SCALABLE BIOREACTOR SYSTEMS AND METHODS FOR TISSUE ENGINEERING
Disclosed herein are scalable, modular bioreactor systems for efficient preparation of cell-based tissues. Also disclosed herein are methods, compositions, and apparatuses for preparing scaffolds.
1 . A tissue culturing device configured for production of a synthetic leather, the tissue culturing device comprising at least one cell culturing container, wherein the at least one cell culturing container comprises a scaffold, wherein the at least one cell culturing container comprises a fibroblast cell or a fibroblast expressing CD10, CD73, CD44, CD90, type 1 collagen, type III collagen, or any combination thereof, wherein the tissue culturing device is a fixed bed perfusion bioreactor.
2 . The tissue culturing device of claim 1 , wherein the tissue culturing device is temperature controlled.
3 . The tissue culturing device of claim 1 or 2 , wherein the tissue culturing device is jacketed.
4 . The tissue culturing device of anyone of claims 1 to 3 , wherein the tissue culturing device comprises a monitoring system.
5 . The tissue culturing device of claim 4 , wherein the monitoring system comprises a sensor, a camera, or a combination thereof.
6 . The tissue culturing device of claim 5 , wherein the monitoring system comprises the sensor, wherein the sensor comprises a thermistor, a thermometer, a pH sensor, a humidity sensor, a pressure sensor, a gas sensor, a CO 2 sensor, an O 2 sensor, a smoke detector, or any combination thereof.
7 . The tissue culturing device of any one of_ claims 1 to 6 , wherein the fibroblast cell or the fibroblast expressing CD10, CD73, CD44, CD90, type 1 collagen, type III collagen, or any combination thereof, comprises a plurality of fibroblast cells expressing CD10, CD73, CD44, CD90, type 1 collagen, type III collagen, or any combination thereof, in a cell layer.
8 . The tissue culturing device of claim 7 , wherein the cell layer is in contact with the scaffold.
9 . The tissue culturing device of any one of claims 1 to 8 , wherein the tissue culturing device comprises an agitator configured to mix growth medium within a container.
10 . The tissue culturing device of any one of claims 1 to 9 , wherein the tissue culturing device comprises at least one, two, three, four, five, six, seven, eight, ten, or fifteen openings or ports.
11 . A system comprising the tissue culturing device of any one of claims 1 to 10 and a cell culture medium reservoir.
12 . The system of claim 11 , wherein the cell culture medium reservoir is maintained at a lower temperature than the tissue culturing device.
13 . The system of claims 11 or 12 , wherein the cell culture medium is warmed before entering the
14 . The system of any one of claims 11 to 13 , wherein the tissue culturing device comprises an outlet for waste cell culture medium.