Method of obtaining compositions comprising Y4 specific compounds
This invention provides an isolated nucleic acid molecule encoding a human Y4 receptor, an isolated protein which is a human Y4 receptor, vectors comprising an isolated nucleic acid molecule encoding a human Y4 receptor, mammalian cells comprising such vectors, antibodies directed to the human Y4 receptor, nucleic acid probes useful for detecting nucleic acid encoding human Y4 receptor, antisense oligonucleotides complementary to any sequences of a nucleic acid molecule which encodes a human Y4 receptor, pharmaceutical compounds related to human Y4 receptors, and nonhuman transgenic animals which express DNA encoding a normal or a mutant human Y4 receptor. This invention further provides methods for determining ligand binding, detecting expression, drug screening, and treatment involving the human Y4 receptor.
1. A method of obtaining a composition containing a compound that binds a human Y4 receptor wherein the method comprises
determining whether a chemical compound binds to a human Y4 receptor expressed on the surface of a mammalian cell transfected with a vector adapted for expressing the receptor in the cell, and if the compound binds to the receptor,
admixing the compound with a carrier to obtain the composition;
wherein the human Y4 receptor (1) has an amino acid sequence identical to the amino acid sequence shown in FIG. 1 (SEQ ID NO: 2), or (2) is encoded by a nucleic acid sequence identical to the receptor-encoding nucleic acid sequence contained in plasmid pcEXV-Y4 (ATTC Accession No. 75631).
2. A method of obtaining a composition containing a compound that binds a human Y4 receptor wherein the method comprises
screening compounds to identify compounds which interact with, and bind to a human Y4 receptor expressed on the surface of a mammalian cell transfected with a vector adapted for expressing the receptor in the cell, and if the compound interacts with and binds to the receptor,
admixing the compound with a carrier to obtain the composition;
wherein the human Y4 receptor (1) has an amino acid sequence identical to the amino acid sequence shown in FIG. 1 (SEQ ID NO: 2); or (2) is encoded by a nucleic acid sequence identical to the receptor-encoding nucleic acid sequence contained in plasmid pcEXV-Y4 (ATTC Accession No. 75631).
3. The method of any of claim 1 or 2 , wherein the composition is a pharmaceutical composition and the carrier is a pharmaceutically acceptable carrier.