Method in Continuous Chromatography
The present invention relates to a method for purifying a target product in a flow-through chromatography system comprises at least a first column loaded with feed material from a feed source. The at least first column is purged after binding of impurities and wherein the outlet of purged material from the column is subsequently passed to the feed source.
1 . A flow-through chromatography system comprising at least a first column loaded or loadable with feed material from a feed source, wherein the flow-through chromatography system is configured:
to purge the at least first column after binding of impurities, and
to pass purged material from the outlet of the first column upstream of the first column to be re-purified.
2 . The flow-through chromatography system according to claim 1 , wherein the flow-through chromatography system is configured to pass purged material to the feed source.
3 . The flow-through chromatography system according to claim 1 , wherein the flow-through chromatography system is a continuous chromatography system comprising at least three chromatography columns, and wherein the flow-through chromatography system is further configured:
to flow at least partly purified feed material through the at least first column after binding of impurities and, and
to pass the partly purified feed material from the first column subsequently onto a second column for binding of impurities in the partly purified feed material.
4 . The flow-through chromatography system according to claim 1 , wherein the flow-through chromatography system is further configured to consecutively load each column.
5 . The flow-through chromatography system according to claim 1 , wherein the first column comprises a column inlet and a column outlet, wherein the column inlet and the column outlet are connected to a valve system.
6 . The flow-through chromatography system according to claim 5 , wherein the valve system is configured to achieve continuous purification of a target product.
7 . The flow-through chromatography system according to claim 5 , wherein the valve system comprises a purge outlet.
8 . The flow-through chromatography system according to claim 1 , further comprising one or more sensors, wherein the one or more sensors are configured to measure flow, VCD, weight, pressure, UV, volume, pH, conductivity, absorbance, or a combination thereof.
9 . The flow-through chromatography system according to claim 8 , wherein the one or more sensors comprises an in-line sensor.
10 . The flow-through chromatography system according to claim 1 , further comprising a buffer inlet.
11 . The flow-through chromatography system according to claim 1 , further comprising a waste outlet.