Deuterated cyclosporine analogs and methods of making the same
View Patent ↗Cyclosporine derivatives are disclosed which possess enhanced efficacy and reduced toxicity over naturally occurring and other presently known cyclosporins and cyclosporine derivatives. The cyclosporine derivatives of the present invention are produced by chemical and isotopic substitution of the cyclosporine A (CsA) molecule by: (1) Chemical substitution and optionally deuterium substitution of amino acid 1; and (2) deuterium substitution at key sites of metabolism of the cyclosporine A molecule such as amino acids 1, 4, 9. Also disclosed are methods of producing the cyclosporine derivatives and method of producing immunosuppression with reduced toxicity with the disclosed cyclosporine derivatives.
1. A cyclosporin A derivative having the structure of Formula (II):
wherein the configuration of the MeBmt residue is as shown in FIG. 3 , compound 5 and R is an unsaturated straight or branched aliphatic carbon chain of from 2 to 3 carbons, prepared by a method comprising:
(a) protecting the β-alcohol of cyclosporin A, thereby forming an intermediate acetyl cyclosporin A;
(b) oxidizing the acetyl cyclosporin A to produce an intermediate acetyl cyclosporin A aldehyde;
(c) treating the intermediate acetyl cyclosporin A aldehyde in a Wittig reaction with a phosphorus ylide having the structural formula
RCH═PPh 3
wherein
R is as defined above to produce an acetyl cyclosporin A derivative having the structure of Formula (I):
wherein R is as defined above and the configuration of the MeBmt residue is as shown in FIG. 3 , compound 5; and
(d) hydrolyzing the acetyl cyclosporin A derivative having the structure of Formula (I) with a base.
2. The cyclosporin A derivative of claim 1 , wherein the oxidizing step is carried out with an oxidizing agent selected from the group consisting of ozone, potassium permanganate, and osmium tetroxide.
3. The cyclosporin A derivative of claim 2 , wherein the oxidizing agent is osmium tetroxide.
4. A cyclosporine A derivative having the structure of Formula (II):
wherein the configuration of the MeBmt residue is as shown in FIG. 3 , compound 5 and
R is a saturated or unsaturated, straight or branched, aliphatic carbon chain of from 2 to 3 carbons that is substituted with one or more deuterium atoms, prepared by the method comprising the steps of:
(a) protecting the β-alcohol of cyclosporin A , thereby forming an intermediate acetyl cyclosporin A;
(b) oxidizing the acetyl cyclosporin A to produce an intermediate acetyl cyclosporin A aldehyde;
(c) treating the intermediate acetyl cyclosporin A aldehyde in a Wittig reaction with a phosphorus ylide having the structural formula
RCH═PPh 3
to produce an acetyl cyclosporine A derivative having the structure of Formula (I):
wherein the configuration of the MeBmt residue is as shown in FIG. 3 , compound 5; and
(d) hydrolyzing the acetyl cyclosporin A derivative having the structure of Formula (I) with a base.
5. The cyclosporin A derivative of claim 4 , wherein the oxidizing step is carried out with an oxidizing agent selected from the group consisting of ozone, potassium permanganate, and osmium tetroxide.
6. The cyclosporin A derivative of claim 5 , wherein the oxidizing agent is osmium tetroxide.
7. A cyclosporin A derivative having the structure of Formula (II):
wherein the configuration of the MeBmt residue is as shown in FIG. 3 , compound 5 and R is a member selected from the group consisting of —D, —CH═CD-CD 3 , —CD═CD-CD 3 , —CH═CH—CH═CD-CD 3 , CD═CH—CD═CD-CD 3 , —CH═CHCH═CD 2 , —CD═CH—CD═CD 2 , —CH═CD 2 , —CD═CD 2 , —CH═CH 2 , —CH═CH—CD 3 , —CH═CH—CH 3 , —CH═CH—CH═CH—CH 3 , and —CH═CH—CH═CH 2 prepared by a method comprising:
(a) protecting the β-alcohol of cyclosporin A , thereby forming an intermediate acetyl cyclosporin A;
(b) oxidizing the acetyl cyclosporin A to produce an intermediate acetyl cyclosporin A aldehyde;
(c) treating the intermediate acetyl cyclosporin A aldehyde in a Wittig reaction with a phosphorus ylide having the structural formula
RCH═PPh 3
to produce an acetyl cyclosporin A derivative having the structure of Formula (I):
wherein the configuration of the MeBmt residue is as shown in FIG. 3 , compound 5; and
(d) hydrolyzing the acetyl cyclosporin A derivative having the structure of Formula (I) with a base.
8. The cyclosporin A derivative of claim 7 , wherein the oxidizing step is carried out with an oxidizing agent selected from the group consisting of ozone, potassium permanganate, and osmium tetroxide.
9. The cyclosporin A derivative of claim 8 , wherein the oxidizing agent is osmium tetroxide.