Method for preparing cell cultures from biological specimens for chemotherapeutic and other assays
View Patent ↗An improved method for preparing a cell culture is disclosed. The method includes culturing a multicellular tissue explant in the presence of growth medium that is substantially free of enzymes capable of digesting the explant and, subsequently, removing the explant at a predetermined time.
1. A method for assessing a response of tumor cells to an agent comprising:
(a) culturing a plurality of multicellular tumor tissue explants from a patient specimen in the presence of growth medium, so as to allow for growth of tumor cells into a monolayer;
(b) monitoring growth and morphology of the monolayer, and removing said explants from said growth medium when the monolayer is at about 10 to about 50 percent confluency and then growing the monolayer;
(c) suspending cells of said monolayer and inoculating the suspended cells in a plurality of segregated sites;
(d) treating each of the plurality of segregated sites with at least one active agent; and
(e) measuring the response of the cells in said plurality of sites to the at least one active agent, thereby assessing a response of the tumor cells.
2. The method of claim 1 , wherein the multicellular tumor tissue explants are prepared by mechanically fragmenting a tumor tissue sample.
3. The method of claim 1 , wherein the growth medium is substantially free of enzymes capable of digesting said explants.
4. The method of claim 1 , wherein the stromal cells are fibroblasts.
5. The method of claim 2 , wherein the tumor tissue sample is at least about 100 mg.
6. The method of claim 1 , wherein the multicellular tumor tissue explants measure about 1 mm 3 .
7. The method of claim 1 , wherein the percent confluency is determined by estimating an area of an observed field occupied by the monolayer.
8. The method of claim 1 , wherein the growth and/or cell morphology of the monolayer is monitored by phase contrast microscopy.
9. The method of claim 1 , wherein cell death in response to the at least one active agent is determined.
10. A method for predicting a response of a cancer patient to a plurality of candidate treatments, the method comprising:
preparing a plurality of multicellular tumor tissue explants from a cancer patient specimen;
culturing the multicellular tumor tissue explants in the presence of growth medium, so as to allow for growth of tumor cells into a monolayer;
monitoring growth and morphology of the monolayer, and removing the explants from the growth medium when the monolayer is at about 10 to about 50 percent confluency and then growing the monolayer;
suspending the cells of said monolayer and inoculating the suspended cells in a plurality of segregated sites;
treating each of the plurality of segregated sites with a candidate treatment; and
measuring the response of the cells in said plurality of sites to the candidate treatments, thereby predicting a response of the cancer patient to each of the plurality of candidate treatments.
11. The method of claim 10 , wherein the multicellular tumor tissue explants are prepared by mechanically fragmenting a tumor tissue sample.
12. The method of claim 10 , wherein the growth medium is substantially free of enzymes capable of digesting said explant.
13. The method of claim 10 , wherein the stromal cells are fibroblasts.
14. The method of claim 10 , wherein the tumor tissue sample is at least about 100 mg.
15. The method of claim 10 , wherein the multicellular tumor tissue explants measure about 1 mm 3 .
16. The method of claim 10 , wherein the percent confluency is determined by estimating an area of an observed field occupied by the monolayer.
17. The method of claim 10 , wherein the growth and/or cell morphology of the monolayer is monitored by phase contrast microscopy.
18. The method of claim 10 , wherein cell death in response to the plurality of active agents is determined.
19. The method of claim 10 , further comprising, comparing the response of the cells to each of the plurality of active agents.
20. The method of claim 1 , wherein the explants are removed from the growth medium when the monolayer is at about 15% to about 25% confluency.
21. The method of claim 10 , wherein the explants are removed from the growth medium when the monolayer is at about 15% to about 25% confluency.