Method and molecular diagnostic device for detection, analysis and identification of genomic DNA
At least one exemplary embodiment of the invention is directed to a molecular diagnostic device that comprises a cartridge configured to eject samples comprising genomic material into a microfluidic chip that comprises an amplification area, a detection area, and a matrix analysis area.
1. A cartridge, configured to deliver a sample comprising genomic material, comprising:
(i) a genomic separation and direction system, wherein the genomic separation and direction system comprises:
a reaction chamber; and
at least one binding and release substrate, wherein the at least one binding and release substrate lies within the reaction chamber and is configured to bind and release at least a portion of a sample comprising genomic material;
(ii) an ejector head, wherein a first channel connects the reaction chamber and a chamber in the ejector head, which ejector head chamber is configured to receive at least a portion of a sample comprising genomic material through the first channel from the genomic separation and direction system, and wherein the ejector head expels at least a portion of the received sample out of the cartridge as ejected sample droplets; and
(iii) two electrodes, wherein a first electrode is positioned in the reaction chamber and a second electrode is positioned in the channel connecting the reaction chamber and the ejector head.
2. The cartridge according to claim 1 , wherein the substrate comprises charge switch material.
3. The cartridge according to claim 1 , wherein the binding and releasing of at least a portion of the sample is in response to an electric voltage.
4. The cartridge according to claim 1 , wherein the binding and releasing of at least a portion of the sample is in response to a difference in ionic composition of a fluid in contact with the substrate, and wherein the sample is contained within the fluid.
5. The cartridge according to claim 1 , wherein the binding and releasing of at least a portion of the sample is in response to a difference in pH of a fluid in contact with the substrate, and wherein the sample is contained within the fluid.
6. The cartridge according to claim 1 , wherein the substrate is a particle or bead.
7. The cartridge according to claim 1 , wherein the substrate is magnetic or paramagnetic.
8. The cartridge according to claim 1 , wherein the substrate is bound to the inner surface of the reaction chamber.
9. The cartridge according to claim 1 , wherein the volume of at least a portion of the sample released from the substrate is reduced from the volume of the sample.