IP Library › Granted Patent US 10,724,021
Granted Patent B1
US 10,724,021 · App. 16/868,472 · Granted Jul 28, 2020

Nucleic acid-guided nucleases

Inventors: Juhan Kim (Boulder, CO); Benjamin Mijts (Boulder, CO); Aamir Mir (Boulder, CO); Andrew Garst (Boulder, CO); Kyle Seamon (Boulder, CO)
Assignee: Inscripta, Inc.
C12N9/22C12N15/102C12N15/74C12N15/81C12N15/85C12N15/905C12N15/907C12N2310/531
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 10,724,021
App. No.
16/868,472
Granted
Jul 28, 2020
Kind
B1
Abstract

The present disclosure provides novel RNA-guided enzymes for making rational and direct edits to the genome of live cells.

Claims (10)

1. A coding sequence for a nucleic acid-guided nuclease comprising a nucleic acid sequence of any of SEQ ID Nos. 15, 16 or 17.

2. The coding sequence for the nucleic acid-guided nuclease of claim 1 comprising the nucleic acid sequence SEQ ID No. 15.

3. The coding sequence for the nucleic acid-guided nuclease of claim 2 , wherein a compatible guide RNA to the nucleic-acid-guided nuclease has an optimal crRNA variable loop comprising UAUU or UCUU.

4. The coding sequence for the nucleic acid-guided nuclease of claim 1 comprising the nucleic acid sequence SEQ ID No. 16.

5. The coding sequence for the nucleic acid-guided nuclease of claim 4 , wherein a compatible guide RNA to the nucleic-acid-guided nuclease has an optimal crRNA variable loop comprising UCUU or UAUU.

6. The coding sequence for the nucleic acid-guided nuclease of claim 1 comprising the nucleic acid sequence SEQ ID No. 17.

7. The coding sequence for the nucleic acid-guided nuclease of claim 6 , wherein a compatible guide RNA to the nucleic-acid-guided nuclease has an optimal crRNA variable loop comprising UCUU or UGUU.

8. The coding sequence for a nucleic acid-guided nuclease of claim 1 for editing in bacteria.

9. The coding sequence for a nucleic acid-guided nuclease of claim 1 for editing in yeast.

10. The coding sequence for a nucleic acid-guided nuclease of claim 1 for editing in isolated mammalian cells.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 6, 2020
From: KIM, JUHAN; MIJTS, BENJAMIN; MIR, AAMIR; GARST, ANDREW; SEAMON, KYLE
To: INSCRIPTA, INC.
Reel/Frame 052593/0596 →
Continuity (1)
Continuation 16714320 · Dec 13, 2019
Cited By (3)
US 12,195,749 US 12,201,699 US 12,338,436