Primer and probe for detecting chlamydia trachomatis, and method for detecting chlamydia trachomatis using same
View Patent ↗The present invention relates to an oligonucleotide which is designed on the basis of a nucleotide sequence shown in SEQ ID NO: 1 or SEQ ID NO: 2 and hybridizes with the endogenous plasmid gene of Chlamydia trachomatis , oligonucleotide primer and probe for detecting Chlamydia trachomatis , the detection method of Chlamydia trachomatis using said primer and probe, and a primer for detecting Chlamydia trachomatis of said oligonucleotide or the use to a probe design.
1. A method for detecting the presence or absence of Chlamydia trachomatis in a sample, the method comprising:
obtaining a sample from a human subject; mixing the sample with a first oligonucleotide primer consisting of the nucleotide sequence of SEQ ID NO: 3, and a second oligonucleotide primer consisting of the nucleotide sequence of SEQ ID NO: 4in a solution;
subjecting the solution to a nucleic acid amplification reaction; and
detecting the presence of Chlamydia trachomatis in the sample by detecting an amplification product or detecting the absence of Chlamydia trachomatis in the sample by detecting the absence of an amplification product.
2. The method according to claim 1 , wherein the nucleic acid amplification reaction is real-time PCR.
3. The method according to claim 1 , wherein the detecting comprises using an intercalator.
4. A method for detecting the presence or absence of Chlamydia trachomatis in a sample, the method comprising:
obtaining a sample from a human subject;
mixing the sample with a first oligonucleotide primer consisting of the nucleotide sequence of SEQ ID NO: 3, a second oligonucleotide primer consisting of the nucleotide sequence of SEQ ID NO: 4, and an oligonucleotide probe labeled with a reporter dye and a quencher fluorescent dye, in a solution;
subjecting the solution to a nucleic acid amplification reaction, wherein the oligonucleotide probe is capable of hybridizing to a region of an amplification product produced by the amplification reaction; and
detecting the presence of Chlamydia trachomatis in the sample by detecting an amplification product or detecting the absence of Chlamydia trachomatis in the sample by detecting the absence of an amplification product.
5. The method according to claim 4 , wherein the labeled oligonucleotide probe comprises the nucleotide sequence of SEQ ID NO: 8 or the complementary sequence thereof; and
wherein the detecting comprises detecting the reporter dye derived from the labeled oligonucleotide probe.
6. The method according to claim 1 , wherein the detecting comprises detecting the presence or absence of an amplification product by performing electrophoresis on the solution following the nucleic acid amplification reaction.
7. The method according to claim 4 , wherein the nucleotide sequence of the oligonucleotide probe consists of 10 to 50 contiguous nucleotides of the nucleotide sequence of SEQ ID NO: 1 or the complementary sequence thereof.