Stable and soluble antibodies
View Patent ↗The invention provides antibodies that are modified to reduce aggregration propensity, and methods of producing such antibodies. The present invention also provides particularly stable and soluble scFv antibodies and Fab fragments specific for TNF, which comprise specific light chain and heavy chain sequences that are optimized for stability, solubility, in vitro and in vivo binding of TNF, and low immunogenicity. The nucleic acids, vectors and host cells for expression of the recombinant antibodies of the invention, methods for isolating them and the use of said antibodies in medicine are also disclosed.
1. An antibody that binds specifically to human TNFα, comprising:
a. a variable light chain that has an Arginine at Aho position 50 and comprises the sequence of SEQ ID NO: 2; and
b. a variable heavy chain comprising the sequence of SEQ ID NO: 5.
2. The antibody of claim 1 , further having a linker comprising the sequence of SEQ ID NO: 7.
3. The antibody of claim 1 , wherein the antibody comprises the sequence of SEQ ID NO: 10.
4. A pharmaceutical composition comprising a therapeutically effective amount of the antibody of claim 1 and a pharmaceutically acceptable carrier.
5. An isolated nucleic acid molecule encoding the antibody of claim 1 .
6. A vector comprising the nucleic acid molecule of claim 5 .
7. A host cell comprising the vector of claim 6 .
8. The antibody of claim 1 , wherein the antibody is a Fab, Fab′, a F(ab) 2 , single-chain Fv (scFv), an Fv fragment, or a linear antibody.