SYSTEMS AND DEVICES FOR WOUND THERAPY AND RELATED METHODS OF USE
Disclosed herein are systems and devices for wound therapy and related methods of use. The systems and devices for wound therapy and related methods of use disclosed herein may comprise one or more cartridges for preparation of a regenerative epidermal suspension. One or more cartridges for preparation of a regenerative epidermal suspension may comprise a cover with a raised processing opening, a cup that can be received within the raised processing opening and has a screen, and a well plate that is positioned under the cover, has a well, and rotates relative to the cover such that the well aligns with the raised processing opening. One or more cartridges for preparation of a regenerative epidermal suspension comprises a cover with an opening that can receive a tissue sample.
1 . A cartridge for preparation of a regenerative epidermal suspension, comprising:
a cover comprising a raised processing opening;
a cup configured to be received within the raised processing opening, wherein the cup comprises a screen; and
a well plate positioned under the cover and comprising a well, the well plate configured to rotate relative to the cover such that the well aligns with the raised processing opening.
2 . The cartridge of claim 1 , wherein the screen is positioned transversely within the cup.
3 . The cartridge of claim 1 , wherein the screen is configured to separate out particulates above 100 microns in size.
4 . The cartridge of claim 1 , wherein the screen is configured to separate out particulates above 50 microns in size.
5 . The cartridge of claim 1 , wherein the screen is configured to separate out particulates above 10 microns in size.
6 . The cartridge of claim 1 , further comprising a docking spindle configured to connect to a base unit.
7 . The cartridge of claim 6 , wherein the docking spindle is configured to rotate the well plate.
8 . The cartridge of claim 1 , wherein the well comprises raised processing elements configured to enhance disaggregation of a tissue sample.
9 . The cartridge of claim 8 , wherein the raised processing elements are configured to enhance disaggregation of a tissue sample subjected to vertical, rotational, or grinding forces.
10 . The cartridge of any one of claim 1 wherein the cup is configured to be raised or lowered within the raised processing opening to position the cup in the well.
11 . The cartridge of claim 1 , wherein the cover comprises a plurality of openings.
12 . The cartridge of claim 11 , wherein the cover comprises a buffer opening.
13 . The cartridge of claim 1 , wherein the well plate comprises a plurality of openings.
14 . The cartridge of claim 13 , wherein the well plate comprises a buffer well and an enzyme well.
15 . The cartridge of claim 14 , wherein the well plate is configured to rotate such that the buffer well is positioned beneath the buffer opening.
16 . The cartridge of claim 14 , wherein the well plate is configured to rotate such that the enzyme well is positioned beneath the raised processing opening.
17 . The cartridge of claim 1 , wherein the cup is configured to receive a tissue disaggregator.
18 . A cartridge for preparation of a regenerative epidermal suspension, comprising:
a cover comprising an opening configured to receive a tissue sample; and
a well plate positioned under the cover and comprising a well, the well plate configured to be rotated relative to the cover such that the well aligns with the opening.
19 . The cartridge of claim 18 , wherein the cartridge is configured to lower the tissue sample into the well aligned with the opening.
20 . The cartridge of claim 18 , wherein the well plate comprises a buffer well and an enzyme well, and the well plate is configured to rotate to align either the buffer well or the enzyme well with the opening.
21 . The cartridge of claim 18 , further comprising a cup receivable within the opening of the cover, the cup being moveable between a raised position above the well to a lowered position within the well.