein the far-red cyanine dye is selected from the group consisting of cyanine 5 and cyanine 5.5.
8 . The method of claim 1 , wherein the far-red dye probe in the stabilized, aqueous far-red dye probe formulation has a relative fluorescence, in relative fluorescence units (RFU), and the relative fluoresce of far-red dye probe is decreased by no more than 20% after 30 days of storage.
9 . The method of claim 1 , wherein the at least one buffering agent comprises Tris.
10 . The method of claim 9 , wherein the Tris buffering agent is present in the far-red dye probe formulation at a concentration of from about 5 mM to about 50 mM.
11 . The method of claim 1 , wherein the far-red dye probe is present in a lyophilized composition before being dissolved in the diluent.
12 . A diagnostic product comprising a sealed container containing a stabilized far-red dye probe formulation comprising:
a far-red dye probe comprising a far-red dye conjugated to a carrier molecule;
a non-linear surfactant at a concentration from about 0.5% (v/v) to about 20% (v/v); and
at least one buffering agent;
wherein the formulation is an aqueous solution, and further wherein:
(i) the non-linear surfactant is selected from the group consisting of a polyoxyethylene sorbitan fatty acid ester and digitonin; or
(ii) the far-red dye probe in the formulation has a relative fluorescence, in relative fluorescence units (RFU), and the relative fluoresce of far-red dye probe is decreased by no more than 20% after 30 days of storage.
13 . The diagnostic product of claim 12 , wherein the non-linear surfactant is selected from the group consisting of a polyoxyethylene sorbitan fatty acid ester and digitonin.
14 . The diagnostic product of claim 12 , wherein the non-linear surfactant is a polyoxyethylene sorbitan fatty acid ester selected from the group consisting of polysorbate 20, polysorbate 40, and polysorbate 60.
15 . The diagnostic product of claim 12 , wherein the non-linear surfactant concentration is from about 0.5% (v/v) to about 10% (v/v), from about 1% (v/v) to about 20% (v/v), or from about 1% (v/v) to about 10% (v/v).
16 . The diagnostic product of claim 12 , wherein the far-red dye is a far-red cyanine dye.
17 . The diagnostic product of claim 12 , wherein the far-red cyanine dye is selected from the group consisting of cyanine 5 and cyanine 5.5.
18 . The diagnostic product of claim 12 , wherein the far-red dye probe in the reconstituted stabilized, aqueous far-red dye probe formulation has a relative fluorescence, in relative fluorescence units (RFU), and the relative fluoresce of far-red dye probe is decreased by no more than 20% after 30 days of storage.
19 . The diagnostic product of claim 12 , wherein the stabilized far-red dye probe formulation is a stabilized, reconstituted far-red dye probe formulation.
20 . The diagnostic product of claim 12 , wherein the carrier molecule is a nucleic acid.