Nogo-B receptor antagonists
View Patent ↗Nogo-B receptors bind to Nogo-B and mediate its biological function. We have discovered that Nogo-B receptor is a component of endothelial cells, and is highly expressed in intact blood vessels. The present invention provides compositions comprising the Nogo-B receptor and fragments and fusion proteins thereof. The present invention also relates to nucleic acids encoding the Nogo-B receptor and fragments and fusion proteins thereof, as well as vectors and cells comprising such nucleic acids. The present invention also relates to antibodies specific for the Nogo-B receptor and fragments and fusion proteins thereof. The present invention also provides methods for preventing, detecting and treating Nogo-B receptor-related diseases, disorders and conditions.
1. A composition comprising a fragment of a Nogo-B receptor that inhibits a biological activity of a Nogo-B receptor, wherein said fragment consists of residues 52-116 of SEQ ID NO: 1.
2. A fusion protein comprising a fragment of Nogo-B receptor and a heterologous protein component, wherein said fragment consists of residues 52-116 of SEQ ID NO: 1.
3. A nucleic acid molecule comprising a nucleotide sequence encoding the fusion protein of claim 2 .
4. A vector comprising the nucleic acid molecule of claim 3 .
5. A host cell comprising the nucleic acid molecule of claim 3 or the vector of claim 4 .
6. A method for producing a fusion protein, wherein said fusion protein comprises a fragment of Nogo-B receptor consisting of residues 52-116 of SEQ ID NO: 1, said method comprising culturing the host cell of claim 5 under suitable conditions in media so that the host cell produces the fusion protein and isolating the fusion protein.
7. A composition comprising a fragment of a Nogo-B receptor that inhibits a biological activity of a Nogo-B receptor, wherein said fragment consists of the amino acid sequence of SEQ ID NO: 14, SEQ ID NO: 16, or SEQ ID NO: 18.
8. A fusion protein comprising a fragment of Nogo-B receptor and a heterologous protein component, wherein said fragment consists of the amino acid sequence of SEQ ID NO: 14, SEQ ID NO: 16, or SEQ ID NO: 18.
9. A nucleic acid molecule comprising a nucleotide sequence encoding the fusion protein of claim 8 .
10. A vector comprising the nucleic acid molecule of claim 9 .
11. A host cell comprising the nucleic acid molecule of claim 9 or the vector of claim 10 .
12. A method for producing a fusion protein, wherein said fusion protein comprises a fragment of Nogo-B receptor consisting of the amino acid sequence of SEQ ID NO: 14, SEQ ID NO: 16, or SEQ ID NO: 18, said method comprising culturing the host cell of claim 11 under suitable conditions in media so that the host cell produces the fusion protein and isolating the fusion protein.