Nucleic acid encoding DLL3 antibodies
View Patent ↗Novel modulators, including antibodies and derivatives thereof, and methods of using such modulators to treat proliferative disorders are provided.
1. A nucleic acid encoding a polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 212.
2. The nucleic acid of claim 1 comprising the nucleotide sequence of SEQ ID NO: 412.
3. A nucleic acid encoding a polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 213.
4. The nucleic acid of claim 3 comprising the nucleotide sequence of SEQ ID NO: 413.
5. A vector comprising at least one of (a) a nucleic acid encoding a polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 212, and (b) a nucleic acid encoding a polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 213.
6. The vector of claim 5 , further comprising a promoter that controls expression of the nucleic acid of (a) or the nucleic acid of (b).
7. The vector of claim 5 comprising the nucleic acid of (a) and the nucleic acid of (b).
8. The vector of claim 7 , further comprising a promoter that controls expression of the nucleic acid of (a) and the nucleic acid of (b).
9. The vector of claim 7 , further comprising a first promoter that controls expression of the nucleic acid of (a) and a second promoter that controls expression of the nucleic acid of (b).
10. The vector of claim 5 , wherein the at least one nucleic acid comprises a nucleotide sequence set forth as SEQ ID NO: 412.
11. The vector of claim 5 , wherein the at least one nucleic acid comprises a nucleotide sequence set forth as SEQ ID NO: 413.
12. A host cell comprising at least one of (a) a nucleic acid encoding a polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 212, and (b) a nucleic acid encoding a polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 213.
13. The host cell of claim 12 , which is a mammalian host cell selected from the group consisting of CHO cell, HEK 293 cell, BHK cell, NSO cell, SP2/0 cell, YO myeloma cell, P3X63 mouse myeloma cell, PER cell, and PER.C6 cell.
14. The host cell of claim 13 , which is a CHO cell.
15. The host cell of claim 12 comprising the nucleic acid of (a) and the nucleic acid of (b).
16. The host cell of claim 12 , further comprising a vector comprising the nucleic acid of (a) or the nucleic acid of (b).
17. The host cell of claim 16 , wherein the vector comprises a promoter that controls expression of the nucleic acid of (a) or the nucleic acid of (b).
18. The host cell of claim 12 , further comprising a vector comprising the nucleic acid of (a) and the nucleic acid of (b).
19. The host cell of claim 18 , wherein the vector comprises a promoter that controls expression of the nucleic acid of (a) and the nucleic acid of (b).
20. The host cell of claim 18 , wherein the vector comprises a first promoter that controls expression of the nucleic acid of (a) and a second promoter that controls expression of the nucleic acid of (b).
21. The host cell of claim 12 , wherein the host cell expresses the polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 212.
22. The host cell of claim 21 , which is a CHO cell.
23. The host cell of claim 12 , wherein the host cell expresses the polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 213.
24. The host cell of claim 23 , which is a CHO cell.
25. The host cell of claim 12 , wherein the host cell expresses the polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 212 and the polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 213.
26. The host cell of claim 25 , which is a CHO cell.
27. The host cell of claim 12 , wherein the host cell expresses an antibody comprising a light chain variable region comprising an amino acid sequence set forth as SEQ ID NO: 212.
28. The host cell of claim 12 , wherein the host cell expresses an antibody comprising a heavy chain variable region comprising an amino acid sequence set forth as SEQ ID NO: 213.
29. The host cell of claim 12 , wherein the host cell expresses an antibody comprising a light chain variable region comprising an amino acid sequence set forth as SEQ ID NO: 212 and a heavy chain variable region comprising an amino acid sequence set forth as SEQ ID NO: 213.
30. The host cell of claim 29 , which is a CHO cell.