Methods for diagnosing systemic lupus erythematosus
The present invention provides methods of diagnosing and monitoring systemic lupus erythematosus.
1. A method of treating systemic lupus erythematosus in a human subject in need thereof, the method comprising:
(a) receiving a systemic lupus erythematosus risk score for the human subject, wherein the systemic lupus erythematosus risk score is produced by a method comprising the steps of:
(i) log normalizing a level of erythrocyte-bound C4d (EC4d) marker to produce a log normalized level of EC4d marker and multiplying the log normalized level of EC4d marker by a predetermined weighting coefficient to produce a weighted score for EC4d marker, wherein the level of EC4d marker is detected in a blood sample obtained from the human patient;
(ii) log normalizing a level of B-lymphocyte-bound C4d (BC4d) marker to produce a log normalized level of BC4d marker and multiplying the log normalized level of BC4d marker by a predetermined weighting coefficient to produce a weighted score for BC4d marker, wherein the level of BC4d marker is detected in a blood sample obtained from the human patient;
(iii) multiplying a level of anti-nuclear antibody (ANA) by a predetermined weighting coefficient to produce a weighted score for ANA, wherein the level of ANA is detected in a blood sample obtained from the human patient; and
(iv) combining the weighted score for EC4d marker, BC4d marker, and ANA to produce the systemic lupus erythematosus risk score;
wherein the systemic lupus erythematosus risk score does not include a score for a level of double-stranded DNA antibody;
(b) diagnosing the human subject with systemic lupus erythematosus based on the systemic lupus erythematosus risk score, predicting a likelihood the human subject has systemic lupus erythematosus based on the systemic lupus erythematosus risk score, monitoring systemic lupus erythematosus disease activity in the human subject based on the systemic lupus erythematosus risk score, or determining a level of systemic lupus erythematosus disease activity in the human subject based on the systemic lupus erythematosus risk score; and
(c) treating the human subject from step (b) with an effective amount of cyclophosphamide, a corticosteroid, mycophenolate, methotrexate, azathioprine, leflunomide, belimumab, or hydroxychloroquine.
2. The method of claim 1 , further comprising comparing the systemic lupus erythematosus risk score to a standard.
3. The method of claim 1 , wherein receiving the systemic lupus erythematosus risk score comprises receiving a comparison of the systemic lupus erythematosus risk score to a standard.
4. The method of claim 1 , wherein step (b) comprises diagnosing the human subject with systemic lupus erythematosus based on the systemic lupus erythematosus risk score.
5. The method of claim 1 , wherein step (b) comprises monitoring systemic lupus erythematosus disease activity in the human subject based on the systemic lupus erythematosus risk score.
6. A method of treating systemic lupus erythematosus in a human subject in need thereof, the method comprising:
(a) selecting a human subject having a diagnosis of systemic lupus erythematosus based on a systemic lupus erythematosus risk score, wherein the systemic lupus erythematosus risk score is produced by a method comprising the steps of:
(i) log normalizing a level of erythrocyte-bound C4d (EC4d) marker to produce a log normalized level of EC4d marker and multiplying the log normalized level of EC4d marker by a predetermined weighting coefficient to produce a weighted score for EC4d marker, wherein the level of EC4d marker is detected in a blood sample obtained from the human patient;
(ii) log normalizing a level of B-lymphocyte-bound C4d (BC4d) marker to produce a log normalized level of BC4d marker and multiplying the log normalized level of BC4d marker by a predetermined weighting coefficient to produce a weighted score for BC4d marker, wherein the level of BC4d marker is detected in a blood sample obtained from the human patient;
(iii) multiplying a level of anti-nuclear antibody (ANA) by a predetermined weighting coefficient to produce a weighted score for ANA, wherein the level of ANA is detected in a blood sample obtained from the human patient; and
(iv) combining the weighted score for EC4d marker, BC4d marker, and ANA to produce the systemic lupus erythematosus risk score;
wherein the systemic lupus erythematosus risk score does not include a score for a level of double-stranded DNA antibody;
(b) treating the human subject having the diagnosis of systemic lupus erythematosus based on the systemic lupus erythematosus risk score from (a) with an effective amount of cyclophosphamide, a corticosteroid, mycophenolate, methotrexate, azathioprine, leflunomide, belimumab, or hydroxychloroquine.
7. A method of treating systemic lupus erythematosus in a human subject in need thereof, the method comprising:
(a) receiving a systemic lupus erythematosus risk score for the human subject, wherein the systemic lupus erythematosus risk score is produced by a non-transitory computer-readable storage medium having instructions stored thereon which, when executed by the one or more processors, cause the one or more processors to perform operations comprising:
(i) receiving a level of double-stranded DNA antibody, a level of erythrocyte-bound C4d (EC4d) marker, a level of B-lymphocyte-bound C4d (BC4d) marker, and a level of anti-nuclear antibody (ANA), wherein the level of double-stranded DNA antibody, the level of EC4d marker, the level of BC4d marker, and the level of ANA is from a blood sample obtained from the human subject;
(ii) log normalizing the level of EC4d marker to produce a log normalized level of EC4d marker and multiplying the log normalized level of EC4d marker by a predetermined weighting coefficient to produce a weighted score for EC4d marker;
(iii) log normalizing the level of BC4d marker to produce a log normalized level of BC4d marker and multiplying the log normalized level of BC4d marker by a predetermined weighting coefficient to produce a weighted score for BC4d marker;
(iv) multiplying the level of ANA by a predetermined weighting coefficient to produce a weighted score for ANA; and
(v) combining the weighted score for EC4d marker, the weighted score for BC4d marker, and the weighted score for ANA to produce the systemic lupus erythematosus risk score;
wherein the systemic lupus erythematosus risk score does not include a score for a level of double-stranded DNA antibody;
(b) diagnosing the human subject with systemic lupus erythematosus based on the systemic lupus erythematosus risk score, predicting a likelihood the human subject has systemic lupus erythematosus based on the systemic lupus erythematosus risk score, monitoring systemic lupus erythematosus disease activity in the human subject based on the systemic lupus erythematosus risk score, or determining a level of systemic lupus erythematosus disease activity in the human subject based on the systemic lupus erythematosus risk score; and
(c) treating the human subject from step (b) with an effective amount of cyclophosphamide, a corticosteroid, mycophenolate, methotrexate, azathioprine, leflunomide, belimumab, or hydroxychloroquine.
8. The method of claim 7 , wherein the one or more processors perform operations comprising further comprising comparing the systemic lupus erythematosus risk score to a standard.
9. The method of claim 7 , wherein step (b) comprises diagnosing the human subject with systemic lupus erythematosus based on the systemic lupus erythematosus risk score.
10. The method of claim 7 , wherein step (b) comprises monitoring systemic lupus erythematosus disease activity in the human subject based on the systemic lupus erythematosus risk score.