Human anti-amyloid beta peptide antibody and fragment of said antibody
View Patent ↗A human anti-amyloid β peptide (hereinafter referred to as “Aβ”) antibody that binds to Aβ to thereby inhibit aggregation of Aβ molecules, and a fragment of said antibody are provided. The antibody and a fragment thereof according to the present invention, comprising a variable region of a human-derived anti-Aβ antibody, strongly reacts with Aβ to thereby inhibit its aggregation and hence may be used as a medicament for the prophylaxis and treatment of Alzheimer dementia.
1. An isolated polynucleotide that encodes a human anti-amyloid β peptide 1-42 (Aβ42) antibody or antigen binding fragment thereof, wherein the antibody or antigen binding fragment comprises the heavy chain CDRs 1-3 of SEQ ID NOs: 72-74, respectively, and the light chain CDRs 1-3 of SEQ ID NOs: 77-79, respectively.
2. A recombinant expression vector comprising the isolated polynucleotide according to claim 1 .
3. An isolated host cell comprising the recombinant expression vector of claim 2 .
4. A method for producing a human anti-Aβ42 antibody or antigen binding fragment thereof comprising culturing the isolated host cell of claim 3 and recovering the human anti-Aβ42 antibody or antigen-binding fragment thereof produced by the host cell.
5. A composition comprising the isolated polynucleotide according to claim 1 .
6. The isolated polynucleotide according to claim 1 , wherein the polynucleotide encodes the H chain variable region amino acid sequence of SEQ ID NO: 71 and the L chain variable region amino acid sequence of SEQ ID NO: 76.
7. The isolated polynucleotide according to claim 1 , wherein said Aβ42 is fibrillar Aβ42.
8. The isolated polynucleotide according to claim 1 , wherein said antigen-binding fragment of the antibody is Fab, Fab', F(ab') 2 , scAb, or scFv-Fc.
9. An isolated polynucleotide coding for a human anti-amyloid β peptide 1-42 (Aβ42) antibody or an antigen binding fragment of the antibody, wherein the antibody or antigen binding fragment comprises:
a) a heavy chain variable region comprising CDRs 1-3 of SEQ ID NO: 72-74, and
b) a light chain variable region fragment of a human anti-Aβ42 antibody.
10. A recombinant expression vector comprising the isolated polynucleotide according to claim 9 .
11. An isolated host cell comprising the recombinant expression vector of claim 10 .
12. A method for producing a human anti-Aβ42 antibody or antigen binding fragment thereof comprising culturing the isolated host cell of claim 11 and recovering the human anti-Aβ42 antibody or antigen binding fragment thereof produced by the host cell.
13. A composition comprising the isolated polynucleotide according to claim 9 .
14. The isolated polynucleotide according to claim 9 , wherein said Aβ42 is fibrillar Aβ42.
15. The isolated polynucleotide according to claim 9 , wherein said antigen binding fragment of the antibody is Fab, Fab', F(ab') 2 , scAb, or scFv-Fc.