Culture medium for cultivation of microorganisms
The present invention discloses culture medium unit doses for cultivating microorganisms comprising at least two compositions, each composition packaged in a composition unit dose of a predetermined amount, the composition unit doses being used for combining one of each composition unit dose forming the culture medium unit dose. The composition unit doses being packaged separately and individually until a time the culture medium unit dose is to be prepared for use for cultivation of microorganisms, wherein the time one of each composition unit dose are combined thereby forming the culture medium unit dose. The invention also discloses a method of manufacturing the composition unit doses, and a kit for cultivating microorganisms, the kit comprising a combination of the composition unit doses.
1. A kit for forming a single unit dose of a liquid culture medium for detection of Group B Streptococcus (GBS) by orange to red color development, said kit comprising:
a. A liquid culture medium unit dose comprising horse serum, in which all the ingredients for detection of GBS by said color development are mixed, except starch, and
b. Separately, a starch unit dose dried in a paper carrier;
Wherein one unit dose of each of (a) and (b) when combined forms said single unit dose of a culture medium for detection of GBS by orange to red color development.
2. The kit of claim 1 , wherein said unit dose of (a) further comprises peptone.
3. The kit of claim 1 , wherein said unit dose of (a) further comprises methotrexate.
4. The kit of claim 1 , wherein said unit dose of (a) further comprises magnesium sulfate.
5. The kit of claim 1 , wherein said unit dose of (a) further comprises glucose.
6. The kit of claim 1 , wherein said unit dose of (a) further comprises pyruvate.
7. The kit of claim 1 , wherein said unit dose of (a) further comprises phosphate and MOPS buffer, with said unit dose of (a) at pH 7.4.
8. The kit of claim 1 , wherein said unit dose of (a) further comprises crystal violet.
9. The kit of claim 1 , wherein said unit dose of (a) further comprises colistine sulfate.
10. The kit of claim 1 , wherein said unit dose of (a) further comprises metronidazole.
11. The kit of claim 1 , wherein said unit dose of (a) is contained in a tube.
12. The kit of claim 1 , wherein said unit dose of (a) is made by autoclaving a composition comprising peptone and phosphate and MOPS buffer and aseptically adding sterile horse serum to cooled-off said composition.
13. The kit of claim 1 , wherein said unit dose of (a) is made by adding a second composition comprising glucose and pyruvate to a first composition comprising peptone and phosphate and MOPS buffer, after autoclaving said first composition and aseptically adding sterile horse serum to cooled-off said composition.
14. The kit of claim 1 , wherein, at the time of GBS inoculation, said unit dose of (b) is added to said unit dose of (a).
15. The kit of claim 1 , wherein a plurality of said unit dose of (b) is packaged in a single container.
16. The kit of claim 1 , wherein said unit dose of (a) contains 0.5 g of agar per liter.
17. The kit of claim 1 , wherein said unit dose of (a) contains 25.0 g of peptone per liter.
18. The kit of claim 1 , wherein said unit dose of (a) contains 0.6 g of methotrexate sodium salt per liter.
19. The kit of claim 1 , wherein said unit dose of (a) contains 250 g of glucose and 100 g of sodium pyruvate per liter.
20. The kit of claim 1 , wherein said unit dose of (a) contains 20 g of anhydrous magnesium sulfate per liter.