IP Library Granted Patent US 10,101,248
Granted Patent B1
US 10,101,248 · App. 15/787,185 · Granted Oct 16, 2018

Solution and method for adhering suspension components to a substrate

Inventors: Daniel Campton (Seattle, WA); Joshua Nordberg (Bainbridge Island, WA); Ronald Seubert (Sammamish, WA)
Assignee: RareCyte, Inc.
G01N1/30G02B21/34G01N2001/305
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Quick Facts
Patent No.
US 10,101,248
App. No.
15/787,185
Granted
Oct 16, 2018
Kind
B1
Abstract

This disclosure is directed to a method and an attachment solution for adhering a cytological or histological sample, such as buffy coat, to a substrate, such as a microscope slide. The attachment solution includes an attachment base, an anti-coagulant, and a nonsteroidal anti-inflammatory drug. The attachment base may be an alcohol, an acid, an oxidizer, an organohalogen, a ketone, or any combination thereof. Once a sample is obtained, the sample may be re-suspended in the attachment solution or the attachment solution may be added to the sample. The sample may then be dispensed onto an analysis platform as one or more droplets and cured.

Claims (8)

1. A solution consisting essentially of:

an alcohol having a final concentration of 60-70% by volume;

acetylsalicyclic acid having a final concentration of 100-1000 ug/mL; and

water,

wherein the final concentrations are the concentrations of the respective components when mixed with a sample.

2. The solution of claim 1 , wherein the alcohol is methanol.

3. The solution of claim 1 , further comprising a buffer.

4. The solution of claim 1 , further comprising a salt, wherein the solution is hypotonic relative to the sample.

Assignments (3)
RELEASE OF SECURITY INTEREST Recorded Dec 30, 2019
From: WESTERN ALLIANCE BANK, AN ARIZONA CORPORATION
To: RARECYTE, INC.
Reel/Frame 051445/0343 →
SECURITY INTEREST Recorded Mar 29, 2019
From: RARECYTE, INC.
To: WESTERN ALLIANCE BANK, AN ARIZONA CORPORATION
Reel/Frame 048746/0498 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 18, 2017
From: CAMPTON, DANIEL; NORDBERG, JOSHUA; SEUBERT, RONALD
To: RARECYTE, INC.
Reel/Frame 043896/0555 →
Continuity (1)
Continuation In Part 14956622 · Dec 2, 2015
Cited By (1)
US 12,657,722